Viruses, Vol. 12, Pages 732: Phylogenetic and Timescale Analysis of Barmah Forest Virus as Inferred from Genome Sequence Analysis

Viruses, Vol. 12, Pages 732: Phylogenetic and Timescale Analysis of Barmah Forest Virus as Inferred from Genome Sequence Analysis Viruses doi: 10.3390/v12070732 Authors: Alice Michie Timo Ernst I-Ly Joanna Chua Michael D. A. Lindsay Peter J. Neville Jay Nicholson Andrew Jardine John S. Mackenzie David W. Smith Allison Imrie Barmah Forest virus (BFV) is a medically important mosquito-borne alphavirus endemic to Australia. Symptomatic disease can be a major cause of morbidity, associated with fever, rash, and debilitating arthralgia. BFV disease is similar to that caused by Ross River virus (RRV), the other major Australian alphavirus. Currently, just four BFV whole-genome sequences are available with no genome-scale phylogeny in existence to robustly characterise genetic diversity. Thirty novel genome sequences were derived for this study, for a final 34-taxon dataset sampled over a 44 year period. Three distinct BFV genotypes were characterised (G1–3) that have circulated in Australia and Papua New Guinea (PNG). Evidence of spatio-temporal co-circulation of G2 and G3 within regions of Australia was noted, including in the South West region of Western Australia (WA) during the first reported disease outbreaks in the state’s history. Compared with RRV, the BFV population appeared more stable with less frequent emergence of novel lineages. Preliminary in vitro assessment of RRV and BFV replication kinetics found that RRV repl...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research