Avian anti-NS1 IgY antibodies neutralize dengue virus infection and protect against lethal dengue virus challenge.
In this study, we used egg yolks from DENV-immunized geese to purify IgY antibodies specific to DENV NS1 epitopes. We determined that 2 anti-NS1 IgY antibodies, NS1-1 and NS1-8, were capable of neutralizing DENV infection in vitro. In addition, these antibodies did not cross-react with the DENV Envelope (E) protein nor enhance DENV or Zika virus (ZIKV) infection in vitro. Intriguingly, NS1-8, but not NS1-1, partially blocked NS1-induced endothelial dysfunction in vitro while neither antibody blocked binding of soluble NS1 to cells. Finally, prophylactic treatment of mice with NS1-8 conferred significant protection against lethal DENV challenge. Although further research is needed to define the mechanism of action of these antibodies, our findings highlight the potential of anti-NS1 IgY as a promising prophylactic approach against DENV infection.
PMID: 32979401 [PubMed - as supplied by publisher]
Source: Antiviral Research - Category: Virology Authors: O'Donnell KL, Espinosa DA, Puerta-Guardo H, Biering SB, Schiltz J, Nilles ML, Li J, Harris E, Bradley DS Tags: Antiviral Res Source Type: research
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