Pantoea Natural Product 3 is encoded by an eight-gene biosynthetic gene cluster and exhibits antimicrobial activity against multi-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa

Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Ashley N. Williams, John StavrinidesAbstractMulti-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa continue to pose a serious health threat worldwide. Two Pantoea agglomerans strains, 3581 and SN01080, produce an antibiotic effective against these pathogens. To identify the antibiotic biosynthetic gene clusters, independent genetic screens were conducted for each strain using a mini-Tn5 transposon, which resulted in the identification of the same conserved eight-gene cluster. We have named this antibiotic Pantoea Natural Product 3 (PNP-3). The PNP-3 biosynthetic cluster is composed of genes encoding two Major Facilitator Superfamily (MFS) transporters, an ArsR family regulator, and five predicted enzymes. The biosynthetic gene cluster is found in only a few Pantoea strains and is not present within the antiSMASH and BAGEL4 databases, suggesting it may be novel. In strain 3581, PNP-3 production is linked to pantocin A production, where loss of pantocin A production results in a larger PNP-3 zone of inhibition. To evaluate the spectrum of activity, PNP-3 producers, including several PNP-3 mutants and pantocin A site-directed mutants, were tested against a collection of clinical, drug-resistant strains of A. baumannii and P. aeruginosa, as well as, Klebsiella, Escherichia coli, Enterobacter, Staphylococcus aureus, and Streptococcus mutans. PNP-3 was found to be effective...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research

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In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevert...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, wastewater effluents from the investigated poultry slaughterhouses exhibited clinically relevant bacteria (E. coli, MRSA, K. pneumoniae, species of the ACB-and E. cloacae-complexes) that contribute to the dissemination of clinically relevant resistances (i.e. bla CTX-M/SHV, mcr-1) in the environment.Importance Bacteria from livestock may be opportunistic pathogens and carriers of clinically relevant resistance genes, as many antimicrobials are used both in veterinary and human medicine. They may be released into the environment from wastewater treatment plants (WWTPs) that are influenced by wastewater from s...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
CONCLUSIONS: According to our results, when antimicrobial susceptibility tests are performed using Vitek 2 cards, the most common pathogens are correctly identified for the most frequent clinical isolates. PMID: 32161020 [PubMed - in process]
Source: Annals of Clinical and Laboratory Science - Category: Laboratory Medicine Authors: Tags: Ann Clin Lab Sci Source Type: research
Conclusion: Sufentanil Citrate, midazolam, and pethidine exerted efficient inhibitory effect on Staph. aureus, Strep. pneumoniae, P. aeruginosa and E. faecalis. Lidocaine and bupivacaine had a strong inhibitory effect on growth of nine standard bacterial species in our survey.
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: BACTERIOLOGY Source Type: research
CONCLUSIONS: The key clinically important finding is the rapidly decreasing carbapenem susceptibility among Enterobacteriaceae reported in SA, irrespective of sector. In addition, the analysis provides information that could be used to monitor the effectiveness of interventions implemented at a national level under the guidance and direction of the national AMR framework. PMID: 31865955 [PubMed - in process]
Source: South African Medical Journal - Category: African Health Tags: S Afr Med J Source Type: research
Abstract The threat of diminished antibiotic discovery has global healthcare in crisis. In the United States, it is estimated each year that over 2 million bacterial infections are resistant to first-line antibiotic treatments and cost in excess of 20 billion dollars. Many of these cases result from infection with the ESKAPE pathogens ( Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.), which are multidrug-resistant bacteria that often cause community- and hospital-acquired infections in both healthy and immunocompromised pati...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Abstract Antibiotics, considered as a backbone of modern clinical-medicines, are facing serious threats from emerging antimicrobial-resistance (AMR) in several bacteria from nosocomial and community origins and is posing a serious human-health concern. Recent commitment by the Heads of States at the United Nations General Assembly (UNGA, 2016) for coordinated efforts to curb such infections illustrates the scale of this problem. Amongst the drug-resistant microbes, major threat is posed by the group named as ESKAPEE, an acronym for Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter b...
Source: Fitoterapia - Category: Biochemistry Authors: Tags: Fitoterapia Source Type: research
Conclusion. The hexane extract of L. apiculata leaves showed the lower MIC against S. aureus when compared with extracts obtained from other parts of the plant. The growth curve and the colonies count suggest a bacteriostatic activity of the L. apiculata leaves extract against Staphylococcus aureus. PMID: 31737073 [PubMed]
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Publication date: Available online 31 October 2019Source: Food BioscienceAuthor(s): Esmeray Kuley, Mebrure Nüket Yavuzer, Emre Yavuzer, Mustafa Durmuş, Hatice Yazgan, Yekta Gezginç, Fatih ÖzogulAbstractThe impact of safflower (Carthamus tinctorius) and bitter melon (Momordica charantia L.) extracts on growth of fish spoilage bacteria (Acinetobacter lwoffii, Pseudomonas oryzihabitans, Enterobacter cloacae, Shigella spp., Morganella psychrotolerans, Photobacterium phosphoreum) and food-borne pathogens (Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis, Salmonella Paratyphi A) and their bio...
Source: Food Bioscience - Category: Food Science Source Type: research
Conclusion. In hospital-acquired bacterial meningitis, multidrug resistance Gram negative bacteria are a huge challenge for the treatment of patients. Hence, antimicrobial stewardship should be followed to counteract with the emerging multidrug resistance isolates. PMID: 31647400 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
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