Vitamin A supplementation boosts control of antibiotic-resistant Salmonella infection in malnourished mice

by Annica R. Stull-Lane, Kristen L. Lokken-Toyli, Vladimir E. Diaz-Ochoa, Gregory T. Walker, Stephanie A. Cevallos, Andromeda L. N. Winter, Ariel Del Hoyo Mu ñoz, Guiyan G. Yang, Eric M. Velazquez, Chun-Yi Wu, Renée M. Tsolis Disseminated disease from non-typhoidalSalmonella enterica strains results in>20% mortality globally. Barriers to effective treatment include emerging multidrug resistance, antibiotic treatment failure, and risk factors such as malnutrition and related micronutrient deficiencies. Individuals in sub-Saharan Africa are disproportionately affected by non-typhoidalS.enterica bloodstream infections. To inform a clinical trial in people, we investigated vitamin A as a treatment in the context of antibiotic treatment failure in a mouse model of vitamin A deficiency. Vitamin A-deficient (VAD) mice exhibited higher systemic bacterial levels with a multidrug-resistant clinical isolate in comparison to mice on a control diet. Sex-specific differences in vitamin A deficiency and disseminated infection withS.enterica serotype Typhimurium (S. Typhimurium) were observed. VAD male mice had decreased weight gain compared to control male mice. Further, infected VAD male mice had significant weight loss and decreased survival during the course of infection. These differences were not apparent in female mice. In a model of disseminatedS. Typhimurium infection and antibiotic treatment failure, we assessed the potential of two consecutive doses of vitamin A ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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Authors: Eve A, Aliero AA, Nalubiri D, Adeyemo RO, Akinola SA, Pius T, Nabaasa S, Nabukeera S, Alkali B, Ntulume I Abstract The current upsurge in resistance to conventional antibiotics, as well as high cost of orthodox medical treatment, called for the use of medicinal plants as an alternative therapy. This research was aimed at determining the antibacterial activity of Artocarpus heterophyllus seed extracts (Jackfruit as it is locally called) in the treatment of diarrhoea. Ethanolic and hexanolic seed crude extracts of the plant were screened for antidiarrhoeal activity against bacteria isolated from clinical sam...
Source: The Scientific World Journal - Category: Science Tags: ScientificWorldJournal Source Type: research
In this study, antibiotic-resistant strains from marine sediment (n = 50) and clams (n = 53) were analyzed (i) for their plasmid content using a PCR-based plasmid replicon typing (PBRT) kit and (ii) for the transferability of plasmid-associated antibiotic resistance (AR) traits by mating experiments. Fifteen of the thirty replicons targeted by the PBRT kit were detected in the isolates; 8/15 were identified in both sediment and clam isolates, although at different frequencies. The most frequent replicons in sediment (74%) and in clam strains (66%) alike, were FIA, FIB, or FII, which are associated with the IncF group, foll...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
The emergence of antibiotic-resistant Salmonella through mutations led to mismatch repair (MMR) deficiency that represents a potential hazard to public health. Here, four representative MMR-deficient Salmonella hypermutator strains and Salmonella Typhimurium LT2 were used to comprehensively reveal the influence of MMR deficiency on antibiotic resistance among Salmonella. Our results indicated that the mutation frequency ranged from 3.39 × 10–4 to 5.46 × 10–2 in the hypermutator. Mutation sites in MutS, MutL, MutT, and UvrD of the four hypermutators were all located in the essential and core function...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Infectious diarrheal diseases still account for high morbidity and mortality in the developing and underdeveloped countries. The present study was envisaged to document the etiology of bacterial enteropathogens in three tribal districts of Odisha from July, 2010 to September, 2013. A total of 1427 rectal swabs were collected and bacteriologically analyzed following standard procedure. Among 930 (65.2%) culture positive samples E.coli were 636 (44.6%), V.cholerae O1 were 146 (10.2%), Salmonella spp were 10 (0.7%), Shigella spp were 79 (5.5%) and Aeromonas spp were 59 (4.1%). From 729 Environmental Water sa...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion: New carbapenem/βLI combinations may be viable alternatives to antimicrobial combination therapy as they displayed high efficacy in vitro and in vivo. Meropenem/Avibactam and Meropenem/Relebactam should be tested on larger sample sizes with different carbapenemases before progressing further in its preclinical development. Introduction Carbapenem resistant Gram-negative bacteria have been gradually increasing in prevalence in recent years. In the United States, the latest CDC Antibiotic Resistance Threat Report indicates that Carbapenem-Resistant Enterobacteriaceae (CREs) are responsible for 9,000 a...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Cosmeri Rizzato1, Javier Torres2, Elena Kasamatsu3, Margarita Camorlinga-Ponce2, Maria Mercedes Bravo4, Federico Canzian5 and Ikuko Kato6* 1Department of Translation Research and of New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy 2Unidad de Investigación en Enfermedades Infecciosas, Unidades Médicas de Alta Especialidad Pediatría, Instituto Mexicano del Seguro Social, Mexico City, Mexico 3Instituto de Investigaciones en Ciencias de la Salud, National University of Asunción, Asunción, Paraguay 4Grupo de Investigación en Biología del C&aacut...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study we examined the influence of ultra-small gold and magnetite‑gold nanoparticles (NPs) stabilized with d,l-methionine, Fe3O4@Au@Met, on their antibacterial efficacy against three of twelve the worst bacterial family members included in the World Health Organization (WHO) list. In particular, gram-negative Acinetobacter baumannii, Salmonella enterica and gram-positive methicillin-resistant Staphylococcus aureus and Micrococcus luteus were tested. Apart from the synthesis, gold species reduction and NP stabilization, an excess of methionine has been used herein to detach ultra-small gold NPs from the Fe3O4@Au@M...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
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