Phylo-diversity of prevalent human E. coli O157:H7 with strains from retailed meat and fish in selected markets in Ibadan Nigeria.

Phylo-diversity of prevalent human E. coli O157:H7 with strains from retailed meat and fish in selected markets in Ibadan Nigeria. J Immunoassay Immunochem. 2019 Nov 24;:1-15 Authors: Ayodele OA, Deji-Agboola AM, Akinduti PA, Faneye AO Abstract Global prevalence of ESBL-biotypes poses a serious threat to public health as a result of severity and morbidity caused by beta-lactam encoded Escherichia coli. Therefore, the prevalent shiga toxigenic Escherichia coli of ESBL variant was investigated in various retailed food animals and cooking materials. A total of 823 samples consisting of raw meat (297) and fish (132) samples retailed at various major markets in Ibadan were collected and 394 swabs were taken from the butchers' processing tables and utensils used in retailing meat and fish. The samples were cultured and biotyped for Escherichia coli. Serological and PCR assay were used to identify O157:H7 variant and antibiotics resistant determinants. Genetic relatedness of characterized E. coli O157 from human and meat products was evaluated with phylogenetic analysis. Of all the isolates, 130 (15.8%) were E. coli and only 8 (1.0) were O157:H7 while 4 (50%) were resistant to one or more antibiotics with resistance index ranging from 0.1 to 0.5. More than 25% E. coli O157:H7 were resistant to Ampicillin, Pefloxacin and Gentamicin and blaSHV and blaCTX-M were detected in 1/8 (12.5%) of E. coli O157:H7 and blaTEM 3/8 (37.5%) respectively. Only 1 genotyped human E...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

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Publication date: Available online 20 March 2020Source: Fish &Shellfish ImmunologyAuthor(s): Tingting Li, Quanwei Liu, Haitao Chen, Jianrong Li
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Source: Biochimica et Biophysica Acta (BBA) General Subjects - Category: Biochemistry Source Type: research
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Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
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Source: American Journal of Clinical Oncology - Category: Cancer & Oncology Tags: Original Articles: Gastrointestinal Source Type: research
In this study, we analyzed the epidemiological and genetic features of NoV outbreaks from July 2014 to June 2018 in Huzhou, China. A total of 450 stool samples collected from 51 AGE outbreaks were tested for NoVs by real-time RT PCR. Partial polymerase and capsid sequences of NoV-positive samples were amplified and sequenced for phylogenetic analysis. NoVs were found to be responsible of 84.3% of AGE outbreaks in Huzhou over the past 5 years. Most NoV outbreaks were reported in the cool months (November-March) and occurred in primary schools and kindergartens. Changes in the diversity of genotypes and the distribution of p...
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Journal of Animal Physiology and Animal Nutrition - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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