Isolation and Differentiation of Amniotic Membrane Stem Cells Into Keratinocytes.

Isolation and Differentiation of Amniotic Membrane Stem Cells Into Keratinocytes. Cell Transplant. 2020 Jan-Dec;29:963689720964381 Authors: Lan DTP, Binh PT, Giang NTQ, Van Mao C, Chung DT, Van Diep N, Trung DM, Van Tran P Abstract The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy. The amniotic membrane stem cells can differentiate into multiple cell lineages; they have low immunogenicity and anti-inflammatory functions. This research aims to examine the protocols for the isolation of human amniotic membrane stem cells, including their phenotypic characterization and in vitro potential for differentiation toward keratinocytes. Human placentas were obtained from selected cesarean-sectioned births. We isolated amniotic stem cells by trypsin and collagenase B digestion and centrifuged with Percoll. After monolayer expansion of adherent cells, the cells were characterized by immunocytology with octamer-binding transcription factor 4 and differentiated into keratinocytes by treating the cells with insulin, hydrocortisone, BMP-4, and vitamin C. Protocol for isolation of stem cells from amniotic membrane has high efficiency. Differentiation markers of stem cells into keratinocytes, such as vimentin, cytokeratin (CK) 14, and CK19, were determined by reverse transcription-polymerase chain reaction increase over time in culture. Stem cells isolated from t...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research