Sodium nitroprusside attenuates hyperproliferation of vascular smooth muscle cells from spontaneously hypertensive rats through the inhibition of overexpression of AT1 receptor, cell cycle proteins and c-Src/growth factor receptor signaling pathways.
Abstract We recently showed that sodium nitroprusside (SNP), an NO donor, attenuated hypertension in spontaneously hypertensive rats (SHR). Since hypertension is associated with enhanced prolifera-tion and hypertrophy of vascular smooth muscle cells (VSMC), the present study examines whether in vivo treatment of SHR with SNP could also inhibit the augmented proliferation of VSMC and explore the signaling mechanisms. Treatment of 8-week-old SHR and WKY rats with SNP twice a week for two weeks inhibited the enhanced proliferation of VSMC from SHR, the enhanced expression of AT1 receptor, enhanced activation of c-Src...
Source: Canadian Journal of Physiology and Pharmacology - October 2, 2019 Category: Drugs & Pharmacology Authors: Hossain E, Sarkar O, Li Y, Anand-Srivastava MB Tags: Can J Physiol Pharmacol Source Type: research

Chronic metformin intake improves anaerobic but not aerobic capacity in healthy rats.
Abstract The effect of chronic metformin intake on aerobic and anaerobic capacity was examined in healthy rats. Twenty rats completed 10 days of metformin ingestion (MET, 250 mg). After this period, the animals performed 4 high-intensity bouts until exhaustion at 9, 11, 13 and 15% of body weight (BW) in swimming, separated by 24h hours, with prior metformin (250 mg) or placebo (PL). The Critical Load (CL) and Anaerobic Work Capacity (AWC) were calculated and considered aerobic and anaerobic capacity, respectively. There was no difference in CL between MET and PL group (p>0.05). The AWC was higher in MET than PL...
Source: Canadian Journal of Physiology and Pharmacology - October 2, 2019 Category: Drugs & Pharmacology Authors: de Araujo G, Learsi S, Bastos-Silva VJ, Ataide T, Lima-Silva AE Tags: Can J Physiol Pharmacol Source Type: research

Association of ABCB1 polymorphisms with lipid homeostasis and liver injury response to atorvastatin in Chinese population.
Abstract The present research was to assess the relationship between ABCB1 (G2677T/A, C3435T) polymorphisms and lipid homeostasis, as well as risk of liver injury induced by atorvastatin in inpatients from China. The lipids levels (TC, HDL, TG) as well as metabolic enzyme of hepar (ALT, AST, ALP, GGT) in plasma for 162 patients were measured at baseline and after approximately six months of atorvastatin treatment. Polymorphisms of the ABCB1 gene were determined using the Snapshot technique. The associations between genetic polymorphisms and lipids levels, as well as hepar indexes were evaluated at the end of medic...
Source: Canadian Journal of Physiology and Pharmacology - October 1, 2019 Category: Drugs & Pharmacology Authors: Qu KK, Zhang C, Dong LX, Wang SS, Zhang L, Zhang ZD Tags: Can J Physiol Pharmacol Source Type: research

Taurine and cardiac disease: state of the art and perspectives.
Abstract Taurine is a non-essential amino acid that has received much attention. Two organs, the heart, and the brain, are known to produce their own taurine, but in very limited quantities. It is for this reason that supplementation with this amino acid is necessary. Today, taurine is present in almost all energy drinks. A very vast literature reported beneficial effects of taurine in hepatic dysfunction, gastrointestinal injury, kidney diseases, diabetes, and cardiovascular diseases. Most of its effects were attributed to its modulation of Ca2+ homeostasis as well as to its antioxidant properties. In this review...
Source: Canadian Journal of Physiology and Pharmacology - September 27, 2019 Category: Drugs & Pharmacology Authors: Bkaily G, Jazzar A, Normand A, Simon Y, Al-Khoury J, Jacques D Tags: Can J Physiol Pharmacol Source Type: research

β-sitosterol mitigates the progression of high-fructose diet-induced non-alcoholic fatty liver disease in growing male Sprague-Dawley rats.
This study interrogated the potential protective effect of β-sitosterol against NAFLD in growing rats fed a high-fructose diet, modelling children fed obesogenic diets. Forty-four 21-day old male rat pups were randomly allocated to and administered the following treatments for 12 weeks: group I- standard rat chow (SRC) + plain drinking water (PW) + plain gelatine cube (PC); group II- SRC+ 20% w/v fructose solution (FS) as drinking fluid + PC; group III- SRC + FS + 100 mg/kg fenofibrate in gelatine cube; group IV- SRC + FS + 20 mg/kg β-sitosterol gelatine cube (Bst) and group V- SRC + PW + Bst. Terminally, the liv...
Source: Canadian Journal of Physiology and Pharmacology - September 27, 2019 Category: Drugs & Pharmacology Authors: Gumede N, Lembede B, Nkomozepi P, Brooksbank R, Erlwanger KH, Chivandi E Tags: Can J Physiol Pharmacol Source Type: research

Effects of Somatostatin, Curcumin and Quercetin on the fatty acid profile of breast cancer cell membranes.
Abstract We used MCF-7 and MDA-MB231 breast cancer cells incubated with Curcumin and Quercetin for 24h, in the absence and presence of Somatostatin, at their EC50 concentrations, to evaluate membrane fatty acid-based functional lipidomics together with the follow-up of EGFR and MAPK signaling pathways. The two cell lines gave different membrane free fatty acid reorganization: in MCF-7 cells, the following changes observed: increase of omega-6 linoleic acid in the cells incubated with Somatostatin+Quercetin and Quercetin and decrease of omega-3 acids in the cells incubated with Somatostatin+Curcumin compared to Som...
Source: Canadian Journal of Physiology and Pharmacology - September 23, 2019 Category: Drugs & Pharmacology Authors: Hanikoglu A, Kucuksayan E, Hanikoglu F, Ozben T, Menounou G, Sansone A, Chatgilialoglu C, Di Bella G, Ferreri C Tags: Can J Physiol Pharmacol Source Type: research

Vitamin d alleviates oxidative stress in adipose tissue and mesenteric vessels from obese patients with subclinical inflammation.
We report here that serum level of vitamin D inversely correlated with the magnitude of oxidative stress in the adipose tissue. Ex vivo treatment with active vitamin D mitigated obesity-related oxidative stress. PMID: 31545914 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 23, 2019 Category: Drugs & Pharmacology Authors: Ionica M, Aburel OM, Vaduva A, Petrus A, Rațiu S, Olariu S, Sturza A, Muntean DM Tags: Can J Physiol Pharmacol Source Type: research

The effects of gasotransmitters inhibition on biochemical and haematological parameters, and oxidative stress in propofol-anaesthetized wistar male rats.
This study aimed to investigate the effects of propofol through evaluating it`s interaction with nitric oxide - NO, hydrogen sulfide - H2S and carbon monoxide - CO. Wistar male rats were divided in 4 groups: 1) bolus injection of propofol (1% 10 mg/ml, 100 mg/kg bw, i.p.); 2) N(ω)-nitro-L-arginine methyl ester - L-NAME (NO synthase inhibitor, 60 mg/kg bw, i.p.)+bolus injection of propofol (1% 10 mg/ml, 100 mg/kg bw, i.p.); 3) DL-propargylglycine - DL-PAG (H2S synthase inhibitor, 50 mg/kg bw, i.p.)+bolus injection of propofol (1% 10 mg/ml, 100 mg/kg bw, i.p.); 4) Zinc protoporphyrin IX - ZnPPIX (CO synthase inhibitor,...
Source: Canadian Journal of Physiology and Pharmacology - September 19, 2019 Category: Drugs & Pharmacology Authors: Djuric M, Nikolic T, Radonjic K, Kostic S, Stankovic S, Zivkovic VI, Djuric D, Jakovljevic V, Stevanovic P Tags: Can J Physiol Pharmacol Source Type: research

A new insight into the molecular hydrogen effect on coenzyme Q and mitochondrial function of rats.
Abstract Mitochondria are the major source of cellular energy metabolism. In their function they produce a significant amount of free radicals, which in turn affects their function. Imbalance between ROS and antioxidant defense leads to oxidative stress. In this paper we demonstrate that the consumption of molecular hydrogen-rich water (HRW) resulted in stimulated rat cardiac mitochondrial electron respiratory chain function and increased levels of ATP production by Complex I and Complex II substrates. Similarly, coenzyme Q9 levels in rat plasma, myocardial tissue and mitochondria were increased and malondialdehyd...
Source: Canadian Journal of Physiology and Pharmacology - September 19, 2019 Category: Drugs & Pharmacology Authors: Gvozdjakova A, Kucharska J, Kura B, Vancova O, Rausova Z, Sumbalova Z, Ulicna O, Slezak J Tags: Can J Physiol Pharmacol Source Type: research

Human concentrations of uric acid scavenges adaptive and maladaptive ROS in isolated rat hearts subjected to ischemic stress.
Abstract Uric acid is a purine degradation product but also an important antioxidant and ROS scavenger. Experimental settings that mimic myocardial ischemia-reperfusion have not included uric acid despite that it is always present in human extracellular fluid and plasma. We hypothesized that uric acid has an important role in myocardial ROS scavenging. Here, we tested the cardiac response to uric acid on infarct size following ischemia-reperfusion with and without exacerbated oxidative stress due to acute pressure overload and during preconditioning. We also examined mitochondrial respiration and ROS-induced mitoc...
Source: Canadian Journal of Physiology and Pharmacology - September 13, 2019 Category: Drugs & Pharmacology Authors: Boardman NT, Falck AT, Lund T, Chu X, Martin-Armas M, Norvik JV, Jenssen TG, Ytrehus K Tags: Can J Physiol Pharmacol Source Type: research

Risk factors for anxiety and depression in Chinese patients undergoing surgery for endometrial cancer.
In conclusion, patients with endometrial cancer have a higher incidence of postoperative anxiety and depression where postoperative pain, liver disease and decreased immune function are risk factors for both anxiety and depression in these patients. PMID: 31518506 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 13, 2019 Category: Drugs & Pharmacology Authors: Wang B, Li B, Tan S, Zhai J, Chen M Tags: Can J Physiol Pharmacol Source Type: research

Retinoic acid attenuates cardiac injury induced by hyperglycemia in pre- and post-delivery mice.
In conclusion, RA treatment could increase the activity of the antioxidant enzyme, suppress the oxidative stress, inflammation response and activation of NF-κB signaling, thereby improving blood glucose level and cardiac injury of GDM mice before and after delivery. PMID: 31518508 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 13, 2019 Category: Drugs & Pharmacology Authors: Liu Y, Zhao J, Lu M, Wang H, Tang F Tags: Can J Physiol Pharmacol Source Type: research

Amelioration of estrogen-induced endometrial hyperplasia in female rats by hemin via heme-oxygenase-1 expression, suppression of iNOS, p38MAPK and Ki67.
In conclusion, upregulation of HO-1 expression via hemin has ameliorative effect against EH through its anti-oxidant, anti-inflammatory and anti-proliferative actions. PMID: 31505119 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 10, 2019 Category: Drugs & Pharmacology Authors: Ali FF, Abdelzaher W, Ibrahim RA, Elroby Ali D Tags: Can J Physiol Pharmacol Source Type: research

Modulation of oxidative/nitrosative stress and inflammatory response by rapamycin in target and distant organs in rats exposed to hindlimb ischemia/reperfusion: The role of mTOR.
The objective of the current study was to evaluate the role of the mTOR in oxidative/nitrosative stress and inflammation in hindlimb I/R-induced injury in target and remote organ injuries. In rats subjected to I/R, an increased expression of ribosomal protein S6 (rpS6), inhibitor κB (IκB)-α, nuclear factor-κB (NF-κB) p65, iNOS, cyclooxygenase (COX-2), gp91phox, and levels of tumor necrosis factor (TNF-α), nitrite, nitrotyrosine, malondialdehyde (MDA) and the activities of myeloperoxidase (MPO) and catalase in the tissues and/or sera were detected. Treatment with rapamycin, a selective in...
Source: Canadian Journal of Physiology and Pharmacology - September 10, 2019 Category: Drugs & Pharmacology Authors: Kocak Z, Temiz-Resitoglu M, Guden DS, Vezir O, Sucu N, Balci S, Tamer L, Tunctan B, Malik KU, Sahan-Firat S Tags: Can J Physiol Pharmacol Source Type: research

The influence of subchronic co-application of vitamins b6 and folic acid on cardiac oxidative stress and biochemical markers in monocrotaline-induced heart failure in male wistar albino rats.
In this study co-application of vitamins B6 and folic acid did not attenuate hypertrophy of right ventricle wall, but aggravated oxidative stress which is involved in HF pathogenesis. PMID: 31505124 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 10, 2019 Category: Drugs & Pharmacology Authors: Jakovljevic Uzelac J, Djukic T, Mutavdzin S, Stankovic S, Labudovic Borovic M, Rakočević J, Milic N, Savic Radojevic A, Vasić M, Japundzic Zigon N, Simic T, Djuric D Tags: Can J Physiol Pharmacol Source Type: research

Combined effects of exercise training and high doses of anabolic steroids on cardiac autonomic modulation and ventricular repolarization properties in rats.
In conclusion, AAS produced cardiac autonomic dysfunction and ventricular repolarization disturbances in rats. Combining an exercise training protocol during the AAS treatment attenuated the ventricular repolarization abnormalities and did not prevent cardiac autonomic dysfunction. PMID: 31505126 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - September 10, 2019 Category: Drugs & Pharmacology Authors: Marocolo M, Katayama PL, Meireles A, Barbosa Neto O Tags: Can J Physiol Pharmacol Source Type: research

Vascular dysfunction following breath-hold diving.
This study focused on vascular damage and its potential role in the genesis of DCS in breath-hold diving. Eleven breath-hold divers participated in a field study comprising eight deep breath-hold dives with short surface periods, and repetitive breath-hold dives lasting for six hours. Endothelium-dependent vasodilation of the brachial artery, via flow-mediated dilation (FMD), and the number of microparticles (MPs) were assessed before and after each protocol. All measures were analyzed by two-way within-subject analysis of variance (2x2 ANOVA; factors: time and protocol). Absolute FMD was reduced following both diving protocols (p
Source: Canadian Journal of Physiology and Pharmacology - September 10, 2019 Category: Drugs & Pharmacology Authors: Barak OF, Janjic N, Drvis I, Mijacika T, Mudnic I, Coombs GB, Thom SR, Madic D, Dujic Z Tags: Can J Physiol Pharmacol Source Type: research

The effect dioxidovanadium complex (v) on hepatic function in streptozotocin-induced diabetic rats.
Abstract Diabetics are susceptible hepatic dysfunction risks due to hyperglycaemia and insulin therapy. Diabetes conventional treatments improve glycaemic control, however hepatic hazards associated with these agents remains a challenge. Accordingly, this study sought to investigate the effect of a dioxidovanadium complex (V) on the hepatic function in STZ-induced diabetic rats. Sprague-Dawley rats (240-250g) were divided into 4 groups (n=6) namely, non-diabetic control, diabetic control, insulin treated and vanadium complex group. The dioxidovanadium (10, 20, 40 mg/kg) was administered twice every 2nd day for 5 w...
Source: Canadian Journal of Physiology and Pharmacology - September 6, 2019 Category: Drugs & Pharmacology Authors: Sibiya S, Msibi B, Khathi A, Sibiya N, Booysen I, Ngubane P Tags: Can J Physiol Pharmacol Source Type: research

Adverse plasma fatty acid composition in patients with femoral neck fracture.
Abstract Our study was aimed to examine the status of plasma fatty acids (FAs), inflammatory markers and lipid peroxidation in patients with femoral neck fractures. Study included 20 patients (64-86 years) with femoral neck fractures, indicated for surgery, and control group of 17 elderly subjects, without fractures or serious chronic diseases. Plasma was obtained during the first 12 hours postfracture and presurgery, and 7 days postop. Compared to control, patients had significantly higher saturated FA (SFA) and monounsaturated FA, as well as increased TNF-α and IL-6. In opposite, levels of individual and t...
Source: Canadian Journal of Physiology and Pharmacology - September 6, 2019 Category: Drugs & Pharmacology Authors: Arsic A, Pesic G, Petrovic S, Matic A, Jeremic J, Jakovljevic V, Vucic V Tags: Can J Physiol Pharmacol Source Type: research

3-Bromopyruvate potentiates TRAIL-induced apoptosis in human colon cancer cells through a reactive oxygen species- and caspase-dependent mitochondrial pathway.
This study aimed to investigate the inhibitory effect of co-treatment of 3-Bromopyruvate (3-BP) as a potent anticancer agent with TRAIL on colon cancer cells (HT-29). The results of present study indicated that combined treatment with 3-BP and TRAIL inhibited the proliferation of HT-29 cells to a greater extent (88.4%) compared with 3-BP (54%) or TRAIL (11%) treatment alone. In contrast, the combination of 3-BP and TRAIL had no significant inhibitory effect on the proliferation of normal cells (HEK-293) (8.4%). At a cellular mechanistic level, the present study showed that 3-BP sensitized human colon cancer cells to TRAIL-...
Source: Canadian Journal of Physiology and Pharmacology - September 6, 2019 Category: Drugs & Pharmacology Authors: Abbaszadeh H, Valizadeh A, Mahdavinia M, Teimoori A, Pipelzadeh MH, Zeidooni L, Alboghobeish S Tags: Can J Physiol Pharmacol Source Type: research

Special issue of selected papers from the 5th European Section Meeting of the International Academy of Cardiovascular Sciences held in Smolenice, Slovakia, on 23-26 May 2018.
Authors: PMID: 31448966 [PubMed - in process] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - August 27, 2019 Category: Drugs & Pharmacology Tags: Can J Physiol Pharmacol Source Type: research

Introduction.
PMID: 31448967 [PubMed - in process] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - August 27, 2019 Category: Drugs & Pharmacology Authors: Slezak J, Ravingerova T, Djuric D Tags: Can J Physiol Pharmacol Source Type: research

Inhibition of microRNA-34a mediates protection of thymosin beta 4 in endothelial progenitor cells against advanced glycation endproducts by targeting B-cell lymphoma 2.
In conclusion, inhibition of microRNA-34a mediated protection of thymosin beta 4 in endothelial progenitor cells against advanced glycation endproducts by targeting B-cell lymphoma 2, which was helpful for understanding the therapeutic potential of thymosin beta 4 for diabetic patients. PMID: 31397599 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - August 9, 2019 Category: Drugs & Pharmacology Authors: Chen Q, Shen Z, Mao Y, Li Q, Liu Y, Mei M, Qiu F, Wang M Tags: Can J Physiol Pharmacol Source Type: research

Contribution of CYP27B1 and CYP24A1 Genetic Variations to the Incidence of Acute Coronary Syndrome and to Vitamin D Serum Level.
This study aims at investigating the association between single nucleotide polymorphisms (SNPs) in genes involved in vitamin D metabolism; CYP27B and CYP24A1; 25(OH)D levels, and susceptibility to acute coronary syndrome (ACS). SUBJECTS/METHODS: 185 patients and 138 healthy controls were recruited. CYP24A1 rs2762939 was genotyped using Fast Real time PCR, while CYP24A1 rs4809960 and CYP27B1 rs703842 were genotyped using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP). 25(OH)D3and 25(OH)D2 levels were measured using ultra-performance liquid chromatography tandem mass spectrum (...
Source: Canadian Journal of Physiology and Pharmacology - August 9, 2019 Category: Drugs & Pharmacology Authors: Fam MS, Hassanein SI, Abdel Rahman MF, Assal RA, Hanafi RS, Gad MZ Tags: Can J Physiol Pharmacol Source Type: research

Resveratrol prevents palmitic acid induced cardiomyocyte contractile impairment.
In conclusion, our results show that Resv protect cardiomyocytes from contractile dysfunction induced by PA. PMID: 31374178 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - August 2, 2019 Category: Drugs & Pharmacology Authors: Lieben Louis X, Raj P, Meikle Z, Yu L, Susser S, MacInnis S, Duhamel TA, Wigle JT, Netticadan T Tags: Can J Physiol Pharmacol Source Type: research

Genetics of hyperhomocysteinemia and 1-carbon metabolism: implications for retinal structure and eye functions.
Abstract Homocysteine (Hcy); a sulfur-containing non-proteinogenic amino acid is generated as a metabolic intermediate. Hcy constitutes an important part of the "1-carbon metabolism" during methionine turnover. Elevated levels of Hcy known as hyperhomocysteinemia (HHcy) results from vitamin B deficiency, lack of exercise, smoking, excessive alcohol intake, high fat and methionine rich diet, and the underlying genetic defects. These factors directly affect the "1-carbon metabolism (methionine-Hcy-folate)" of a given cell. In fact, the Hcy levels are determined primarily by dietary intake, vitami...
Source: Canadian Journal of Physiology and Pharmacology - August 1, 2019 Category: Drugs & Pharmacology Authors: George AK, Majumder A, Ice H, Homme RP, Eyob W, Tyagi SC, Singh M Tags: Can J Physiol Pharmacol Source Type: research

Lycopene improves methotrexate-induced functional alterations of the Madin-Darby kidney cells in a concentration-dependent manner.
In this study we evaluated effects of lycopene on the Madin-Darby canine kidney cells (MDCK)treated with methotrexate through the estimation of their mitochondrial and lysosomal functions (MTT reduction assay and NR uptake assay) and changes in cell oxidative status (determination of advanced oxidized proteins concentrations and reduced glutathione levels) and lysosomal enzymes activity (β-N acetyl glucosaminidase activity).Results of our study showed that lycopene applied in high concentration caused significant impairment of the MDCK function leading to cell death. Contrary, in relatively low concentrations lycopene...
Source: Canadian Journal of Physiology and Pharmacology - August 1, 2019 Category: Drugs & Pharmacology Authors: Stojiljkovic N, Ilic S, Stojanovic N, Stojanovic S, Stoiljkovic M Tags: Can J Physiol Pharmacol Source Type: research

Role of Lysophosphatidic Acid in Vascular Smooth Muscle Cell Proliferation.
Abstract Lysophosphatidic acid (LPA) is an important lipid molecule for signal transduction in cell proliferation. Although the effects of LPA on vascular smooth muscle (VSM) cell growth have been reported previously, the underlying mechanisms of its action are not fully understood. The present study was undertaken to investigate the effects of some inhibitors of different protein kinases and other molecular targets on LPA-induced DNA synthesis as well as gene expression in the aortic VSM cells. The DNA synthesis was studied by [3H]-thymidine incorporation method and the gene expression was investigated by real ti...
Source: Canadian Journal of Physiology and Pharmacology - August 1, 2019 Category: Drugs & Pharmacology Authors: Liu Y, Chen F, Ji L, Zhang L, Xu YJ, Dhalla NS Tags: Can J Physiol Pharmacol Source Type: research

Evaluation of 3-day azithromycin or 5-day cefaclor in comparison with 10-day amoxicillin for treatment of tonsillitis in children.
Abstract To evaluate the clinical efficacy of azithromycin, cefaclor, and amoxicillin in treatment of pediatric tonsillitis, a total of 256 children with Group A β-hemolytic streptococcus (GAS) tonsillitis were randomly divided into 3 groups. Only patients assessed with streptococcus-positive tonsillitis, considered to be compliant with treatment and complete clinical and microbiological evaluations at the end of therapy (day 14) and follow-up (day 30) were included in the efficacy analysis. Our study demonstrated that 96.4% of patients in the azithromycin group, 92.4% of patients in the cefaclor group, and 9...
Source: Canadian Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Authors: Li P, Jiang G, Shen X Tags: Can J Physiol Pharmacol Source Type: research

Blueberry extract attenuates doxorubicin-induced damage in H9c2 cardiac cells 1.
The objective of this study was to analyze the cardioprotective roles of 3 wild blueberry genotypes and one commercial blueberry genotype by measuring markers of oxidative stress and cell death in H9c2 cardiac cells exposed to doxorubicin. Ripe berries of the 3 wild blueberry genotypes were collected from a 10-year-old clearcut forest near Nipigon, Ontario, Canada (49°1'39″N, 87°52'21″W), whereas the commercial blueberries were purchased from a local grocery store. H9c2 cardiac cells were incubated with 15 μg gallic acid equivalent/mL blueberry extract for 4 h followed by 5 μM doxorubicin for 4 h,...
Source: Canadian Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Authors: Sun Y, Nemec-Bakk AS, Mallik AU, Bagchi AK, Singal PK, Khaper N Tags: Can J Physiol Pharmacol Source Type: research

Angiotensin II type 2 receptor blocker PD123319 has more beneficial effects than losartan on ischemia/reperfusion injury and oxidative damage in isolated rat heart.
Abstract Our study aimed to determine the effects of Losartan and PD123319 in ischemia/reperfusion (IR) injury in isolated perfused rat heart. The study used 40 male Wistar albino rats that were grouped as Control, IR, and IR groups which received Losartan (20 mg/kg), PD123319 (20 mg/kg) and Losartan+PD123319. The hearts were attached to Langendorff isolated heart system by employing in situ cannulation method, and cardiodynamic parameters were recorded during the experiment. At the end of experiment hearts were retained for biochemical analysis, and all data were statistically evaluated. A partial recovery of car...
Source: Canadian Journal of Physiology and Pharmacology - July 30, 2019 Category: Drugs & Pharmacology Authors: Kilic A, Ustunova S, Usta C, Bulut H, Meral I, Demirci Tansel C, Gurel Gurevin E Tags: Can J Physiol Pharmacol Source Type: research

The approximate formulas predicting personal somatic telomere length using patient blood test data.
This study analyzed the correlation between telomere length in peripheral leukocytes and laboratory data to select test items relating closely to biological aging. We established formulas from these clinical data to predict the personal telomere length. The subjects were patients having visited Kyushu University Beppu Hospital from 2012 to 2015. Two hundred thirty-two patients were enrolled. The blood data were collected and telomere lengths were measured by Southern blotting method. The patients showed significant correlations between the telomere length and several blood test data with a gender-related difference. Candid...
Source: Canadian Journal of Physiology and Pharmacology - July 24, 2019 Category: Drugs & Pharmacology Authors: Maeda T, Horiuchi T, Makino N Tags: Can J Physiol Pharmacol Source Type: research

Effects of Resveratrol Postconditioning on Cerebral Ischemia in mice: Role of the Sirtuin-1 (SIRT1) Pathway.
Abstract Evidence has demonstrated that resveratrol preconditioning exhibits neuroprotection against cerebral IR injury. The current investigation aimed to explore whether pharmacological postconditioning, by administering resveratrol, after a sustained ischemia & prior to prolonged reperfusion abrogates cerebral IR injury. Cerebral ischemia-reperfusion-induced injury mice model was employed in this study to evaluate the neuroprotective effects of pharmacological postconditioning (pPoCo) with resveratrol (30 mg/kg; i.p.) administered 5 mins before reperfusion. We administered Sirtinol, a SIRT1/2 selective inhi...
Source: Canadian Journal of Physiology and Pharmacology - July 24, 2019 Category: Drugs & Pharmacology Authors: Grewal AK, Singh N, Singh TGT Tags: Can J Physiol Pharmacol Source Type: research

Investigation of intestinal elimination and biliary excretion of ibuprofen in control and hyperglycemic rats.
esi P Abstract An in vivo intestinal perfusion model was used to investigate how experimental hyperglycemia affects intestinal elimination and biliary excretion in the rat. Experimental diabetes was induced by administration of streptozotocin (65mg/kg i.v.). The intestinal perfusion medium contained 250 µM (±)-ibuprofen. An isocratic HPLC method with UV-Vis detection was developed to quantitate ibuprofen in the intestinal perfusate and a gradient method was applied to quantitate ibuprofen and ibuprofen-β-D-glucuronide in the bile. The limit of quantitation of ibuprofen was found to be 0.51 µ...
Source: Canadian Journal of Physiology and Pharmacology - July 24, 2019 Category: Drugs & Pharmacology Authors: Kovacs NP, Almási A, Garai K, Kuzma M, Vancea S, Fischer E, Perjesi P Tags: Can J Physiol Pharmacol Source Type: research

Chronic ethanol consumption induces micturition dysfunction and alters the oxidative state of the urinary bladder.
Abstract Oxidative stress is pointed out as a major mechanism by which ethanol induces functional and structural changes in distinctive tissues. We evaluated whether ethanol consumption would increase oxidative stress and cause/span>micturition dysfunction. Male C57BL/6J mice were treated with ethanol 20% (vol./vol.) for 10 weeks. Our findings showed that chronic ethanol consumption reduced micturition spots and urinary volume in conscious mice, whereas in anaesthetized animals cystometric analysis revealed reduced basal pressure and increased capacity, threshold pressure and maximum voiding. Treatment with eth...
Source: Canadian Journal of Physiology and Pharmacology - July 24, 2019 Category: Drugs & Pharmacology Authors: Do Vale GT, Sousa AH, Gonzaga NA, De Oliveira MG, Justo AFO, Alexandre EC, Tanus-Santos JE, Antunes E, Tirapelli CR Tags: Can J Physiol Pharmacol Source Type: research

Oral ethinylestradiol-levonorgestrel normalizes fructose-induced hepatic lipid accumulation and glycogen depletion in female rats.
Abstract The present study investigated effects of oral ethinylestradiol-levonorgestrel (EEL) on hepatic lipid and glycogen contents during high fructose (HF) intake and determined whether pyruvate dehydrogenase kinase-4 (PDK-4) and Glucose-6-phosphate dehydrogenase (G6PD) activity were involved in HF and/or EEL-induced hepatic dysmetabolism. Female Wistar rats weighing between 140-160g were divided into groups. Control, EEL, HF and EEL+HF groups received water (vehicle, p.o), 1.0µg ethinylestradiol plus 5.0µg levonorgestrel (p.o), fructose (10%; w/v) and EEL plus HF respectively, daily for 8 weeks. Re...
Source: Canadian Journal of Physiology and Pharmacology - July 23, 2019 Category: Drugs & Pharmacology Authors: Olaniyi KS, Olatunji LA Tags: Can J Physiol Pharmacol Source Type: research

Role of Brd4 in the production of inflammatory cytokines in mouse macrophages treated with titanium particles.
In conclusion, Brd4 expression increases in interface membrane and Brd4 participates in the production of pro-inflammatory cytokines induced by Ti particles via promoting the activation of NF-B signaling and binding to acetylated NF-κB p65 (lysine-310) in mouse macrophages. PMID: 31330113 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 22, 2019 Category: Drugs & Pharmacology Authors: Ren Y, Zhang Y, Wang Z, Wang C, Zhang H, Wang Y, Zhao Z Tags: Can J Physiol Pharmacol Source Type: research

The Contribution of Phosphodiesterases to Cardiac Dysfunction in High Carbohydrate Diet-Induced Metabolic Syndrome Rats.
Abstract Metabolic syndrome (MetS) is a cluster of risk factors such as insulin resistance among others, underlying the development of diabetes and/or cardiovascular diseases. Studies show a close relationship between cardiac dysfunction and abnormal cAMP catabolism, contributing to pathological-remodeling. Taken into consideration the importance of therapeutic enhancement of cAMP by stimulating its synthesis via suppression of PDEs, we examined their roles on cardiac dysfunction in high carbohydrate diet-induced MetS-rats. We first demonstrated significantly high expression levels of PDE3 and PDE4, the most highl...
Source: Canadian Journal of Physiology and Pharmacology - July 12, 2019 Category: Drugs & Pharmacology Authors: Okatan EN, Turan B Tags: Can J Physiol Pharmacol Source Type: research

The Effects of Folic Acid Administration on Cardiac Tissue Matrix Metalloproteinases Activities and Hepatorenal Biomarkers in Diabetic Rats.
In conclusion, folic acid treatment of diabetic rats has cardio- and hepatoprotective effects. Nevertheless, folic acid dosing should be carefully considered because of possible renal damage. PMID: 31295411 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 11, 2019 Category: Drugs & Pharmacology Authors: Mutavdzin S, Gopcevic K, Stankovic S, Jakovljevic Uzelac J, Labudovic Borovic M, Djuric D Tags: Can J Physiol Pharmacol Source Type: research

Molecular hydrogen suppresses free radical-induced cell death by mitigating fatty acid peroxidation and mitochondrial dysfunction.
Abstract Molecular hydrogen (H2) was believed to be an inert and non-functional molecule in mammalian cells; however, we overturned the concept by reporting the therapeutic effects of H2 against oxidative stress. Subsequently, extensive studies revealed multiple functions of H2 by exhibiting the efficacies of H2 in various animal models and clinical studies. Here, we investigated the effect of H2 on free radical-induced cytotoxicity using tert-butyl hydroperoxide (TBHP) in a human acute monocytic leukemia cell line, THP-1. Cell membrane permeability was determined using lactate dehydrogenase (LDH) release assay an...
Source: Canadian Journal of Physiology and Pharmacology - July 11, 2019 Category: Drugs & Pharmacology Authors: Iuchi K, Nishimaki K, Kamimura N, Ohta S Tags: Can J Physiol Pharmacol Source Type: research

Endocannabinoid and N-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration.
Abstract Contribution of cannabinoid system has been suspected to play role in the mechanisms of actions of dipyrone and paracetamol. Our purpose was to measure the local endocannabinoid and N-acylethanolamide levels in brain and spinal cord of rats following dipyrone and paracetamol administration. In this project, nociception tests were assessed 1-, 5- and 12-hours following drug injections in Wistar rats, using tail-flick and hot-plate apparatus. Firstly, antinociceptive effects of dipyrone (150, 300, 600 mg/kg, i.p.) and paracetamol (30, 100, 300 mg/kg, i.p.) are observed. Then, after administration of highest...
Source: Canadian Journal of Physiology and Pharmacology - July 8, 2019 Category: Drugs & Pharmacology Authors: Topuz RD, Gunduz O, Karadag HC, Dokmeci D, Ulugol A Tags: Can J Physiol Pharmacol Source Type: research

Endothelial and left ventricular diastolic function in young adults exposed to tobacco.
Abstract Smoking is associated to endothelial and left ventricular diastolic disfunction. We aimed to determine the endothelial and diastolic function in young adults exposed to tobacco smoke and the effects of acute exposure to it. Smokers were considered as cases and non smokers as controls. Brachial artery diameter, brachial artery flow velocity and echocardiographic variables were measured. Average age of the participants was 21 years old. Smokers showed significant endothelial dysfunction compared to non-smokers. Arterial dilatation mediated by the endothelium was significantly higher in non-smokers than in s...
Source: Canadian Journal of Physiology and Pharmacology - July 3, 2019 Category: Drugs & Pharmacology Authors: Rodriguez-Portelles A, Rodriguez Leyva D Tags: Can J Physiol Pharmacol Source Type: research

Dysregulation of 1-carbon metabolism and muscle atrophy: potential roles of forkhead box O proteins and PPAR γ co-activator-1α.
Dysregulation of 1-carbon metabolism and muscle atrophy: potential roles of forkhead box O proteins and PPARγ co-activator-1α. Can J Physiol Pharmacol. 2019 Jul 03;: Authors: Laha A, Singh M, George AK, Homme RP, Tyagi SC Abstract Homocysteine, a non-proteinogenic amino acid but an important metabolic intermediate is generated as an integral component for the "1-carbon metabolism" during normal physiology. It is catabolized to cysteine via the transulfuration pathway resulting in the generation of hydrogen sulfide, a naturally endogenous byproduct. Genetics or metabolic derangeme...
Source: Canadian Journal of Physiology and Pharmacology - July 3, 2019 Category: Drugs & Pharmacology Authors: Laha A, Singh M, George AK, Homme RP, Tyagi SC Tags: Can J Physiol Pharmacol Source Type: research

Consequences of low-intensity light at night on cardiovascular and metabolic parameters in spontaneously hypertensive rats.
Abstract Circadian rhythms are an inherent property of physiological processes and can be disturbed by irregular environmental cycles, including artificial light at night (ALAN). Circadian disruption may contribute to many pathologies, such as hypertension, obesity and type 2 diabetes, but the underlying mechanisms are not understood. Our study investigated the consequences of ALAN on cardiovascular and metabolic parameters in spontaneously hypertensive rats (SHR), which represent an animal model of essential hypertension and insulin resistance. Adult males were exposed to a light (L)/dark (D) cycle of 12:12 h and...
Source: Canadian Journal of Physiology and Pharmacology - June 28, 2019 Category: Drugs & Pharmacology Authors: Rumanova VS, Okuliarova M, Molcan L, Sutovska H, Zeman M Tags: Can J Physiol Pharmacol Source Type: research

Drinking Hydrogen Water Enhances Endurance and Relieves Psychometric Fatigue: Randomized, Double-blind, Placebo-controlled Study.
Abstract Acute physical exercise increases reactive oxygen species in skeletal muscle, leading to tissue damage and fatigue. Molecular hydrogen (H2) acts as a therapeutic antioxidant directly or indirectly by inducing anti-oxidative enzymes. Here, we examined the effects of drinking H2 water (H2-infused water) on psychometric fatigue and endurance capacity in a randomized, double-blind, placebo-controlled fashion. In experiment 1 all participants drank only placebo water in the first cycle ergometer exercise session, and for comparison they drank either H2 water or placebo water 30 min before exercise in the secon...
Source: Canadian Journal of Physiology and Pharmacology - June 28, 2019 Category: Drugs & Pharmacology Authors: Mikami T, Tano K, Lee H, Lee H, Park J, Ohta F, LeBaron TW, Ohta S Tags: Can J Physiol Pharmacol Source Type: research

Increased systolic blood pressure associated with hypertriglyceridemia in female Sprague Dawley rats.
In conclusion, hypertriglyceridemia may have increased resistance arteries responsiveness to alpha-agonist and systolic BP in female rats. PMID: 31247146 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 27, 2019 Category: Drugs & Pharmacology Authors: Mogane C, Mokotedi L, Millen AM, Michel FS Tags: Can J Physiol Pharmacol Source Type: research

ARHGAP21-deficiency impairs hepatic lipid metabolism and improves insulin signaling in lean and obese mice.
In conclusion, liver of ARHGAP21-haplodeficient mice are more insulin sensitive, associated with higher lipid synthesis and lower lipid export. PMID: 31247150 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 27, 2019 Category: Drugs & Pharmacology Authors: Zangerolamo L, Soares GM, Vettorazzi JF, Amaral MEC, Carneiro EM, Olalla Saad ST, Boschero AC, Barbosa-Sampaio HCL Tags: Can J Physiol Pharmacol Source Type: research

Exercise-induced increases in the expression and activity of cardiac sarcoplasmic reticulum calcium ATPase 2 is attenuated in AMPK α2 kinase-dead mice.
Exercise-induced increases in the expression and activity of cardiac sarcoplasmic reticulum calcium ATPase 2 is attenuated in AMPKα2 kinase-dead mice. Can J Physiol Pharmacol. 2019 Jun 25;:1-10 Authors: Morissette MP, Susser SE, Stammers AN, Moffatt TL, Wigle JT, Wigle TJ, Netticadan T, Premecz S, Jassal DS, O'Hara KA, Duhamel TA Abstract Exercise enhances cardiac sarcoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) function through unknown mechanisms. The present study tested the hypothesis that the positive effects of exercise on SERCA2a expression and function in the left ventricle is dependent on...
Source: Canadian Journal of Physiology and Pharmacology - June 25, 2019 Category: Drugs & Pharmacology Authors: Morissette MP, Susser SE, Stammers AN, Moffatt TL, Wigle JT, Wigle TJ, Netticadan T, Premecz S, Jassal DS, O'Hara KA, Duhamel TA Tags: Can J Physiol Pharmacol Source Type: research

Decreased liver triglyceride content in adult rats exposed to protein restriction during gestation and lactation: Roles of hepatic lipogenesis and lipid utilization in muscle and adipose tissue.
Abstract Protein restriction throughout pregnancy and lactation reduces liver triglyceride (TG) content in adult male rat offspring. The study determined the contribution of hepatic lipogenesis to the reduction in liver TG content. Rats received either control or protein restricted diets throughout pregnancy and lactation. Offspring were sacrificed on day 65. Hepatic fatty acid uptake and de novo fatty acid and triglyceride biosynthesis were similar between control and low protein offspring (LP). These results indicate that hepatic lipogenesis cannot mediate the decrease in liver TG content in LP offspring. We the...
Source: Canadian Journal of Physiology and Pharmacology - June 25, 2019 Category: Drugs & Pharmacology Authors: Mendez-Garcia C, Trini A, Browne V, Kochansky CJ, Pontiggia L, D'mello A Tags: Can J Physiol Pharmacol Source Type: research

Cardioprotective effect of ranolazine in nondiabetic and diabetic male rats subjected to isoprenaline-induced acute myocardial infarction involves modulation of AMPK and inhibition of apoptosis.
Abstract Diabetes increases the sensitivity of myocardium to ischemic damage and impairs response of the myocardium to cardioprotective interventions. The present study aimed to elucidate the potential cardioprotective effect provided by ranolazine during myocardial infarction in nondiabetic and diabetic male rats. As AMP-activated protein kinase (AMPK) has been shown to be involved in the cellular response to ischemic injury, in this context, the present animal study evaluated the modulating role of ranolazine in the AMPK expression in isoprenaline-induced myocardial ischemic rat model. Male rats were divided int...
Source: Canadian Journal of Physiology and Pharmacology - June 3, 2019 Category: Drugs & Pharmacology Authors: Tawfik MK, Ameen AM Tags: Can J Physiol Pharmacol Source Type: research