Multifunctional Core-Shell Glyco-Nanoparticles for Galectin-3 Targeted Trigger responsive Combination Chemotherapy.

Multifunctional Core-Shell Glyco-Nanoparticles for Galectin-3 Targeted Trigger responsive Combination Chemotherapy. Biomacromolecules. 2020 Jun 02;: Authors: Balakrishnan B, Subramanian S, Mallia MB, Repaka K, Kaur S, Chandan R, Bhardwaj P, Dash A, Banerjee R Abstract Galectin-3 (gal-3) plays a crucial role in various cellular events associated to tumor metastasis and progression. In this direction, gal-3 binding core-shell glyco-nanoparticles based on citrus pectin (CP) have been designed for targeted trigger responsive combination drug delivery. Depolymerization via periodate oxidation in heterogeneous medium yielded low molecular weight dialdehyde oligomers (CPDA) of CP with gal-3 binding property (kd= 160.90 µM). CPDA based core-shell nanoparticles prepared to enhance the gal-3 binding specificity via multivalent ligand presentation have shown to reduce the homotypic cellular aggregation, tumor cell binding with endothelial cells and endothelial tube formation, the major steps involved in the progression of cancer. Immune-fluorescence and flow cytometric analysis confirmed significant reduction in gal-3 expression on MDA-MB 231 cancer cells upon incubation with nanoparticles. An on-demand tumor microenvironment responsive release of drugs at low pH and, high concentrations of glucose and glutathione prevailing in tumor milieu, was achieved by introducing cleavable Schiff's base, boronate ester and disulphide linkages within the ...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research