Unacylated Ghrelin Regulates Glucose-Sensitive Neurons Activity and Glycolipid Metabolism via Orexin-A Neurons in the Lateral Hypothalamic Area
The objective of the study was to investigate the regulatory actions of
unacylated ghrelin (UAG) on glucose-sensitive (GS) neurons and glycolipid
metabolism in the lateral hypothalamus area (LHA) and its involvement with
orexin-A-immunopositive neurons. The effects of UAG administered into the
LHA on GS neurons discharges and glycolipid metabolism were detected by
single neuron discharge recording, biochemical index analysis and
quantitative real-time PCR; the level of c-fos protein in
orexin-A-immunopositive neurons was observed using immunofluorescence
staining. UAG microinjected into the LHA activated glucose-inhibited
neurons, which were partially blocked by pre-administration of anti-orexin-A
antibody in the LHA. Furthermore, UAG microinjected into the LHA
significantly reduced serum triglycerides (TG), total cholesterol,
low-density lipoprotein cholesterol, blood glucose, insulin and hepatic TG
levels, while elevated serum high-density lipoprotein cholesterol levels.
UAG elevated the mRNA expression of carnitine palmitoyltransferase-1 and
reduced the mRNA expression of acetyl-CoA carboxylase-1 in the liver. The
above-mentioned effects of UAG were partially blocked by pre-administration
of anti-orexin-A antibody. The expressions of orexin-A and c-fos were
obs...
Source: Hormone and Metabolic Research - Category: Endocrinology Authors: Su, Manqing Yan, Meixing Yao, Jiatong Fang, Yanpeng Jin, Hong Gong, Yanling Tags: Endocrine Research Source Type: research
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