Correction: Elevated aldolase 1A, retrogene 1 expression induces cardiac apoptosis in rat experimental autoimmune myocarditis model.
PMID: 32744871 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - August 3, 2020 Category: Drugs & Pharmacology Authors: Choi S, Chung JH, Nam MH, Bang E, Hong KS, Kim YH, Seo JB, Chi SG Tags: Can J Physiol Pharmacol Source Type: research

Endothelin receptor heteromerization inhibits β-arrestin function in HEK293 cells.
Endothelin receptor heteromerization inhibits β-arrestin function in HEK293 cells. Can J Physiol Pharmacol. 2020 Aug 03;:531-540 Authors: Zrein A, Bagher AM, Young AP, Denovan-Wright EM, Kelly MEM Abstract The endothelin receptor A (ETA) and endothelin receptor B (ETB) are G protein-coupled receptors that are co-expressed in vascular smooth muscle cells. Endothelin-1 (ET-1) activates endothelin receptors to cause microvascular vasoconstriction. Previous studies have shown that heteromerization between ETA and ETB prolongs Ca2+ transients, leading to prolongation of Gαq-dependent signaling a...
Source: Canadian Journal of Physiology and Pharmacology - August 3, 2020 Category: Drugs & Pharmacology Authors: Zrein A, Bagher AM, Young AP, Denovan-Wright EM, Kelly MEM Tags: Can J Physiol Pharmacol Source Type: research

miR-138-5p targets sirtuin1 to regulate acute lung injury by regulation of the NF- κB signaling pathway.
In this study, we explored the mechanism of the occurrence and development of ALI. It was firstly found that miR-138-5p could inhibit the expression of sirtuin1 (SIRT1), and we further demonstrated that miR-138-5p targets directly SIRT1 through the luciferase assay, while the latter negatively regulated the expression of NF-κB. A549 cells were treated with lipopolysaccharide in vitro to simulate ALI cells and induce ALI in the model mice. The results showed that inhibiting the expression of miR-138-5p could effectively increase the viability of damaged cells, promote cell proliferation, reduce apoptosis, inhibit the ...
Source: Canadian Journal of Physiology and Pharmacology - July 30, 2020 Category: Drugs & Pharmacology Authors: Wu Y, Jiang W, Lu Z, Su W, Liu N, Guo F Tags: Can J Physiol Pharmacol Source Type: research

Hepatoprotective effect of Linagliptin against liver fibrosis induced by carbon tetrachloride in mice.
In conclusion, linagliptin might help to protect the liver against persistent injury-related consequences. PMID: 32726558 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 29, 2020 Category: Drugs & Pharmacology Authors: Abd Elmaaboud M, Khattab H, Shalaby S Tags: Can J Physiol Pharmacol Source Type: research

p75NTR silencing inhibits proliferation, migration and extracellular matrix deposition of hypertrophic scar fibroblasts by activating autophagy through inhibiting PI3K/Akt/mTOR pathway.
Abstract Hypertrophic scar (HS) results from abnormal wound healing, accompanied by excessive hypercellularity, migration and extracellular matrix (ECM) deposition. Autophagy dysregulation plays crucial roles during HS formation. The overexpressed p75 neurotrophin receptor (p75NTR) in injured skin tissue after wound healing becomes a factor aggravating scar. The study was designed to investigate the role of p75NTR and p75NTR-mediated autophagy in the process of HS. The results revealed that p75NTR expression was significantly upregulated while that of autophagy proteins was downregulated in cicatrix at 3 and 6 mon...
Source: Canadian Journal of Physiology and Pharmacology - July 29, 2020 Category: Drugs & Pharmacology Authors: Shi W, Wu Y, Bian D Tags: Can J Physiol Pharmacol Source Type: research

Antifibrotic Effects of Bezafibrate and Pioglitazone Against Thioacetamide-Induced Liver Fibrosis in Albino Rats.
Abstract Activation of hepatic stellate cells is a central event in hepatic fibrogenesis that offers multiple potential sites for therapeutic interventions. Peroxisome proliferators activated receptors are implicated in liver fibrosis. We aimed to evaluate the effect of bezafibrate and pioglitazone on a thioacetamide (TAA) rat model of liver fibrosis and to clarify the possible underlying mechanisms. Rats received intraperitoneal injections of TAA for 6 weeks. Daily oral treatments with bezafibrate or pioglitazone were started with the first day of TAA-intoxication. Serum liver function tests, hepatic malondialdeh...
Source: Canadian Journal of Physiology and Pharmacology - July 28, 2020 Category: Drugs & Pharmacology Authors: Ibrahim S, Mohamed MZ, El-Tahway N, Abdelrahman AM Tags: Can J Physiol Pharmacol Source Type: research

Characterization of primary adult mouse cardiac fibroblast cultures.
Abstract The role of cardiac fibroblasts (CFs) in disease states has been a focus of cardiovascular research over the past decade. Here, we briefly describe methods for isolation and characterization of CFs from adult mouse ventricles. Primary cultures were stained using antibodies for several marker proteins such as alpha smooth muscle actin (aSMA), vimentin and Discoidin Domain Receptor 2 (DDR2) to confirm the identity of CFs or cardiac myofibroblasts (CMFs). Most cells in primary cultures consisted of CFs, with very low frequencies of endothelial cells, cardiomyocytes and smooth muscle cells. We compared marker...
Source: Canadian Journal of Physiology and Pharmacology - July 28, 2020 Category: Drugs & Pharmacology Authors: MacLean J, Pasumarthi KBS Tags: Can J Physiol Pharmacol Source Type: research

Hyperhomocysteinemia: an instigating factor for periodontal disease.
This study aims to investigate a HHcy mouse model (cystathionine β-synthase deficient; CBS+/-) for studying the potential pathophysiological changes, if any, in the periodontium (gingiva, periodontal ligament, cement and alveolar bone). We compared periodontium, side-by-side; CBS+/- model with that of wild-type; WT (C57BL/6J) mice. Histology and histomorphometry of mandibular bone along with gene expression analyses were carried out. Also, pro-angiogenic proteins and metalloproteinases were studied. To our knowledge, this research shows, for the first time, a direct connection between periodontal disease during CBS de...
Source: Canadian Journal of Physiology and Pharmacology - July 28, 2020 Category: Drugs & Pharmacology Authors: Stanisic D, George AK, Smolenkova I, Tyagi SC, Singh M Tags: Can J Physiol Pharmacol Source Type: research

Synergistic effect of Aminoguanidine and L-Carnosine against Thioacetamide-induced Hepatic Encephalopathy in rats: Behavioral, Biochemical and Ultra Structural Evidences.
This study was aimed to evaluate the possible synergistic effect between aminoguanidine (AG; 100 mg/kg; p.o.) and l-carnosine (CAR; 100 mg/kg; p.o.) on HE that was induced by thioacetamide (TAA; 100 mg/kg; i.p) thrice weekly for six weeks. Twenty-four hours after the last treatment; behavioral changes, biochemical parameters, histopathological analysis, immunohistochemical and ultrastructural studies were conducted. Combining AG with CAR improved TAA-induced locomotor impairment and motor incoordination evidenced by; reduced locomotor activity and decline in motor skill performance as well as ameliorated cognitive deficits...
Source: Canadian Journal of Physiology and Pharmacology - July 28, 2020 Category: Drugs & Pharmacology Authors: Afifi NA, Ramadan A, Erian E, Sedik AA, Amin MM, Hassan A, Saleh DO Tags: Can J Physiol Pharmacol Source Type: research

Protecting the aging eye with hydrogen sulfide.
Abstract Research demonstrates that senescence is associated with tissue and organ dysfunction, and the eye is no exception. Sequelae arising from aging have been well-defined as distinct clinical entities and vision-impairment has significant psychosocial consequences. Retina and adjacent tissues like retinal pigmented epithelium (RPE) and choroid are the key structures that are required for visual perception. Any structural and functional changes in retinal layers and blood retinal barrier (BRB) could lead to age-related macular degeneration (AMD), diabetic retinopathy (DR), and glaucoma. Further, there are sign...
Source: Canadian Journal of Physiology and Pharmacology - July 28, 2020 Category: Drugs & Pharmacology Authors: George AK, Homme RP, Stanisic D, Tyagi SC, Singh M Tags: Can J Physiol Pharmacol Source Type: research

Gut microbiota and the periodontal disease: role of hyperhomocysteinemia.
Abstract Periodontal disease results from poor oral hygiene and is characterized by a destructive process in periodontium that includes gingiva, alveolar mucosa, cementum, periodontal ligament and alveolar bone. Notably, destructive event in alveolar bone has been linked to homocysteine (Hcy) metabolism however; it has not been fully investigated. Therefore; implication of Hcy towards initiation, progression and maintenance of periodontal disease remains incompletely understood. Higher Hcy (or hyperhomocysteinemia; HHcy) exerts deleterious effects on gum health and teeth. Firstly, increased proinflammatory cytokin...
Source: Canadian Journal of Physiology and Pharmacology - July 24, 2020 Category: Drugs & Pharmacology Authors: Stanisic D, Jovanovic M, George AK, Homme RP, Tyagi N, Tyagi SC, Singh M Tags: Can J Physiol Pharmacol Source Type: research

Differential regulation of TGF β type-I receptor expressions in TGFβ1-induced myofibroblast differentiation.
Differential regulation of TGFβ type-I receptor expressions in TGFβ1-induced myofibroblast differentiation. Can J Physiol Pharmacol. 2020 Jul 23;: Authors: Gah A, Adil MS, Sabbineni H, Verma A, Somanath PR Abstract Fibroblast-to-myofibroblast (FibroMF) differentiation is crucial for embryogenesis and organ fibrosis. Although transforming growth factor-β (TGFβ) is the primary mediator of FibroMF differentiation, the type-I receptor (TGFβRI) responsible for this has not yet been confirmed. In the current study, we investigated the ALK1 and ALK5 expressions in TGFβ1-stimulat...
Source: Canadian Journal of Physiology and Pharmacology - July 23, 2020 Category: Drugs & Pharmacology Authors: Gah A, Adil MS, Sabbineni H, Verma A, Somanath PR Tags: Can J Physiol Pharmacol Source Type: research

Different effect of amiodarone and dofetilide on the dispersion of repolarization between well coupled ventricular and Purkinje fibers.
In conclusion, amiodarone decreased the dispersion between PF and VM, while dofetilide increased it. The measured difference in APD between cardiac regions may be the affected by electrotonic coupling, thus studying PF and VM separately may lead to an over or underestimation of dispersion. PMID: 32692935 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 21, 2020 Category: Drugs & Pharmacology Authors: Árpádffy-Lovas T, Husti Z, Baczkó I, Varró A, Virág L Tags: Can J Physiol Pharmacol Source Type: research

Telomere shortening velocity of patients administered with hypnotics is accelerated in a gender-differential manner.
Abstract The telomere length and its distribution were compared between patients administered with and without hypnotics to see if regular administration of hypnotics is associated with their aging-related somatic telomere shortening. Male patients presented significant shortening of telomere of circulating leukocytes in association with age (-41.9bp/year, p=0.045) in contrast to controls (-18.3kb/year, p=0.155). On the other hand, female patients presented no significant shortening of telomere length with aging (-16.4bp/year, p=0.372) in contrast to controls (-55.9bp/year, p=0.00005). These results suggested that...
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Maeda T, Horiuchi T, Makino N Tags: Can J Physiol Pharmacol Source Type: research

Exercise training suppresses Mst1 activation and attenuates myocardial dysfunction in mice with type 1 diabetes.
In conclusion, chronic exercise training attenuated myocardial dysfunction in mice with type 1 diabetes, at least in part, through suppressing Mst1 activation. PMID: 32687725 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Zhao HX, Zhang Z, Zhou HL, Hu F, Yu Y Tags: Can J Physiol Pharmacol Source Type: research

Knockdown of Bone Morphogenetic Protein Type II Receptor Leads to Decreased Aquaporin 1 Expression and Function in Human Pulmonary Microvascular Endothelial Cells.
In conclusion, decreased BMPR2 appears to affect Aqp1 at mRNA, protein, and functional levels. This observation may identify a contributory mechanism for PAH. PMID: 32687728 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Vassiliou AG, Keskinidou C, Kotanidou A, Frantzeskaki F, Dimopoulou I, Langleben D, Orfanos SE Tags: Can J Physiol Pharmacol Source Type: research

Leptin induces a contracting effect on guinea pig tracheal smooth muscle via the Ob-R receptor mechanism: novel evidence.
Abstract AIM: To explore the potential contracting effect of leptin on isolated guinea pig tracheal smooth muscle (TSM), the possible mechanism, and the impact of epithelium denudation or allergen sensitization. METHODS: An in vitro experiment investigated the effect of leptin at the concentration (250 - 100 nM) on isolated guinea pig TSM with an intact or denuded epithelium. Ovalbumin (OVA) and IgE were used to test the impact of active and passive sensitization. The isolated TSM strips were incubated in Krebs solution and aerated with carbogen (95% O2 and 5% CO2) via an automated tissue organ bath system (n...
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Magzoub AA, Al-Ayed M, Shaikh IA, Habeeb MS, Alshaibari KS, Shalayel MHF Tags: Can J Physiol Pharmacol Source Type: research

Role of mitochondria in the differential action of sodium deoxycholate and ursodeoxycholic acid on rat duodenum.
In conclusion, the inhibitory effect of NaDOC on intestinal Ca2+ absorption is mediated by mitochondrial dysfunction, which is avoided by UDCA. The stimulatory effect of UDCA alone is associated with amelioration of mitochondrial functioning. This knowledge could improve treatment of diseases that affect the intestinal Ca2+ absorption. PMID: 32687730 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Pérez A, Rivoira M, Rodriguez V, Marchionatti A, Tolosa de Talamoni N Tags: Can J Physiol Pharmacol Source Type: research

Sex-dependent effect of perinatal hypoxia on cardiac tolerance to oxygen deprivation in adults.
Abstract Epidemiological studies have demonstrated relationship between adverse influence of perinatal development and increased risk of ischemic heart disease in adults. From negative factors to which the fetus is subjected, the most important is hypoxia. The fetus may experience hypoxic stress under different conditions, including pregnancy at high altitude, pregnancy with anemia, placental insufficiency, and heart, lung and kidney disease. One of the most common insults during early stages of postnatal development is hypoxemia due to congenital cyanotic heart defects. Experimental studies have demonstrated a li...
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Ostadal B, Ostadalova I, Szarszoi O, Netuka I, Olejnickova V, Hlavackova M Tags: Can J Physiol Pharmacol Source Type: research

Dual mechanism of modulation of NaV1.8 sodium channels by ouabain.
Abstract In the primary sensory neuron, ouabain activates the dual mechanism that modulates the functional activity of NaV1.8 channels. Ouabain at endogenous concentrations (EO) triggers two different signaling cascades, in which the Na,K-ATPase/Src complex is the EO target and the signal transducer. The fast EO effect is based on modulation of the NaV1.8 channel activation gating device. EO triggers the tangential signaling cascade along the neuron membrane from Na,K-ATPase to the NaV1.8 channel. It evokes a decrease in effective charge transfer of the NaV1.8 channel activation gating device. Intracellular applic...
Source: Canadian Journal of Physiology and Pharmacology - July 20, 2020 Category: Drugs & Pharmacology Authors: Plakhova VB, Penniyaynen VA, Rogachevskii IV, Podzorova SA, Khalisov MM, Ankudinov AV, Krylov BV Tags: Can J Physiol Pharmacol Source Type: research

The Possible Protective Effect of Colchicine against Liver Damage Induced by Renal Ischemia-Reperfusion Injury: Role of Nrf2 and NLRP3 inflammasome.
CONCLUSION: Colchicine attenuates renal IRI-induced liver injury in rats. This effect may be due to reducing inflammation and oxidative stress markers. PMID: 32640174 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 8, 2020 Category: Drugs & Pharmacology Authors: Awad AS, Sallam AS, Elariny HAF Tags: Can J Physiol Pharmacol Source Type: research

Current Therapies Under Investigation for COVID-19.
Abstract In response to the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), researchers are expeditiously searching for antiviral treatments able to alleviate the symptoms of infection, which can be life-threatening. Here, we provide a general overview of what is currently known about the structure and characteristic features of SARS-CoV-2, some of which could potentially be exploited for the purposes of antiviral therapy and vaccine development. This mini-review also covers selected and noteworthy antiviral agents/supportive therapy out of hundreds of drugs that are being repurposed or t...
Source: Canadian Journal of Physiology and Pharmacology - July 8, 2020 Category: Drugs & Pharmacology Authors: Weisberg E, Sattler M, Yang PL, Parent A, Gray N, Griffin JD Tags: Can J Physiol Pharmacol Source Type: research

The influences of chokeberry extract supplementation on redox status and body composition in handball players during competition phase.
Abstract The aim of our study was to investigate the influence of twelve-week consumption of chokeberry extract on redox status, body composition, lipid profile and biochemical parameters in active handball players. The study included 16 handball players, aged 16-24 years (20.26±2.86 years). Every morning before training players received 30mL of liquid chokeberry extract for 12 weeks, during regular competition season. The research consisted of morphofunctional and biochemical testing, which was performed at three points (at the beginning of the study, 6 and 12 weeks after extract consumption). After the ch...
Source: Canadian Journal of Physiology and Pharmacology - July 8, 2020 Category: Drugs & Pharmacology Authors: Cikiriz N, Milosavljevic I, Jakovljevic B, Bolevich S, Jeremic J, Nikolic Turnic TR, Mitrovic M, Srejovic IM, Bolevich S, Jakovljevic V Tags: Can J Physiol Pharmacol Source Type: research

In vivo protective effects of UCMA against cartilage degeneration in monosodium iodoacetate-induced osteoarthritis model.
In conclusion, UCMA exhibited a therapeutic potential in the treatment of OA. This protective effect of UCMA is possibly achieved by reducing the aggrecanase activity and the production of inflammatory cytokines. PMID: 32640182 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 8, 2020 Category: Drugs & Pharmacology Authors: Okuyan HM, Terzi MY, Karaboga İ, DoĞan S, Kalaci A Tags: Can J Physiol Pharmacol Source Type: research

Endothelin-1 Induces Lysyl Oxidase Expression in Pulmonary Artery Smooth Muscle Cells.
CONCLUSIONS: Overexpressed endothelin-1 in PAH patients can increase expression of LOx and agitate cross-linking of elastin and collagen resulting in ectopic deposition of them in the vascular media. PMID: 32615041 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - July 2, 2020 Category: Drugs & Pharmacology Authors: Maruyama H, Sakai S, Dewachter L, Dewachter C, Rondelet B, Naeije R, Ieda M Tags: Can J Physiol Pharmacol Source Type: research

Evaluation of antioxidant activity and toxicity of sulfur- or selenium-containing 4-(arylchalcogenyl)-1H-pyrazoles.
In conclusion, the results demonstrated the antioxidant action of pyrazol derivative compounds in in vitro assays. Furthermore, the results showed low toxicity of compounds in in vivo assays. PMID: 32597688 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 29, 2020 Category: Drugs & Pharmacology Authors: Hartwig de Oliveira D, Sousa FSS, Birmann PT, Pesarico AP, Alves D, Jacob RG, Savegnago L Tags: Can J Physiol Pharmacol Source Type: research

Sovateltide (IRL-1620) affects neuronal progenitors and prevents cerebral tissue damage after ischemic stroke.
Abstract Stimulation of endothelin-B receptors by its agonist, IRL-1620 (INN: sovateltide), provides neuroprotection and neurological and motor function improvement following cerebral ischemia. We investigated the effect of sovateltide on stem and progenitor cells mediated neural regeneration and its effect on the cerebral tissue repair, and restoration of neurological and motor function. Sovateltide (5 µg/kg) was injected intravenously in permanent middle cerebral artery occluded (MCAO) rats at 4, 6, and 8 hrs at day 0, 3 and 6. Neurological and motor function tests were carried out pre-MCAO and at day 7 po...
Source: Canadian Journal of Physiology and Pharmacology - June 23, 2020 Category: Drugs & Pharmacology Authors: Ranjan AK, Briyal S, Khandekar D, Gulati A Tags: Can J Physiol Pharmacol Source Type: research

Changes in c-fos and p-CREB signaling following exposure to forced swim stress or exogenous corticosterone during morphine-induced place preference are dependent on glucocorticoid receptor in the basolateral amygdala.
In conclusion, it seems that the intra-BLA administration of RU38486 differently modulates the effect of morphine-induced CPP on the expression of c-fos and p-CREB/CREB ratio in animals that underwent FSS or corticosterone administration. PMID: 32574519 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 23, 2020 Category: Drugs & Pharmacology Authors: Razavi Y, Karimi S, Karimi-Haghighi S, Hesam S, Haghparast A Tags: Can J Physiol Pharmacol Source Type: research

Mechanism and effects of fructose diphosphate on anti-hypoxia fatigue and learning memory ability.
This study aims to investigate the mechanisms through which Fructose Diphosphate (FDP) causes the anti-hypoxia and anti-fatigue effects, and improves learning and memory. Mice were divided into three groups: FDP-L,FDP-H and a control group. Acute toxic hypoxia induced by carbon monoxide, sodium nitrite and potassium cyanide, and acute cerebral ischemic hypoxia, were used to investigate the anti-hypoxia ability of FDP. The tests of rod-rotating, mouse-tail suspension and swimming endurance were used to explore the anti-fatigue effects of FDP. The Morris water maze experiment was used to determine the impact of FDP on lear...
Source: Canadian Journal of Physiology and Pharmacology - June 18, 2020 Category: Drugs & Pharmacology Authors: Liu C, Shao C, Du Q, He C, Sun X, Lou A, Ma Z, Yu J Tags: Can J Physiol Pharmacol Source Type: research

Analgesia Additive Interaction between Tadalafil and Morphine in Experimental Animal Model.
Abstract Since both morphine and tadalafil have been proven to exert some of their analgesic activity through modulation of the NO-cGMP pathway, the aim of the current study is to evaluate the pharmacologic interaction between tadalafil and morphine in order to decrease the dose of morphine and subsequently its side effects. The assessment was carried out through isobolographic analysis relative to ED50s of both morphine and tadalafil obtained by tail-flick test on BALB/c mice. Morphine and tadalafil ED50s calculated from the dose-response curves were 8303 µg/kg and 2080 µ/kg, respectively. The experim...
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Mehanna MM, Domiati S, Nakkash Chmaisse H, El Mallah A Tags: Can J Physiol Pharmacol Source Type: research

Galectin-3 inhibition attenuates doxorubicin-induced cardiac dysfunction by upregulating the expression of peroxiredoxin-4.
In conclusion, Gal-3 mediated Dox-induced cardiotoxicity and Gal-3 inhibition attenuated Dox-induced cardiac dysfunction by upregulating the expression of Prx-4 to reduce myocardial oxidative stress. PMID: 32516552 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Tian Y, Lv W, Lu C, Jiang Y, Yang X, Song M Tags: Can J Physiol Pharmacol Source Type: research

Identification of differential expressed miRNA 48 hours after cerebral ischemia reperfusion injury in mice by the technique of miRNA microarray.
CONCLUSION: A number of miRNAs were significantly regulated in CIRI model which suggested miRNA was closely associated with the pathological alterations after ischemia. These identified miRNAs may provide directions and targets for the future pathological research of CIRI. PMID: 32516555 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Zhang Y, Ding N, Yi H, Zhao Y, Ye Z, Shen L, Li Z, Zhu Y Tags: Can J Physiol Pharmacol Source Type: research

Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro.
Abstract Levetiracetam(LEV) has been demonstrated to improve cognitive function. Hippocampal theta rhythm (4-12 Hz) is associated with a variety of cognitively related behaviors, such as exploration in both humans and animal models. We investigated the effects of LEV on the theta rhythm in the rat hippocampal CA3 in hippocampal slices in vitro. We found that LEV increased the theta power in a dose-dependent manner. The increase in theta power can be blocked by GABAA receptor(GABAAR) or NMDA receptor(NMDAR) antagonists but not by AMPA receptor antagonist, indicating the involvement of GABAAR and NMDAR in the induct...
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Xing H, Xu S, Xie X, Wang Y, Lu C, Han X Tags: Can J Physiol Pharmacol Source Type: research

Mode of delivery alters dental pulp nociception and pain-induced changes in cognitive performance in adults' male rats.
This study examined the effects of delivery mode on the response to inflammatory pulpal pain and pain-induced changes in cognitive performance in adult rats. Experiments were done on rats born by vaginal or caesarean section (C-section) delivery. Dental pulp was irritated by intradental capsaicin (100 µg) application and then nociceptive scores were recorded for 40 min. Spatial and passive avoidance learning and memory were assessed using Morris Water Maze (MWM) and shuttle box tools, respectively. Additionally, in vivo recording of field excitatory postsynaptic potential (fEPSP) in the CA1 of the hippocampus, was us...
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Mohamadi-Jorjafki E, Abbasnejad M, Kooshki R, Esmaeili-Mahani S, Raoof M Tags: Can J Physiol Pharmacol Source Type: research

Diabetes-related sex differences in the brain endothelin system following ischemia in vivo and in human brain endothelial cells in vitro.
Abstract The endothelin (ET) system has been implicated to contribute to the pathophysiology of cognitive impairment and stroke in experimental diabetes. Our goals were to test the hypotheses that 1) circulating and/or peri-infarct ET-1 levels are elevated after stroke in both sexes and this increase is greater in diabetes, 2) ET receptors are differentially regulated in the diabetic brain, 3) brain microvascular endothelial cells (BMVEC) of female and male origin express the ETA receptor subtype, and 4) diabetes and stroke-mimicking conditions increase ET-1 levels in BMVECs of both sexes. Control and diabetic rat...
Source: Canadian Journal of Physiology and Pharmacology - June 4, 2020 Category: Drugs & Pharmacology Authors: Abdul Y, Li W, Vargas JD, Grant E, He L, Jamil S, Ergul A Tags: Can J Physiol Pharmacol Source Type: research

Gestational postprandial insulin sensitivity in the Sprague Dawley rat: the putative role of Hepatic Insulin Sensitizing Substance (HISS) in glucose partitioning in pregnancy.
Abstract Pregnancy requires adaptation of maternal insulin sensitivity. In the fed state, a pulse of insulin stimulates glucose uptake and nutrient energy storage via insulin-dependent as well as Hepatic Insulin Sensitizing Substance (HISS)-dependent action. HISS is released by the liver in the fed state in the presence of signals integrated through the liver and a pulse of insulin. HISS promotes glucose storage as glycogen in heart, kidney and skeletal muscle but not gut, liver or adipose tissue. HISS is also responsible for the vasodilatory action previously attributed to insulin. The Rapid Insulin Sensitivity T...
Source: Canadian Journal of Physiology and Pharmacology - May 26, 2020 Category: Drugs & Pharmacology Authors: Lovat NEJ, Legare DJ, Gieni RS, Lautt WW Tags: Can J Physiol Pharmacol Source Type: research

History of the Pharmacological Society of Canada 1956-2008.
Abstract The Pharmacological Society of Canada (PSC) formed in 1956 and became a constituent society of the Canadian Federation of Biological Sciences (CFBS) in 1958. Over subsequent decades, it met annually with CFBS, matured as a society, and established an identity as the voice of pharmacology in Canada. During the 1980s, it sought a larger stage and bid for, and then hosted, the XIIth International Congress of Pharmacology in Montreal in 1994. The society then participated in several joint meetings with other national pharmacology societies. In 2008, the PSC merged with the Canadian Society for Clinical Pharma...
Source: Canadian Journal of Physiology and Pharmacology - May 25, 2020 Category: Drugs & Pharmacology Authors: Sawynok J, Nakatsu K Tags: Can J Physiol Pharmacol Source Type: research

Knockout of the Circadian Clock Protein PER1 Results in Sex-Dependent Alterations of ET-1 Production in Mice in Response to a High Salt Diet plus Mineralocorticoid Treatment.
Abstract Previously, we showed that global knockout (KO) of the circadian clock transcription factor PER1 in male, but not female, mice fed a high salt diet plus mineralocorticoid treatment (HS/DOCP) resulted in non-dipping hypertension and decreased night/day ratio of sodium (Na) excretion. Additionally, we have shown that the endothelin-1 (ET-1) gene is targeted by both PER1 and aldosterone. We hypothesized that ET-1 would exhibit a sex-specific response to HS/DOCP treatment in PER1 KO. Here we show that male, but not female, global PER1 KO mice exhibit a decreased night/day ratio of urinary ET-1. Gene expressio...
Source: Canadian Journal of Physiology and Pharmacology - May 21, 2020 Category: Drugs & Pharmacology Authors: Douma LG, Crislip GR, Cheng KY, Barral D, Masten S, Holzworth MR, Roig E, Glasford K, Beguiristain K, Li W, Bratanatawira P, Lynch IJ, Cain BD, Wingo CS, Gumz ML Tags: Can J Physiol Pharmacol Source Type: research

Predictive model of bosentan-induced liver toxicity in Japanese patients with pulmonary arterial hypertension.
Abstract Bosentan, an endothelin receptor antagonist, has been widely used as a first-line medication for the treatment of pulmonary arterial hypertension (PAH). However, liver dysfunction is a major side effect of bosentan treatment that could hamper the optimal management of patients with PAH. Previously, we demonstrated, using drug metabolism enzymes and transporters (DMET) analysis, that the carbohydrate sulfotransferase 3 (CHST3) and CHST13 alleles are significantly more frequent in patients with elevated amino-transferases during therapy with bosentan than they are in patients without liver toxicity. In addi...
Source: Canadian Journal of Physiology and Pharmacology - May 20, 2020 Category: Drugs & Pharmacology Authors: Yorifuji K, Uemura Y, Horibata S, Tsuji G, Suzuki Y, Nakayama K, Hatae T, Kumagai S, Emoto N Tags: Can J Physiol Pharmacol Source Type: research

Correction: A comparative, single-blind, randomized study on quetiapine and aripiperazole augmentation in treatment of selective serotonin reuptake inhibitor refractory obsessive-compulsive disorder.
PMID: 32383985 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - May 8, 2020 Category: Drugs & Pharmacology Authors: Talaei A, Hosseini FF, Aghili Z, Akhondzadeh S, Asadpour E, Mehramiz NJ, Forouzanfar F Tags: Can J Physiol Pharmacol Source Type: research

Correction: Intracrine signaling through lipid mediators and their cognate nuclear G-protein-coupled receptors: a paradigm based on PGE2, PAF, and LPA1 receptors.
PMID: 32379492 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - May 7, 2020 Category: Drugs & Pharmacology Authors: Zhu T, Gobeil F, Vazquez-Tello A, Leduc M, Rihakova L, Bossolasco M, Bkaily G, Peri K, Varma DR, Orvoine R, Chemtob S Tags: Can J Physiol Pharmacol Source Type: research

Endokinin A/B stimulates rat gastric motility through myogenic NK1 receptors located in fundus.
Abstract Endokinin A/B (EKA/B), the common C-terminal decapeptide in endokinins A and B, is a preferred ligand of NK1 receptor and regulates pain and itch. The study focused on the effects of EKA/B on rat gastric motility in vivo and in vitro. Gastric emptying was measured to evaluate gastric motility in vivo. Intragastric pressure and the contraction of gastric muscle strips were measured to evaluate gastric motility in vitro. Moreover, various neural blocking agents and neurokinin receptor antagonists were applied to explore the mechanisms. TAC4 and TACR1 mRNA were expressed throughout rat stomach. EKA/B promote...
Source: Canadian Journal of Physiology and Pharmacology - May 4, 2020 Category: Drugs & Pharmacology Authors: Zhang J, Ma C, Wang R, He C, Li H, Dong S Tags: Can J Physiol Pharmacol Source Type: research

Folic acid affects cardiometabolic, oxidative stress and immunohistochemical parameters in monocrotaline-induced rat heart failure.
In this study, we explored the effects of folic acid (FA) on cardiometabolic, oxidative stress-biomarker changes and the activity of proliferation marker Ki67 in monocrotaline-induced HF. The research was conducted during a 4-week period, using 5 experimental groups (8 animals/group): blank solution-exposed controls (C1 physiological saline 1ml/kg one day; C2 physiological saline 1ml/kg 28 days), MCT-induced HF (MCT 50mg/kg), FA (FA 5mg•kg-1•day-1) and MCT+FA (MCT 50mg/kg, FA 5mg•kg-1•day-1). Superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities together with total glutathione and param...
Source: Canadian Journal of Physiology and Pharmacology - April 30, 2020 Category: Drugs & Pharmacology Authors: Jakovljevic Uzelac J, Djukic T, Radic T, Mutavdzin S, Stankovic S, Rakocevic J, Labudovic Borovic M, Milic N, Simic T, Savic Radojevic A, Djuric D Tags: Can J Physiol Pharmacol Source Type: research

Endothelin-1, endothelin receptor antagonists and vein graft occlusion in coronary artery bypass surgery: twenty years on and still no journey from bench to bedside.
Abstract The saphenous vein is the most commonly used bypass graft in patients with coronary artery disease. During routine coronary artery bypass grafting the vascular damage inflicted on the vein is likely to stimulate the release of endothelin-1, a potent endothelium-derived vasoconstrictor that also possesses cell proliferation and inflammatory properties, conditions associated with vein graft failure. In both in vitro and in vivo studies, endothelin receptor antagonists reduce neointimal thickening. The mechanisms underlying these observations are multi-factorial and include an effect on cell proliferation an...
Source: Canadian Journal of Physiology and Pharmacology - April 28, 2020 Category: Drugs & Pharmacology Authors: Dashwood M, Loesch A Tags: Can J Physiol Pharmacol Source Type: research

Chondroitin sulfate mediates liver responses to injury induced by dual endothelin receptor inhibition.
In this study we investigated whether dual ER inhibition in the liver could alter CHST3 and CHST13 expression and thus CS production, and whether liver CS content could prevent inflammatory and fibrosis responses after liver injury. We observed increased CHST3 and CHST13 expression after liver injury in bile duct-ligated mice and histologically confirmed abundant CS deposition in the injured liver. Moreover, treating Hep3B cells with a dual ERA mimic significantly increased CHST3 and CHST13 expression, inflammatory cytokine levels, and glycosaminoglycan deposition. Furthermore, pro-inflammatory and pro-fibrotic markers wer...
Source: Canadian Journal of Physiology and Pharmacology - April 21, 2020 Category: Drugs & Pharmacology Authors: Ryanto GRT, Yorifuji K, Ikeda K, Emoto N Tags: Can J Physiol Pharmacol Source Type: research

The ratio of circulating endothelin-1:endothelin-3 associated with TIMI risk and dynamic TIMI risk score in ST elevation acute myocardial infarction.
In conclusion, combining the ET-1 and ET-3 levels into the ET-1:ET-3 ratio provided a prognostic value by independently predicting the increased risk to 1-year mortality as indicated by at discharge dynamic TIMI risk score in patients with STEMI. PMID: 32315546 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - April 21, 2020 Category: Drugs & Pharmacology Authors: Hartopo AB, Puspitawati I, Mumpuni H Tags: Can J Physiol Pharmacol Source Type: research

Galectin-3 mediates high-glucose-induced cardiomyocyte injury by NADPH oxidase/reactive oxygen species pathway.
This study was designed to study the improved effect of galectin-3 inhibition on diabetic cardiomyopathy (DCM). Sprague-Dawley rats were randomized into the control, DCM, and DCM + modified citrus pectin (MCP, a galectin-3 pharmacological inhibitor) groups. After 8 weeks, streptozotocin (STZ)-induced DCM led to high blood glucose level, oxidative stress, cardiac injury, and dysfunction, accompanied by suppressed body weight. On the contrary, MCP (100 mg/kg/day) administration improved body weight and blood glucose level and attenuated cardiac injury and dysfunction in DCM rats. Additionally, MCP attenuated pathological cha...
Source: Canadian Journal of Physiology and Pharmacology - April 20, 2020 Category: Drugs & Pharmacology Authors: Sun J, Zhang L, Fang J, Yang S, Chen L Tags: Can J Physiol Pharmacol Source Type: research

C-type natriuretic peptide (CNP)/Guanylate cyclase B (GC-B) system and endothelin-1(ET-1)/ET receptor A and B system in human vasculature.
Abstract In order to assess the physiological and clinical implications of C-type natriuretic peptide (CNP)/guanylyl cyclase B (GC-B) system in the human vasculature, we have examined gene expressions of CNP and its receptor, GC-B, in human vascular endothelial cells (ECs) and smooth muscle cells (SMCs) and have also compared endothelin-1(ET-1)/endothelin receptor-A (ETR-A) and endothelin receptor-B (ETR-B) system in human aortic ECs (HAECs) and vascular SMCs (HSMCs) in vitro. We also examined these gene expressions in human embryonic stem (ES)/induced pluripotent stem cell (iPS)-derived ECs and mural cells (MCs)....
Source: Canadian Journal of Physiology and Pharmacology - April 8, 2020 Category: Drugs & Pharmacology Authors: Taura D, Nakao K, Nakagawa Y, Kinoshita H, Sone M, Nakao K Tags: Can J Physiol Pharmacol Source Type: research

Circulating endothelial cell-derived microvesicles are elevated with hypertension and associated with endothelial dysfunction.
CIRCULATING ENDOTHELIAL CELL-DERIVED MICROVESICLES ARE ELEVATED WITH HYPERTENSION AND ASSOCIATED WITH ENDOTHELIAL DYSFUNCTION. Can J Physiol Pharmacol. 2020 Apr 08;: Authors: Stockelman KA, Hijmans JG, Bammert T, Greiner JJ, Stauffer BL, DeSouza C Abstract The purpose of this study was to determine: 1) if circulating endothelial microvesicles (EMVs) are elevated in hypertensive adults; and 2) whether circulating EMVs are associated with hypertension-related endothelial vasodilator dysfunction. Circulating EMVs (CD31+/42b-) was determined in 30 middle-aged adults (55+1 years): 15 normotensive (10M/5F; ...
Source: Canadian Journal of Physiology and Pharmacology - April 8, 2020 Category: Drugs & Pharmacology Authors: Stockelman KA, Hijmans JG, Bammert T, Greiner JJ, Stauffer BL, DeSouza C Tags: Can J Physiol Pharmacol Source Type: research

MiR-142 and miR-212 synergistically inhibits the proliferation and collagen formation of TGF- β1-induced cardiac fibroblasts by regulating c-Myc/TP53INP1.
CONCLUSION: Overexpression of miR-142-5p/212-5p cooperatively suppress the proliferation and collagen formation after MI by regulating c-Myc/TP53INP1. PMID: 32268073 [PubMed - as supplied by publisher] (Source: Canadian Journal of Physiology and Pharmacology)
Source: Canadian Journal of Physiology and Pharmacology - April 8, 2020 Category: Drugs & Pharmacology Authors: Wang Z, Fu M, Li Y Tags: Can J Physiol Pharmacol Source Type: research