Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016.

Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016. Korean J Parasitol. 2019 Oct;57(5):531-536 Authors: Ma DW, Lee MR, Hong SH, Cho SH, Lee SE Abstract Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013-2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for conti...
Source: Korean Journal of Parasitology - Category: Parasitology Tags: Korean J Parasitol Source Type: research

Related Links:

The coronavirus outbreak started in central China and has left 41 people dead.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
In this study, we report the epidemiological, clinical, laboratory, radiological, and microbiological findings of five patients in a family cluster who presented with unexplained pneumonia after returning to Shenzhen, Guangdong province, China, after a visit to Wuhan, and an additional family member who did not travel to Wuhan. Phylogenetic analysis of genetic sequences from these patients were done.FindingsFrom Jan 10, 2020, we enrolled a family of six patients who travelled to Wuhan from Shenzhen between Dec 29, 2019 and Jan 4, 2020. Of six family members who travelled to Wuhan, five were identified as infected with the ...
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 24 January 2020Source: The LancetAuthor(s): David L Heymann
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 24 January 2020Source: The LancetAuthor(s): Chen Wang, Peter W Horby, Frederick G Hayden, George F Gao
Source: The Lancet - Category: General Medicine Source Type: research
President Xi Jinping said China was facing a "grave situation" as the death toll from the coronavirus outbreak jumped to 42, overshadowing celebrations of the Lunar New Year that began on Saturday.
Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
Chinese communities around the world greeted the Year of the Rat on Saturday with dances and parades, although celebrations were marred by worries about the coronavirus outbreak that has upended Lunar New Year festivities in China.
Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
This study suggests that intestinal inflammation and reduction of short chain fatty acids (SCFAs) caused by the imbalance of gut microbiota are crucial to the pathogenesis of T1DM.Graphical abstractIn this study, LEfSe multilevel species hierarchical tree directly reflects the differences of gut microbiota between control and T1DM rats at the Phylum-to-Family level, the result showed that the gut microbiome of T1DM rats changed significantly. The abundance of pathogenic bacteria associated with infection and inflammation in T1DM rats was up-regulated such as Ruminococcaceae, Shigella, Enterococcus, Streptococcus, Rothia an...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
Abstract Foodborne zoonotic pathogens are a serious public health issue and result in significant global economic losses. Despite their importance to public health, epidemiological data on foodborne diseases including giardiasis caused by the enteric parasite, Giardia duodenalis, are lacking. This parasite is estimated to cause ∼28.2 million cases of diarrhoea each year due to contamination of food, but very few foodborne outbreaks have been documented due to the limitations of current detection as well as surveillance methods. The current method for the recovery of Giardia cysts from food matrices using immun...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research
uml;rd SG Abstract Molecular epidemiology and genotyping studies of the parasitic protozoan Giardia intestinalis have proven difficult due to multiple factors, such as low discriminatory power in the commonly used genotyping loci, which has hampered molecular analyses of outbreak sources, zoonotic transmission and virulence types. Here we have focused on assemblage A Giardia and developed a high-resolution assemblage-specific multilocus sequence typing (MLST) method. Analyses of sequenced G. intestinalis assemblage A genomes from different sub-assemblages identified a set of six genetic loci with high genetic vari...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
More News: Children | Environmental Health | Epidemiology | Gastroenteritis | Genetics | Giardiasis | Outbreaks | Parasitic Diseases | Parasitology | Zoonoses