Afferent Renal Innervation in Anti Thy1.1 Nephritis in Rats.

Afferent Renal Innervation in Anti Thy1.1 Nephritis in Rats. Am J Physiol Renal Physiol. 2020 Oct 05;: Authors: Rodionova K, Veelken R, Hilgers KF, Paulus EM, Linz P, Fischer MJM, Schenker M, Reeh PW, Tiegs G, Ott C, Schmieder RE, Schiffer M, Amann K, Ditting T Abstract Afferent renal nerves exhibit a dual function controlling central sympathetic outflow via afferent electrical activity and influencing intrarenal immunological processes by releasing peptides such as calcitonin-gene-related peptide (CGRP). We tested the hypothesis that increased afferent (ARNA) and efferent renal nerve activity (RSNA) occur with augmented release of CGRP in anti-Thy1.1 nephritis, in which enhanced CGRP release exacerbates inflammation. Nephritis was induced in Sprague-Dawley rats by intravenous injection of OX-7 antibody (1.75 mg/kg), animals were investigated neurophysiologically, electrophysiologically and pathomorphologically six days later. Nephritic rats exhibited proteinuria (169.3±10.2 mg/24 h), with increased RSNA (14.7±0.9 bursts/s vs. control 11.5±0.9 bursts/s; n = 11, P < 0.05). However, ARNA (spikes/s) decreased in nephritis (8.0±1.8 Hz vs. control 27.4±4.1 Hz, n = 11, P < 0.05). In patch-clamp recordings neurons with renal afferents from nephritic rats showed a lower frequency of high activity following electrical stimulation (43.4% vs. 66.4% in controls, P < 0.05). In vitro assays showed that renal tissue from nephritic rat...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research