Study of blood glucose and insulin infusion rate in real-time in diabetic rats using an artificial pancreas system

by Omer Batuhan Kirilmaz, Akshay Radhakrishna Salegaonkar, Justin Shiau, Guney Uzun, Hoo Sang Ko, H. Felix Lee, Sarah Park, Guim Kwon Artificial pancreas system (APS) is an emerging new treatment for type 1 diabetes mellitus. The aim of this study was to develop a rat APS as a research tool and demonstrate its application. We established a rat APS using Medtronic Minimed Pump 722, Medtronic Enlite sensor, and the open artificial pancreas system as a controller. We tested different dilutions of Humalog (100 units/ml) in saline ranged from 1:3 to 1:20 and determined that 1:7 dilution works well for rats with ~500g bodyweight. Blood glucose levels (BGL) of diabetic rats fed with chow diet (58% carbohydrate) whose BGL was mana ged by the closed-loop APS for the total duration of 207h were in euglycemic range (70–180 mg/dl) for 94.5% of the time with 2.1% and 3.4% for hyperglycemia (>180mg/dl) and hypoglycemia (
Source: PLoS One - Category: Biomedical Science Authors: Source Type: research