Molecular characterization and expression of CD48 in Nile tilapia (Oreochromis niloticus) in response to different stimulus

In this study, the CD48 gene of tilapia (Oreochromis niloticus, named On-CD48), was cloned from the head kidney of tilapia. The coding sequences is 654 bp and encoding 217 amino acids. The deduced amino acid sequence of On-CD48 with an estimated molecular weight of 24.4 kDa and a theoretical pI of 5.03. Amino acid alignment indicated that it had two immunoglobulin-like domain conserved region. In healthy tilapia, the On-CD48 could be detected in all the examined tissues and the highest expression level in the spleen. The expression of On-CD48 in the spleen and head kidney was decreased after immunized by formalin-inactivated Streptococcus agalactiae, and the peak was observed in the spleen at 24 h and appeared again at 96 h, and in the head kidney gradual decline before 48 h then gradually increased to the original level. qPCR analysis of inactivated S. agalactiae, LPS and Poly I:C stimulated at the whole lymphocyte level showed that the stimulation of the Poly I:C was more sensitive. Prokaryotic expression results showed that efficient expression of On-CD48 protein could be realized after induced with 0.5 mmol L−1 IPTG in E. coli BL21 (DE3) for 10 h at 18 °C. The result of subcellular localization showed that On-CD48 were evenly distributed in the whole cell of HEK-293T. Western Blot confirmed that the molecular weight of the recombinant On-CD48 was about 21 kDa, consistent with the predicted result. The results of this study will lay a strong f...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research

Related Links:

Publication date: Available online 21 January 2020Source: Inorganica Chimica ActaAuthor(s): Mohammed Ismael, Abdel-Mawgoud M. Abdel-Mawgoud, Mostafa K. Rabia, Aly AbdouAbstractNew Fe(III) complexes with mixed ligands based on 1-{(E)-[(4-methylphenyl)imino]methyl}-2-naphthol (HN) as primary ligand and secondary co-ligand of O-hydroxy quinolone (HQ), 2-(1H-benzimidazol-2-yl)phenol (HB) and 2-(4,5-diphenyl-1H-imidazol-2-yl)phenol (HI) had been isolated and characterized. The isolated complexes had 1:1:1 ratio for Fe(III):ligand:co-ligand with one chloride and one water molecule coordinated to the Fe-centre, suggesting octahed...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
In this study, the removal of Eth-Br from aqueous solutions by gamma irradiation has been fully investigated. Gamma irradiation was capable of achieving a near complete removal of Eth-Br in neutral and non-buffered aqueous solutions at an absorbed dose of 15 kGy. Various experimental conditions were studied and showed that the removal efficiency is not diminished. The addition of hydrogen peroxide (2%) to the irradiated solutions reduced the D50 and D90 by 50%. Modeling Eth-Br decomposition showed that the reaction followed pseudo first-order kinetics and reaches at least 90% removal under all experimental conditions. TOC ...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
In this study, three tribenzoporphyrazines with dendrimeric peripheral substituents were subjected to in vitro antimicrobial photocytotoxicity study. One new magnesium(II) tribenzoporphyrazine with peripheral 3,5-bis(3,5-dimethoxybenzyloxy)benzylsulfanyl substituents was synthesized and subjected to physicochemical characterization using NMR, UV–Vis, and mass spectrometry techniques. In photochemical studies this molecule revealed moderate singlet oxygen generation ability (ΦΔDMF = 0.12, ΦΔDMSO = 0.13). The other two magnesium(II) tribenzoporphyrazines applied in the biological study were ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: Available online 20 January 2020Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Chandan Hunsur Ravikumar, R. Shwetharani, R. Geetha BalakrishnaAbstractCdSe/CdS core shelled quantum dots (QDs) were prepared by colloidal synthesis using a binary ligand system and a non-coordinating, reusable solvent n-octadecane (nOD). Both the synthesis of CdSe and CdSe/CdS core shelled quantum dots were achieved by hot injection technique at much lower temperatures than reported earlier. The use of binary ligand facilitated enough nucleation and growth. Red shift in absorption spectra, an enhanced ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: Available online 21 January 2020Source: Microbial PathogenesisAuthor(s): Xiaohui Mu, Ruxia Gao, Wenhua Xiao, Qingqing Gao, Chunguang Cao, Huiqinq Xu, Song Gao, Xiufan LiuAbstractExtraintestinal pathogenic Escherichia coli (ExPEC) shows an enhanced ability to cause infection outside the intestinal tract. Avian pathogenic E. coli (APEC), one type of ExPEC, causes avian colibacillosis, a disease of significant economic importance to poultry producers worldwide that is characterized by systemic infection. Some ExPEC strains as well as other pathogenic enterobacteria produce enterobactin, a catecholate siderop...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ou Boué Each year in Europe, meat is associated with 2.3 million foodborne illnesses, with a high contribution from beef meat. Many of these illnesses are attributed to pathogenic bacterial contamination and inadequate operations leading to growth and/or insufficient inactivation occurring along the whole farm-to-fork chain. To ensure consumer health, decision-making processes in food safety rely on Quantitative Microbiological Risk Assessment (QMRA) with many applications in recent decades. The present study aims to conduct a critical analysis of beef QMRAs and to identify future challenges. A systematic app...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
ConclusionOur findings argue against a co-selection mechanism of antimicrobial drug-resistance and zinc tolerance after dietary zinc supplementation in weaning piglets. An explanation for an increase in multi-drug resistant isolates from piglets with high zinc dietary feeding could be that resistant bacteria to antimicrobial agents are more persistent to stresses such as zinc or copper exposure.
Source: Gut Pathogens - Category: Microbiology Source Type: research
AbstractGiardia muris has been reported from both laboratory and wild rodents in worldwide. During routine care, soft consistency was observed in feces of Swiss albino mice. MotileGiardia spp. trophozoites were observed microscopically on all fecal preparations after stained with Lugol ’s iodine solution. The trophozoites were broadly ovoid in a form with a pointed end. They possess two large nuclei and an adhesive disk whose length overlapped one-half of the trophozoite body length. The caudal flagella are unequal. β giardin (bg) gene region of isolates was successfully amplified and sequenced. According to seq...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Mycobacteria are unique in many aspects of their biology. The development of genetic tools to identify genes critical for their growth by forward genetic analysis holds great promises to advance our understanding of their cellular, physiological and biochemical processes. Here we report the development of a novel transposon, MycoTetOP2, to aid the identification of such genes by direct transposon mutagenesis. This mariner-based transposon contains nested anhydrotetracycline (ATc)-inducible promoters to drive transcription outward from both of its ends. In addition, it includes the Escherichia coli R6Kγ origin to faci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, the detection of two different NDM-5 E. coli strains from a pediatric patient with a history of travel to the Far East countries strongly highlight an increasing trend and risk of importation from such areas.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Allergy & Immunology | Biology | Fish | Gastroenteritis | Genetics | Study | Urology & Nephrology