Surface modified electrospun poly(lactic acid) fibrous scaffold with cellulose nanofibrils and Ag nanoparticles for ocular cell proliferation and antimicrobial application
In this study, we developed surface modified poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) with silver nanoparticles (AgNPs) and cellulose nanofibrils (CNF) as scaffolds for cell proliferation and antimicrobial application. The AgNPs with a very low content (below 0.1%) were easily anchored on the surface of PLA EFMs by CNF, which endowed the scaffold with hydrophilicity and antibacterial ability. The in-vitro cell co-culture experiments showed that the scaffold had great biocompatibility to ocular epithelial cells, especially the scaffolds coated by CNF, which significantly proliferated cells. Furthermore, the antibacterial activity could reach>95% inhibiting Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) due to the implantation of AgNPs, and the antifungal activity was also outstanding with most of the Fusarium spp. inhibited. Hence, the developed PLA EFMs with CNF and AgNPs are promising ocular bandages to promote cell proliferation and kill infectious pathogens, exhibiting potential applications in ocular wound healing in the future.
Publication date: Available online 31 March 2020Source: Microbial PathogenesisAuthor(s): Shalee K. Carlson, David L. Erickson, Eric Wilson
Publication date: Available online 1 April 2020Source: Food ControlAuthor(s): Marie Frétin, Christophe Chassard, Céline Delbès, René Lavigne, Etienne Rifa, Sébastien Theil, Benoit Fernandez, Patrice Laforce, Cécile Callon
Publication date: Available online 31 March 2020Source: Small Ruminant ResearchAuthor(s): R. Venkatvasan, P.X. Antony, H.K. Mukhopadhyay, V. Jayalakshmi, V.M. Vivek Srinivas, J. Thanislass, Selvaraj Stephen
CONCLUSION: These results suggested that rHP-NAP possesses the potential for use as an adjuvant of dendritic cell-based vaccine in anti-melanoma treatment. PMID: 32224538 [PubMed - in process]
Conclusion: To facilitate transparent consent, single-centre rather than pooled outcome data should be utilized. PMID: 32223494 [PubMed - as supplied by publisher]
Authors: González-Martín M, Alberto Corbera J, Suárez-Bonnet A, Tejedor-Junco MT Abstract Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors - molecules that allow bacteri...
Contributors : Subhash C Verma ; Sankar Adhya ; Soumya G Remesh ; Michal HammelSeries Type : Expression profiling by high throughput sequencingOrganism : Escherichia coliNucleoid remodeling facilitated by DNA supercoiling results in changes in nucleoid configuration and involves nucleoid-associated proteins (NAPs) and structural maintenance of chromosomes (SMC) proteins among others. Changes in nucleoid configurations regulated by NAPs are synchronized with cellular adaptation and influence the simultaneous expression of several genes. HU, a ubiquitous bacterial histone-like protein, is among the most conserved and abunda...
We report the synthesis and expression measurement of ~9000 inducible promoter variants. By tagging individual variants with barcodes, we can measure the expression levels of all variants under both induced and uninduced conditions in a single pooled experiment. Sequencing data here is used for 1) paired-end sequencing to map barcodes to their promoters or 2) Quantifying the counts of barcodes at the DNA and RNA levels
Contributors : Pabitra Nandy ; Savita Chib ; Aswin S SeshasayeeSeries Type : Expression profiling by high throughput sequencingOrganism : Escherichia coliRNA Sequencing of wild type Eschrichia coli and a RNA polymerase mutant isolated from deep stationary phase
Contributors : Fernando H Martins ; Ashwani Kumar ; Chao Xing ; Vanessa Sperandio ; Waldir P EliasSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAtypical enteropathogenic Escherichia coli (aEPEC) is amongst the leading causes of diarrheal disease worldwide. The colonization of the gut mucosa by aEPEC results in actin pedestal formation at the site of bacterial attachment. This cytoskeletal rearrangement is triggered by the interaction between the bacterial adhesin intimin and its receptor Tir, which is translocated through the type three secretion system, to the host cell. While some...