GSE138114 Glioma-derived miRNA-containing extracellular vesicles induce angiogenesis by reprogramming brain endothelial cells (miRNA-seq)

Contributors : Rocco D Lucero ; Valentina Zappulli ; Alessandro Sammarco ; Oscar D Murillo ; Pike S Cheah ; Srimeenakshi Srinivasan ; Eric Tai ; David T Ting ; Zhiyun Wei ; Matthew E Roth ; Louise C Laurent ; Anna M Krichevsky ; Xandra O Breakefield ; Aleksandar MilosavljevicSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensGlioblastoma multiforme (GBM) is characterized by the close relationship of glioma stem cells (GSC) with aberrant vascularization. It has been established that GSC-derived extracellular vesicles (GSC-EVs) and their cargoes are proangiogenic in vitro. To elucidate gene regulatory mechanisms of neovascularization both in vitro and in vivo, we performed RNA-seq and DNA methylation profiling of the response of human brain endothelial cells to GSC-EVs as well as histoepigenetic analysis of GBM molecular profiles in the TCGA collection. The gene regulatory responses showed a footprint of post-transcriptional gene silencing by EV-derived miRNAs. Remarkably, EVs and normal vascular growth factors stimulated highly distinct gene regulatory responses, both converging on angiogenesis, providing exciting new insight into targetable angiogenic signaling in GBM.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research