Riboflavin and pyrroloquinoline quinone generate carbon monoxide in the presence of tissue microsomes or recombinant human cytochrome P-450 oxidoreductase (CPR); implications for possible roles in gasotransmission.

Riboflavin and pyrroloquinoline quinone generate carbon monoxide in the presence of tissue microsomes or recombinant human cytochrome P-450 oxidoreductase (CPR); implications for possible roles in gasotransmission. Can J Physiol Pharmacol. 2019 Dec 11;: Authors: Vukomanovic D, Jia Z, Nakatsu K, Smith GN, Ozolinš TRS Abstract Carbon monoxide (CO), an endogenously produced gasotransmitter, regulates inflammation and vascular tone, suggesting delivery of CO may be therapeutically useful for pathologies like preeclampsia where CO insufficiency are implicated. Our strategy is to identify chemicals that increase the activity of endogenous CO producing enzymes, including cytochrome P-450 oxidoreductase (CPR). Realizing both riboflavin and pyrroloquinoline quinone (PQQ) are relatively non-toxic, even at high doses, and they share chemical properties with toxic CO activators we previously identified, our goal was to determine whether riboflavin or PQQ could stimulate CO production. Riboflavin and PQQ were incubated in sealed vessels with rat and human tissue extracts and CO generation was measured with headspace-gas chromatography. Riboflavin and PQQ increased CO production ~ 60% in rat spleen microsomes. In rat brain microsomes, riboflavin and PQQ increased respective CO production ~four-fold and two-fold, compared to baseline. CO production by human placenta microsomes increased four-fold with riboflavin and five-fold with PQQ. In the presence of recombi...
Source: Canadian Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Can J Physiol Pharmacol Source Type: research

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Conclusion: This analysis provided new information that was previously unavailable in this country. Furthermore, this tool proved to be a valid base for future registry aiming to consolidate the body of knowledge about CO intoxications in Italy.
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
The objective of the analysis was to clarify whether the time- and effect-adjusted nonlethal threshold concentration LCt01 × 1/3 from acute rat inhalation studies is suited to model thresholds characterizing any ‘impairment of escape’ in humans. Modeled outcomes are compared with published reference data from human volunteers exposed at the similar C × t's of CO at 800 ppm × 1-h and 100 ppm × 8-h. These exposure durations match the maximum escape duration of 1-h considered in the ISO standard 13571 and standards enforcing occupational exposure limits of 8-h duration. ...
Source: Regulatory Toxicology and Pharmacology - Category: Toxicology Source Type: research
Conclusions: Inflammatory pathways associated with pulmonary dysfunction in PLWH suggest multifaceted immune dysregulation involved in different phenotypes of pulmonary dysfunction with a potential specific contribution of the Th17 pathway to airway obstruction in PLWH. Identification of these associations may help in development of treatments that could alter the course of the disease.
Source: JAIDS Journal of Acquired Immune Deficiency Syndromes - Category: Infectious Diseases Tags: Translational Research Source Type: research
Heme oxygenase (HO)-1 plays an important role during hibernation by catalyzing the degradation of heme to biliverdin/bilirubin, ferrous iron, and carbon monoxide, which activates the protective mechanisms against stress. In this context, it was important to analyze the metabolic processes of heme. Nevertheless, to date, no study has approached on biosynthesis of heme. Therefore, our study aims to understand the process of heme biosynthesis, which regulates cell survival in conditions of hypothermia and calorie restriction (CR). During hibernation, the mRNA levels of enzymes responsible for de novo heme biosynthesis were in...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
oxford Background: Carbon monoxide (CO) poisoning is an important public health issue around the world. Research indicates that many factors may be related to the rate of CO uptake and elimination in the human body. However, some factors related to CO uptake and elimination are considered controversial. Relatively little attention has been devoted to review and synthesis of factors affecting CO uptake and elimination. Purpose: This paper provides a critical scoping review of the factors and divides them into four aspects, including environmental, demographic, physiological and treatment factors. Methods: We searched th...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
Publication date: Available online 13 January 2020Source: Materials Today: ProceedingsAuthor(s): Ashish Dewangan, Ashis Mallick, Ashok Kumar Yadav, Rajeev KumarAbstractIn transportation sector the most consumption fuel is Diesel. Combustion of diesel fuel in engine causes serious air pollution which includes carbon dioxide (CO2), unburned hydrocarbon (HC), carbon monoxide (CO), nitrogen oxide (NOx) and some particulate matters discharged from the combustion chamber. This emission causes health issues to the human and adversely affects the ecosystem. Several alternative fuels such as dimethyl ether, alcohol, hydrogen and bi...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
Carbon monoxide (CO) poisoning occurs due to CO gas which is produced by the incomplete combustion of hydrocarbons. Several causes of CO poisoning have been defined in the literature. The most frequent causes are defective heaters, fires and exposure to ex...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Occupational Issues Source Type: news
Publication date: Available online 9 January 2020Source: Sensors and Actuators B: ChemicalAuthor(s): Claudia gonzalez Viejo, Sigfredo Fuentes, Amruta Godbole, Bryce Widdicombe, Ranjith R UnnithanAbstractThe assessment of aromas in beer is critical to assess its quality since it could be used as an indicator of contamination or faults, which will directly influence consumers’ acceptability. Traditional techniques to evaluate aromas are time-consuming, require special training, costly equipment, and trained personnel. Therefore, this study aimed to develop a portable, low-cost electronic nose (e-nose) coupled with mach...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Publication date: Available online 10 January 2020Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Debabrata Nandi, Jyotishkumar Parameswaranpillai, Suchart SiengchinAbstractNickel manganate nanorod (NNM) decorated reduced graphene oxide (NNMG) was developed and its structure-property correlations in light of X-ray diffraction (XRD), Raman, field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) were evaluated. The starring role of NNM and reduced graphene oxide (RGO) in NNMG for CO ...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
Publication date: Available online 9 January 2020Source: Catalysis TodayAuthor(s): Fernando Fresno, Sandra Galdón, Mariam Barawi, Elena Alfonso-González, Carlos Escudero, Virginia Pérez-Dieste, Cristián Huck-Iriart, Víctor A. de la Peña O’SheaAbstractThe hydrothermal synthesis of the perovskites NaNbO3, NaTaO3 and the intermediate composition NaNb0.5Ta0.5O3, as CO2 conversion photocatalysts is reported. Among them, the niobate shows the most promising performance under UV irradiation not only in terms of conversion and light utilization ability, but also regarding the selecti...
Source: Catalysis Today - Category: Chemistry Source Type: research
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