Roughened Graphite Biointerfaced with P450 Liver Microsomes: Surface and Electrochemical Characterizations

In this study, we have designed biointerfaces of human liver microsomes with various roughened, high-purity graphite disk electrodes to study electrochemical and electrocatalytic properties. Successful spectral and microscopic characterizations, direct bioelectronic communication, direct electron-transfer rates from the electrode to liver microsomal enzymes, microsomal heme-enzyme specific oxygen reduction currents, and voltage-driven diclofenac hydroxylation (chosen as the probe reaction) are presented.Graphical Abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research