O-102 Polymeric scaffold loaded with CD133+ BMDSCs for endometrial regeneration in Asherman ’s syndrome

AbstractStudy questionCan CD133+ bone marrow-derived stem cells (BMDSCs) loaded in polyethylene glycol diacrylate (PEGda) and gelatin divide and decidualize?Summary answerBiocompatible porous PEGda and gelatin scaffold provides a three-dimensional environment for CD133+ cells to attach, divide, and decidualizein vitro.What is known alreadyIntrauterine adhesions (IUA) develop due to acquired damages in the endometrium resulting in partial to complete endometrial dysfunction in the Asherman syndrome. Previous works from our group have demonstrated the engraftment of CD133+ BMDSCs and its paracrine effect on endometrial proliferation, improved endometrial thickness and clinical outcome in murine and human models of Asherman syndrome (AS).Study design, size, durationHuman CD133+ BMDSCs were obtained from refractory AS patients undergoing autologous cell therapy. Two different polymers PEGda and gelatin were analysed for their ability to form porous scaffold. CD133+ BMDSCs cell adhesion and division was analysed up to14 days, and its differentiation upon 8-Br-cAMP was evaluatedin vitro on day 5.In vivo biocompatibility was evaluated until week 5.Participants/materials, setting, methodsPorous PEGda and gelatin scaffolds were synthesized by cryogelation. Porosity, interconnectivity, and its distribution were characterized by scanning electron microscopy (SEM) and micro-computed tomography. Cell adhesion, growth, and morphology were analysed by SEM and fluorescence microscopy while d...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research