MicroRNA profiles in celiac patients distinguish different clinical phenotypes and are modulated by gliadin peptides in primary duodenal fibroblasts

In this study the duodenal mucosa microRNA expression was profiled in adult untreated CD presenting with a classic phenotype or iron deficiency anemia, treated patients with or without duodenal normalization, and non-CD subjects as controls. Deregulation of seven miRNAs (miR-31-5p, miR-192-3p, miR-194-5p, miR-551a, miR-551b-5p, miR-638, and miR-1290) could be determined in a larger series of CD patients with different clinical phenotypes compared to non-CD subjects. These 7 microRNAs were then analyzed in duodenal fibroblasts obtained from CD patients and incubated with gliadin peptides (13 and 33mer). The microRNA cluster miR-192/194, involved in matrix remodeling, was deregulated in CD according to the different clinical presentations and miR-192-3p levels were modulated by gliadin peptides in vitro. The analysis of microRNAs deserves further consideration for its potential use in CD treatment and management.

Source: Clinical Science - September 24, 2013 Category: Biomedical Science Authors: V Vaira, L Roncoroni, D Barisani, G Gaudioso, S Bosari, G Bulfamante, L Doneda, D Conte, C Tomba, M Bardella, S Ferrero, M Locatelli, L Elli Source Type: research