Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells.

Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells. J Inorg Biochem. 2019 Nov 23;203:110952 Authors: Giesen B, Nickel AC, Garzón Manjón A, Vargas Toscano A, Scheu C, Kahlert UD, Janiak C Abstract Glioblastoma (GBM) is an aggressive disease with currently no satisfying treatment option available. GBM cells with stem cell properties are thought to be responsible for the initiation and propagation of the disease, as well as main contributors to the emergence of therapy resistance. In this work, we developed a novel method to synthesize fluorescent gold nanoparticles as potential drug and gene delivery systems for GBM therapy, able to penetrate three-dimensional stem cell selected patient-derived GBM neurosphere systems in vitro. By using polyethylene imine (PEI) as a stabilizer and reducing agent, as well as fluorescein isothiocyanate (FITC) as a fluorescent marker, our fully in-house developed fluorescent gold nanoparticles (AuPEI-FITC NPs) with core sizes between 3 and 6 nm were obtained via a fast microwave-assisted reaction. Cytotoxicity, adsorption and internalization of AuPEI-FITC NPs into the cell lines JHH520, 407 and GBM1 were investigated using the cellular growth assay and fluorescence-activated cell sorting (FACS) analysis. AuPEI-FITC NPs showed no apparent cytotoxicity and an uptake in cells of up to ~80%. A differentiation between surface-bound and intern...
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research

Related Links:

Conclusions: Treatment outcome of PRCA depended upon the causes and the types of treatment, and the burden of RBC transfusion was very high in patients with ESA and thymoma-associated PRCA. PMID: 32257434 [PubMed]
Source: Anemia - Category: Hematology Tags: Anemia Source Type: research
[Stem cells in regenerative medicine - from bench to bedside]. Handchir Mikrochir Plast Chir. 2020 Apr 07;: Authors: Klietz ML, Kückelhaus M, Kaiser HW, Raschke MJ, Hirsch T, Aitzetmüller M Abstract The use of mesenchymal stem cells for regenerative medicine is becoming more popular than ever before - even though their clinical usage is limited. Many ethical and translational questions, as well as legal aspects and uncertainty regarding safety inhibit the growth of appropriate therapies from promising scientific approaches.This paper demonstrates the main problems of the t...
Source: Handchirurgie Mikrochirurgie Plastische Chirurgie - Category: Cosmetic Surgery Tags: Handchir Mikrochir Plast Chir Source Type: research
CONCLUSION: The DAM Science Academy can be considered a highly suitable complemental platform to the existing networking opportunities among microsurgical researchers. Experience so far suggests that this will hopefully result in long-term cooperations and a permanent transfer of knowledge among the participants. PMID: 32259860 [PubMed - in process]
Source: Handchirurgie Mikrochirurgie Plastische Chirurgie - Category: Cosmetic Surgery Tags: Handchir Mikrochir Plast Chir Source Type: research
Abstract Maitake (Grifola frondosa) mushroom has received an enormous amount of attention as a dietary supplement due to its high nutritional values. The particle sizes of G. frondosa mushrooms were monitored by a classifying mill. β-Glucans are the bioactive component of the mushroom, and it was revealed through Fourier transform infrared spectroscopy (FTIR), proton and carbon nuclear magnetic resonance (1H and 13C-NMR), matrix-assisted laser desorption/ionization, and time-of-flight (MALDI-TOF) spectrometry. The biocompatibility of G. frondosa particles, as well as induced osteogenesis of hMSCs, was evaluat...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Abstract The development of colorectal cancer (CRC) is a multistep process initiated by a benign polyp that has the potential to evolve into in situ carcinoma through the interactions between environmental and genetic factors. CRC incidence rates are constantly increased for young adult patients presenting an advanced tumor stage. The majority of CRCs arise from colonic adenomas originating from aberrant cell proliferation of colon epithelium. Endoscopic polypectomy represents a tool for early detection and removal of polyps, although the occurrence of cancers after negative colonoscopy shows a significant inciden...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Publication date: Available online 8 April 2020Source: Stem Cell ResearchAuthor(s): Viviana Meraviglia, Christiaan H. Arendzen, Christian Freund, Douwe E. Atsma, Christine L. Mummery, Milena Bellin
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 7 April 2020Source: Stem Cell ResearchAuthor(s): Chuanbo Sun, Pei Zhou, Simin Yuan, Ruirui Guo, Zhan Zhang, Qingyang Xu, Dingding Han, Lu He, Hongmei Tang, Kaishou Xu, Hao Hu, Na Li
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: 7 April 2020Source: Cell Reports, Volume 31, Issue 1Author(s): Hiraku Tsujimoto, Tomoko Kasahara, Shin-ichi Sueta, Toshikazu Araoka, Satoko Sakamoto, Chihiro Okada, Shin-ichi Mae, Taiki Nakajima, Natsumi Okamoto, Daisuke Taura, Makoto Nasu, Tatsuya Shimizu, Makoto Ryosaka, Zhongwei Li, Masakatsu Sone, Makoto Ikeya, Akira Watanabe, Kenji Osafune
Source: Cell Reports - Category: Cytology Source Type: research
Conclusion: Planned irradiation of potential stem cell niches in the ipsilateral cerebral hemisphere did not result in improved survival as suggested by retrospective studies. Doses to contralateral or bilateral PVZ or SVZ also did not influence survival. PMID: 32256704 [PubMed - as supplied by publisher]
Source: Ecancermedicalscience - Category: Cancer & Oncology Tags: Ecancermedicalscience Source Type: research
Authors: Stukenborg JB, Wyns C Abstract Genetic parenthood following cancer therapy is considered to be a major factor of quality of life. Given the rising proportion of patients surviving cancer due to improved therapeutic protocols, it is an issue of growing importance. Hence, the efforts to preserve fertility have motivated researchers to develop options for the paediatric population facing fertility-threatening cancer therapies. In prepubertal boys who do not yet produce sperm, cryo-banking of testicular tissue containing spermatogonial stem cells (SSCs) is the only viable option for future fertility preservati...
Source: Ecancermedicalscience - Category: Cancer & Oncology Tags: Ecancermedicalscience Source Type: research
More News: Biochemistry | Brain | Genetics | Nanotechnology | Neurology | Stem Cell Therapy | Stem Cells