A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica.
In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects (P ≤ 0.001) than the simple drugs when the selectivity index was considered. The gene expression lev...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms.
In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms. PMID: 30840793 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat.
Authors: Kim JH, Lee K, Sohn WM, Kim HY, Lee YR, Choi EJ, So B, Jung JY Abstract A stray female cat of unknown age, presenting bright red watery diarrhea, was submitted to the Animal and Plant Quarantine Agency for diagnosis. In the small intestines extracted from the necropsied cat, numerous white oval-shaped organisms were firmly embedded in the mucosa and there was thickening of intestinal wall. Histopathological analysis revealed severe necrotizing enteritis, together with atrophied intestinal villi, exfoliated enterocytes, and parasitic worms. Recovered worms were identified as Pharyngostomum cordatum by morph...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men.
Authors: Kim JH, Moon HS, Kim KS, Hwang HS, Ryu JS, Park SY Abstract Trichomoniasis is the most common curable sexually-transmitted infection. Most Trichomonas vaginalis-infected men are asymptomatic and can remain undiagnosed and untreated, and this has been thought to result in chronic persistent prostatic infection. Chronic inflammation is regarded as the major factor in the pathogenesis and progression of benign prostatic hyperplasia (BPH) and prostatic cancer (PCa). The aim of this study is to identify seropositivity to T. vaginalis in men with prostate tumors (BPH or PCa) visited to Hanyang University Hospita...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Comparison of Two PCR Assays for Trichomonas vaginalis.
This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test. PMID: 30840796 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis.
Authors: Mendoza-Oliveros T, Arana-Argáez V, Alvaréz-Sánchez LC, Lara-Riegos J, Alvaréz-Sánchez ME, Torres-Romero JC Abstract Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTR...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Quantification of Immunoglobulin G against Trypanosoma cruzi in Individuals with Chronic Chagas Disease Treated with Nifurtimox and Evaluated in Prolonged Follow-Up.
This study aims to evaluate the efficacy of nifurtimox (NF) in the treatment of chronic ChD in prolonged follow-up by serological techniques of indirect immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA) IgG comparing 2 groups of patients, treated and non treated. Mann-Whitney test was performed for ELISA and IFA, with significant difference between the groups (P < 0.05). IgG levels were lower in individuals treated compared with untreated patients, indicating chemotherapeutic efficacy in prolonged follow-up. PMID: 30840798 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam.
Authors: Chien NTH, Nguyen TL, Bui KL, Nguyen TV, Le TH Abstract Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is abl...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro.
Authors: Boueroy P, Duenngai K, Eamudomkarn C, Sripan P, Boonmars T, Pumhirunroj B, Artchayasawat A, Songsri J, Chomphumee K, Rattanasuwan P, Laummaunwai P, Khueangchiangkhwang S, Boonjaraspinyo S Abstract Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of ...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Complete Sequence of the Mitochondrial Genome of Spirometra ranarum: Comparison with S. erinaceieuropaei and S. decipiens.
This study was undertaken to determine the complete mitochondrial DNA sequence and structure of the mitochondrial genome of Spirometra ranarum, and to compare it with those of S. erinaceieuropaei and S. decipiens. The aim of this study was to provide information of the species level taxonomy of Spirometra spp. using the mitochondrial genomes of 3 Spirometra tapeworms. The S. ranarum isolate originated from Myanmar. The mitochondrial genome sequence of S. ranarum was compared with that of S. erinaceieuropaei (GenBank no. KJ599680) and S. decipiens (GenBank no. KJ599679). The complete mtDNA sequence of S. ranarum comprised 1...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep.
Authors: Tianli L, Xifeng W, Zhenzhong T, Lixia W, Xingxing Z, Jun Q, Qingling M, Shasha G, Ying C, Xuepeng C Abstract Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Molecular Detection of Coxiella burnetii in Cattle on Ulleung Island, Korea: A Population-based Study with Four Years of Follow Up.
In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3-1.0% in the period 2011-2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide sequences of the 2 samples showed high (98.4-100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the transmission of C. burnetii on Ulleung Island, control strategy shou...
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Erratum: An Outbreak of Histomoniasis in Backyard Sanhuang Chickens.
Authors: PMID: 30840804 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Professor Seung-Yull Cho (1943-2019).
Authors: Kong Y Abstract PMID: 30840805 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - March 7, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Epidemiological Characteristics of Re-emerging Vivax Malaria in the Republic of Korea (1993-2017).
Authors: Bahk YY, Lee HW, Na BK, Kim J, Jin K, Hong YS, Kim TS Abstract Historically, Plasmodium vivax malaria has been one of the most highly endemic parasitic diseases in the Korean Peninsula. Until the 1970s, vivax malaria was rarely directly lethal and was controlled through the Korean Government Program administered by the National Malaria Eradication Service in association with the World Health Organization's Global Malaria Eradication Program. Vivax malaria has re-emerged in 1993 near the Demilitarized Zone between South and North Korea and has since become an endemic infectious disease that now poses a seri...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea.
Authors: Bahk YY, Kim J, Ahn SK, Na BK, Chai JY, Kim TS Abstract Plasmodium vivax is more challenging to control and eliminate than P. falciparum due to its more asymptomatic infections with low parasite densities making diagnosis more difficult, in addition to its unique biological characteristics. The potential re-introduction of incidence cases, either through borders or via human migrations, is another major hurdle to sustained control and elimination. The Republic of Korea has experienced re-emergence of vivax malaria in 1993 but is one of the 32 malaria-eliminating countries to-date. Despite achieving success...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii.
Authors: Moon EK, Choi HS, Park SM, Kong HH, Quan FS Abstract Pathogenic Acanthamoeba spp. cause granulomatous amoebic encephalitis and keratitis. Acanthamoeba keratitis (AK) is a rare but serious ocular infection that can result in permanent visual impairment or blindness. However, pathogenic factors of AK remain unclear and treatment for AK is arduous. Expression levels of proteins secreted into extracellular space were compared between A. castellanii pathogenic (ACP) and non-pathogenic strains. Two-dimensional polyacrylamide gel electrophoresis revealed 123 differentially expressed proteins, including 34 increas...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea.
This study is the first molecular detection of A. phagocytophilum in horses in Korea. Human granulocytic anaplasmosis and animal infection of A. phagocytophilum have been reported in Korea recently. Because of vector tick distribution, global warming, and the increase of the horse industry, horses should be considered as a potential reservoir for A. phagocytophilum, and cross infectivity should be evaluated even though a low prevalence of infection was detected in this study. Furthermore, continuous surveillance and effective control measures for A. phagocytophilum should be established to prevent disease distribution and ...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Morphological and Molecular Characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean Raccoon Dog, Nyctereutes procyonoides koreensis.
In conclusion, it was confirmed for the first time that T. tanuki is indigenously distributed, the Korean raccoon dog is acted as the natural definitive host of this nematode in Korea and the morphological characteristics of T. tanuki were shown in specific structure for single postcloacal median papilla in male. PMID: 30630277 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Impact of Single Dose Praziquantel Treatment on Schistosoma haematobium Infection among School Children in an Endemic Nigerian Community.
Authors: Adewale B, Mafe MA, Sulyman MA, Idowu ET, Ajayi MB, Akande DO, Mckerrow JH, Balogun EO Abstract Schistosomiasis is prevalent in Nigeria, and the foremost pathogen is Schistosoma haematobium, which affects about 29 million people. Single dose of the drug praziquantel is often recommended for treatment but the efficacy has not been documented in certain regions. Therefore, this study was designed to assess the impact of single dose praziquantel treatment on S. haematobium infection among school children in an endemic community of South-Western Nigeria. Urine samples were collected from 434 school children an...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences.
In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5' region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these spec...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Tick-Borne Pathogens in Ixodid Ticks from Poyang Lake Region, Southeastern China.
Authors: Zheng WQ, Xuan XN, Fu RL, Tao HY, Liu YQ, Liu XQ, Li DM, Ma HM, Chen HY Abstract Ticks are the vectors of various pathogens, threatening human health and animal production across the globe. Here, for the first time we detected Ricketssia spp., Borrelia spp. and protozoan in ticks from Poyang Lake region in Ji- angxi Province of eastern China. In 3 habitat categories and on 12 host species, 311 ticks from 11 species were collected. Haemaphysalis longicornis was the predominant species, accounting for 55.63%, followed by Rhipicephalus microplus, Haemaphysalis flava and Ixodes granulatus. Of the collected tic...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

An Outbreak of Histomoniasis in Backyard Sanhuang Chickens.
Authors: Liu D, Kong L, Tao J, Xu J Abstract Histomonas meleagridis is a facultative anaerobic parasite, which can cause a common poultry disease known as histomoniasis. The species and age of the birds impacts on the susceptibility, with turkey being the most susceptible species. Chickens are less susceptible to H. meleagridis than turkeys and usually serve as reservoir hosts. Here, the diagnosis of an outbreak of histomoniasis in backyard Sanhuang chickens is described. The primary diagnosis was made based on clinical symptoms, general changes at necropsy, histopathology, and the isolation and cultivation of para...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

The First Case of Diarrhoea in Tibetan Sheep, Ovis aries, Caused by Balantidium coli in the Qinghai Tibetan Plateau Area, China.
This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles. Macronucleus and contractile vacuoles could not be distinguished. Size of the trophozoite was 80-180 × 70-150 μm and its surface was covered with cilia. Molecular analysis based on sequences of 18S rRNA and ITS genes confirmed the ciliate species as Balantidium coli. ...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Two Imported Cases of Babesiosis with Complication or Co-Infection with Lyme Disease in Republic of Korea.
We report 2 cases of severe babesiosis initially mistaken as malaria. The first patient was complicated by shock and splenic infarction, the other co-infected with Lyme disease. As the population traveling abroad increases every year, physicians should be aware of babesiosis which mimics malaria, co-infection with other diseases, and its complications. PMID: 30630283 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China.
In this study, we isolated an Acanthamoeba CJW/W1 strain from tap water in Wuxi, Jiangsu Province, China. Its 18S rDNA was sequenced and a phylogenetic tree was constructed. The isolated cysts belonged to morphologic group II. Comparison of 18S rDNA sequences of CJW/W1 strain and other isolates showed high similarity (99.7%) to a clinical isolate Asp, KA/E28. A phylogeny analysis confirmed this isolate belonged to the pathogenic genotype T4, the most common strain associated with Acanthamoeba-related diseases. This is the first report of an Acanthamoeba strain isolated from tap water in Wuxi, China. Acanthamoeba could be a...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Epidemiological Survey on Eimeria spp. Associated with Diarrhea in Pre-weaned Native Korean Calves.
Authors: Kim HC, Choe C, Kim S, Chae JS, Yu DH, Park J, Park BK, Choi KS Abstract Bovine coccidiosis is one of the most important parasitic diseases affecting calf productivity. Here, we investi- gated the prevalence of Eimeria spp. in pre-weaned native Korean calves and determined the correlation between diar- rhea and Eimeria spp. Fecal samples were collected from individual calves (288 normal and 191 diarrheic) in 6 different farms. Of the 479 samples, Eimeria oocysts were detected in 124 calves (25.9%). Five Eimeria spp. were identified; E. zuernii (18.8%) was the most prevalent, followed by E. auburnensis (12....
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

An Evaluation of Active Case Detection in Malaria Control Program in Kiyuni Parish of Kyankwanzi District, Uganda.
This study assessed the perceptions and practices on malaria control in Kiyuni Parish of Kyankwanzi district, Uganda. Our study presents that the incidence of malaria is sustained high despite efforts to scale-up and improve the use of LLINs and access to ACDs, based on the average incidence confirmed by RDTs. PMID: 30630286 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Infection Status of Isthmiophora hortensis Metacercariae in Dark Sleepers, Odontobutis Species, from Some Water Systems of the Republic of Korea.
Authors: Sohn WM, Na BK, Cho SH, Ju JW Abstract Present study was performed to survey on infection status of Isthmiophora hortensis (formerly Echinostoma hortense) metacercariae (IhMc) in dark sleepers, Odontobutis spp., from some water systems of the Republic of Korea. A total of 237 Odontobutis spp. was collected in the water systems of 5 rivers, i.e., Mangyeong-gang (gang means river), Geum-gang, Tamjin-gang, Seomjin-gang, and Nakdong-gang. They were all examined with artificial digestion method for 5 years (2013-2017). A total of 137 (57.8%) Odontobutis spp. were infected with 14.8 IhMc in average. The prevalen...
Source: Korean Journal of Parasitology - January 12, 2019 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Prevention and Control Strategies for Parasitic Infections in the Korea Centers for Disease Control and Prevention.
Authors: Bahk YY, Shin EH, Cho SH, Ju JW, Chai JY, Kim TS Abstract Korea is successfully controlled intestinal parasitic infections owing to economic development and high health consciousness. The Division of Vectors and Parasitic Diseases (formerly the Division of Malaria and Parasitology) is in the Center for Laboratory Control of Infectious Diseases of the Korea Centers for Disease Control and Prevention. It has been the governmental agency responsible for controlling and leading scientific research on parasitic diseases. The Division of Vectors and Parasitic Diseases has conducted and funded basic research and ...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii.
In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples.
This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes of traveler's diarrhea. Three target genes were specifically and simultaneously detected by the TaqMan probe method for multiple parasitic infection cases, including Cryptosporidium oocyst wall protein for C. parvum, glutamate dehydrogenase for G. lamblia, and internal transcribed spacer 1 for C. cayetanensis. Gene product 21 for bacteriophage T4 was used as an internal control DNA target for monitoring hu...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Virus-Like Particles Expressing Toxoplasma gondii Rhoptry Protein 18 Induces Better Protection Than Rhoptry Protein 4 against T. gondii Infection.
In this study, we generated 2 virus-like particles (VLPs) vaccines expressing T. gondii rhoptry protein 4 (ROP4) or rhoptry protein 18 (ROP18) using influenza matrix protein (M1) as a core protein. Mice were intranasally immunized with VLPs vaccines and after the last immunization, mice were challenged with ME49 cysts. Protective efficacy was assessed and compared by determining serum antibody responses, body weight changes and the reduction of cyst counts in the brain. ROP18 VLPs-immunized mice induced greater levels of IgG and IgA antibody responses than those immunized with ROP4 VLPs. ROP18 VLPs immunization significant...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets.
Authors: Yoo WG, Kim SM, Won EJ, Lee JY, Dai F, Woo HC, Nam HW, Kim TI, Han JH, Kwak D, Cho YS, Kang SW, Kim TS, Zhu XQ, Wang C, Youn H, Hong SJ Abstract To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoi...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Comparative Assessment of Diagnostic Performances of Two Commercial Rapid Diagnostic Test Kits for Detection of Plasmodium spp. in Ugandan Patients with Malaria.
This study was conducted to evaluate the diagnostic performances of the 2 commercially available malaria RDT kits, RapiGEN Malaria Ag Pf/Pv (pLDH/pLDH) and Asan EasyTestTM Malaria Ag Pf/Pv (HRP-2/pLDH) for their abilities to detect Plasmodium species in blood samples collected from Ugandan patients with malaria. To evaluate the diagnostic performances of these 2 RDT kits, 229 blood samples were tested for malaria infection by microscopic examination and a species-specific nested polymerase chain reaction. The detection sensitivities for P. falciparum of Malaria Ag Pf/Pv (pLDH/pLDH) and Asan EasyTestTM Malaria Ag Pf/Pv (HRP...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Distribution Status of Hybrid Types in Large Liver Flukes, Fasciola Species (Digenea: Fasciolidae), from Ruminants and Humans in Vietnam.
Authors: Nguyen TBN, De NV, Nguyen TKL, Quang HH, Doan HTT, Agatsuma T, Le TH Abstract The aim of this study is to delineate 'admixed hybrid' and 'introgressive' Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classifi...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Morphological Studies of Developmental Stages of Oculotrema hippopotami (Monogenea: Polystomatidae) Infecting the Eye of Hippopotamus amphibius (Mammalia: Hippopotamidae) Using SEM and EDXA with Notes on Histopathology.
Authors: Rubtsova NY, Heckmann RA, Smit WJ, Luus-Powell WJ, Halajian A, Roux F Abstract The present study was performed to observe histopathological effects of Oculotrema hippopotami Stunkard, 1924 infection in the eye of Hippopotamus amphibius, as well as to reveal new details of morphology and structural features of this monogenean and its comparison between 2 age stages of the parasite. This was done using both light and scanning electron microscopy, energy dispersive X-ray analysis (EDXA) and histopathology. The presence of a mixture of different generations (adult and sub-adult) in one host individual is commo...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Analysis of Five Arboviruses and Culicoides Distribution on Cattle Farms in Jeollabuk-do, Korea.
Authors: Yang D, Yang MS, Rhim H, Han JI, Oem JK, Kim YH, Lee KK, Lim CW, Kim B Abstract Arthropod-borne viruses (Arboviruses) are transmitted by arthropods such as Culicoides biting midges and cause abortion, stillbirth, and congenital malformation in ruminants, apparently leading to economic losses to farmers. To monitor the distribution of Culicoides and to determine their relationship with different environmental conditions (temperature, humidity, wind speed, and altitude of the farms) on 5 cattle farms, Culicoides were collected during summer season (May-September) in 2016 and 2017, and analyzed for identifica...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Phthirus pubis Infestation of the Scalp: A Case Report and Review of The Literature.
We describe a case of P. pubis infestation located exclusively on the scalp in an adult woman. Neither lice/nits nor skin lesions were observed elsewhere, including eyebrows, eyelashes, axillae, pubis, buttocks and perianal region (the patient was hairless in the axillae and pubis). A review of the literature is enclosed. PMID: 30419734 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions.
In this study, we investigated the possibility of DCB as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba cyst. Eight commercial MPDS (from a to h) were assessed, all of which displayed insufficient amoebicidal activity against the mature cysts. Solution e, f, and h showed strong amoebicidal effect on the immature cysts. Amoebicidal efficacy against mature cysts remained inadequate even when the 8 MPDS were combined with 100 μM DCB. However, 4 kinds of MPDS (solution d, e, f, and h) including 100 μM DCB demonstrated strong amoebicidal activity against the immature cysts. The amoeb...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Prevalence and Phylogenetic Analyses of Trichuris suis in Pigs in Hunan Province, Subtropical China.
The objective of this research was to investigate the prevalence of T. suis in pigs in Hunan province. Total 2,267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions (Hunan province) were evaluated for the existence of T. suis eggs using saturated NaCl floating method. The average infection rate of T. suis in pigs was 8.91% in Hunan province. To determine genetic variation of the gained T. suis isolates in the present study, the internal transcribed spacer (ITS) regions from nuclear ribosomal DNA (rDNA) of 7 T. suis isolates were cloned and analyzed. Nucleotide diversities were 1.0-3.5%...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Morphological Characteristics and Fish Hosts of Isoparorchis sp. (Digenea: Isoparorchiidae) in Korea.
We described here the morphological characteristics for the species identification and fish hosts of Isoparorchis sp. (Digenea: Isoparorchiidae) in the Republic of Korea (Korea). Total 1,371 freshwater fishes collected in Yangcheon (Stream) in Sancheong-gun, Gyeongsangnam-do were examined by the artificial digestion methods to survey the infection status of digenetic trematode metacercariae for 4 years (2013-2016). Adult and larval worms of Isoparorchis sp. were detected in 38 (8.4%) out of 451 fish in 4 species, i.e., Pungtungia herzi, Acheilognathus koreensis, Squalidus japonicus coreanus and Odontobutis platycephala, ex...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Infections with Digenean Trematode Metacercariae in Two Invasive Alien Fish, Micropterus salmoides and Lepomis macrochirus, in Two Rivers in Chungcheongbuk-do, Republic of Korea.
We report here that the 2 alien fish species were less infected with the metacercariae than the native ones. Surveys on the metacercariae in the alien fish species in geographically various rivers should be undertaken for better understanding on the role of alien fish species in the trematode infections in Republic of Korea. PMID: 30419738 [PubMed - in process] (Source: Korean Journal of Parasitology)
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Complete Mitochondrial Genome of the Chagas Disease Vector, Triatoma rubrofasciata.
In this study, we completed the mitochondrial genome sequencing of T. rubrofasciata. The total length of T. rubrofasciata mitochondrial genome was 17,150 bp with the base composition of 40.4% A, 11.6% G, 29.4% T and 18.6% C. It included 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one control region. We constructed a phylogenetic tree on the 13 protein-coding genes of T. rubrofasciata and other 13 closely related species to show their phylogenic relationship. The determination of T. rubrofasciata mitogenome would play an important role in understanding the genetic diversity and evolution of triatomine bugs. ...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Survey and Phylogenetic Analysis of Rodents and Important Rodent-Borne Zoonotic Pathogens in Gedu, Bhutan.
In this study, a cross-sectional investigation of zoonotic disease pathogens in rodents was performed in Chukha district, Bhutan, where a high incidence of scrub typhus and cases of acute undifferentiated febrile illness had been reported in people during the preceding 4-6 months. Twelve rodents were trapped alive using wire-mesh traps. Following euthanasia, liver and kidney tissues were removed and tested using PCR for Orientia tsutsugamushi and other bacterial and rickettsial pathogens causing bartonellosis, borreliosis, human monocytic ehrlichiosis, human granulocytic anaplasmosis, leptospirosis, and rickettsiosis. A ph...
Source: Korean Journal of Parasitology - November 15, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Research Progress on Leptotrombidium deliense.
This article reviews Leptotrombidium deliense, including its discovery and nomenclature, morphological features and identification, life cycle, ecology, relationship with diseases, chromosomes and artificial cultivation. The first record of L. deliense was early in 1922 by Walch. Under the genus Leptotrombidium, there are many sibling species similar to L. deliense, which makes it difficult to differentiate L. deliense from another sibling chigger mites, for example, L. rubellum. The life cycle of the mite (L. deliense) includes 7 stages: egg, deutovum (or prelarva), larva, nymphochrysalis, nymph, imagochrysalis and adult....
Source: Korean Journal of Parasitology - September 12, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Evaluation of Protective Immune Response Induced by a DNA Vaccine Encoding GRA8 against Acute Toxoplasmosis in a Murine Model.
Authors: Chu JQ, Huang S, Ye W, Fan XY, Huang R, Ye SC, Yu CY, Wu WY, Zhou Y, Zhou W, Lee YH, Quan JH Abstract Toxoplasma gondii is an apicomplexan zoonotic protozoan parasite that infects most species of warm-blooded animals, including humans. The heavy incidence and severe or lethal damage caused by T. gondii infection clearly indicate a need for the development of an effective vaccine. T. gondii GRA8 is a member of the dense granules protein family and is used as a marker of acute infection. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic pl...
Source: Korean Journal of Parasitology - September 12, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea.
This study was conducted to investigate the prevalence of intestinal parasites among students from Goseonggun, Gangwon-do and Hadong-gun, Gyeongsangnam-do as typical low and high endemic counties. From May to July 2017, a total of 2,033 fecal samples were collected at 27 elementary, 10 junior high, and 8 high schools from 2 counties and examined by the Kato-Katz technique for egg-positive surveys (Collection rate: 37.02% [2,033/5,492]). Of the participants examined, 13 (0.64%) were found to harbor eggs of 3 parasitic species, Trichuris trichiura, Clonorchis sinensis and Metagonimus yokogawai. Based on the regional distribu...
Source: Korean Journal of Parasitology - September 12, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Infections with Centrocestus armatus Metacercariae in Fishes from Water Systems of Major Rivers in Republic of Korea.
Authors: Sohn WM, Na BK, Cho SH, Ju JW, Kim CH, Yoon KB, Kim JD, Son DC, Lee SW Abstract The infection status of Centrocestus armatus metacercariae (CaMc) was broadly surveyed in freshwater fishes from major river systems in the Republic of Korea (Korea) during 2008-2017. A total of 14,977 fishes was caught and examined by the artificial digestion method. CaMc were detected in 3,818 (97.1%) (2,114 Z. platypus: 96.1% and 1,704 Z. temminckii: 98.4%) out of 3,932 Zacco spp. examined and their density was 1,867 (2,109 in Z. platypus and 1,567 in Z. temminckii) per fish infected. The prevalences with CaMc were high, 93....
Source: Korean Journal of Parasitology - September 12, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research

Infection Status with Metagonimus spp. Metacercariae in Fishes from Seomjin-gang and Tamjin-gang in Republic of Korea.
Authors: Sohn WM, Na BK, Cho SH, Ju JW, Kim CH, Yoon KB Abstract To grasp the infection status of Metagonimus spp. metacercariae (MsMc), the freshwater fishes were surveyed from Seomjin-gang (river) and Tamjin-gang in the Republic of Korea. Total 1,604 fishes from 7 local sites of Seomjin-gang and 1,649 fishes from 2 sites of Tamjin-gang were examined for 6 years (2012-2017) by the artificial digestion method. MsMc were detected in fishes from 7 sites, i.e., Osucheon in Imsil-gun (36.3% fish in 6 spp.), Seomjin-gang in Sunchanggun (49.8% in 18 spp.), Songdaecheon in Namwon-si (64.5% in 8 spp.), Seomjin-gang in Goks...
Source: Korean Journal of Parasitology - September 12, 2018 Category: Parasitology Tags: Korean J Parasitol Source Type: research