A protein ‐centric view of in vitro biological model systems for Schizophrenia

Schematic diagram of stem-cell derived 2D SCZ culture models (left column) vs 3D SCZ brain organoid models (middle column) to engineer neural networks for translational medicine. SCZ patient cells are reprogrammed towards human induced pluripotent stem cells (hiPSC). Derived 3D brain organoids from hiPSCs serve as a model for further investigations. The application of proteomics and Post-translational Modification specific proteomics (PTMomics) on 3D organoids could contribute to a wealth of information regarding the underlying mechanisms of the diseases and cell responses toin vivo settings (right column). AbstractSchizophrenia (SCZ) is a severe brain disorder, characterized by psychotic, negative and cognitive symptoms, affecting 1% of the population worldwide. The precise aetiology of SCZ is still unknown; however, SCZ has a high heritability, and is associated with genetic, environmental and social risk factors. Even though the genetic contribution is indisputable, the discrepancies between transcriptomics and proteomics in brain tissues are consistently challenging the field to decipher the disease pathology. Here we provide an overview of the state of the art of neuronal 2D and 3D model systems that can be combined with proteomics analyses to decipher specific brain pathology and detection of alternative entry points for drug development.© AlphaMed Press 2021Significance StatementThis review article stresses the importance to implement complex 3D brain organoids (BOs) ...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research