Experimental Analysis of T-Cell Epitopes for Designing Liver Cancer Vaccine Predicted by System Level Immunoinformatics Approach.

Experimental Analysis of T-Cell Epitopes for Designing Liver Cancer Vaccine Predicted by System Level Immunoinformatics Approach. Am J Physiol Gastrointest Liver Physiol. 2020 May 04;: Authors: Muhammad SA, Zafar S, Rizvi SZ, Imran I, Munir F, Jamshed MB, Ali A, Wu X, Shahid N, Zaeem M, Zhang Q Abstract Liver cancer (LC) is the worldwide disease and currently, due to the poor prognostic and therapeutic options of liver cancer, we investigated the T-cell epitopes as potential therapeutic vaccine candidates to get the benefit of experimental processes and utilize the complete ability of the immune system compared to other artificial ex vivo proliferation of T cells. Activation of T-cells targets and kills several tumors, developing a strong rationale for the improvement of immunotherapeutic strategies to cancer therapy. To predict T-cell epitopes for liver cancer, we designed a comprehensive immunoinformatics framework involving data mining, immunogenicity prediction, functional proteomic analysis, conservation studies, molecular modeling and in vivo validation analysis. We found the binding affinity of antigenic peptides with MHC-I molecules to control the cancerous activity. Five extracellular antigenic proteins including complement protein (C6), serotransferrin (TF), coagulation factor XIII B (F13B), serum albumin (ALB), and prothrombin (F2) were identified. We predicted and synthesized T-cell epitopes to HLA-A*01:01 allele of MHC C...
Source: Am J Physiol Gastroi... - Category: Gastroenterology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research