Viruses, Vol. 13, Pages 650: Broad-Spectrum Antiviral Activity of 3D8, A Nucleic Acid-Hydrolyzing Single-Chain Variable Fragment (scFv), Targeting SARS-CoV-2 and Multiple Coronaviruses In Vitro

Viruses, Vol. 13, Pages 650: Broad-Spectrum Antiviral Activity of 3D8, A Nucleic Acid-Hydrolyzing Single-Chain Variable Fragment (scFv), Targeting SARS-CoV-2 and Multiple Coronaviruses In Vitro Viruses doi: 10.3390/v13040650 Authors: Gunsup Lee Shailesh Budhathoki Geum-Young Lee Kwang-ji Oh Yeon Kyoung Ham Young-Jun Kim Ye Rin Lim Phuong Thi Hoang Yongjun Lee Seok-Won Lim Jun-Mo Kim Seungchan Cho Tai-Hyun Kim Jin-Won Song Sukchan Lee Won-Keun Kim The virus behind the current pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the etiology of novel coronavirus disease (COVID-19) and poses a critical public health threat worldwide. Effective therapeutics and vaccines against multiple coronaviruses remain unavailable. Single-chain variable fragment (scFv), a recombinant antibody, exhibits broad-spectrum antiviral activity against DNA and RNA viruses owing to its nucleic acid-hydrolyzing property. The antiviral activity of 3D8 scFv against SARS-CoV-2 and other coronaviruses was evaluated in Vero E6 cell cultures. Viral growth was quantified with quantitative RT-qPCR and plaque assay. The nucleic acid-hydrolyzing activity of 3D8 was assessed through abzyme assays of in vitro viral transcripts and cell viability was determined by MTT assay. We found that 3D8 inhibited the replication of SARS-CoV-2, human coronavirus OC43 (HCoV-OC43), and porcine epidemic diarrhea virus (PEDV). Our results revealed the proph...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research