Caffeine-induced bradycardia, death, and anxiety-like behavior in zebrafish larvae

ConclusionsGiven the anxiogenic effect of caffeine and light preference of zebrafish larvae, caffeine-induced changes in light –dark cycles could provide a new reliable marker for anxiety. Therefore, the “light–dark locomotion test” employed in this study is valuable for research on anxiogenic or anxiolytic substances. The test may allow simultaneous and automatic analysis of the locomotion of many fish under multip le conditions, thereby enabling evaluation of dose–response effects of caffeine.
Source: Forensic Toxicology - Category: Forensic Medicine Source Type: research