Cultivable, Host-Specific Bacteroidetes Symbionts Exhibit Diverse Polysaccharolytic Strategies.

Cultivable, Host-Specific Bacteroidetes Symbionts Exhibit Diverse Polysaccharolytic Strategies. Appl Environ Microbiol. 2020 Feb 14;: Authors: Vera-Ponce de León A, Jahnes BC, Duan J, Camuy-Vélez LA, Sabree ZL Abstract Beneficial gut microbes can facilitate insect growth on diverse diets. The omnivorous American cockroach, Periplaneta americana (Insecta: Blattodea), thrives on a diet rich in plant polysaccharides and harbors a species-rich gut microbiota responsive to host diet. Bacteroidetes are among the most abundant taxa in P. americana and other cockroaches based on cultivation-independent gut community profiling and these potentially polysaccharolytic bacteria may contribute to host diet processing. Eleven Bacteroidetes isolates were cultivated from P. americana digestive tracts and phylogenomic analyses suggest that they were new Bacteroides, Dysgonomonas, Paludibacter and Parabacteroides species distinct from those previously isolated from other insects, humans and environmental sources. In addition, complete genomes were generated for each isolate and polysaccharide utilization loci (PUL) and several non-PUL associated CAZyme coding genes that putatively target starch, pectin and/or cellulose were annotated in each of the isolate genomes. Type IX secretion system (T9SS) and CAZyme coding genes tagged with the corresponding T9SS recognition and export C-terminal domain were observed in some isolates, suggesting that these C...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research