Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona.

Effector gene expression underlying neuron subtype-specific traits in the Motor Ganglion of Ciona. Dev Biol. 2019 Oct 19;: Authors: Gibboney S, Orvis J, Kim K, Johnson CJ, Martínez-Feduchi P, Lowe EK, Sharma S, Stolfi A Abstract The central nervous system of the Ciona larva contains only 177 neurons. The precise regulation of neuron subtype-specific morphogenesis and differentiation observed during the formation of this minimal connectome offers a unique opportunity to dissect gene regulatory networks underlying chordate neurodevelopment. Here we compare the transcriptomes of two very distinct neuron types in the hindbrain/spinal cord homolog of Ciona, the Motor Ganglion (MG): the Descending decussating neuron (ddN, proposed homolog of Mauthner Cells in vertebrates) and the MG Interneuron 2 (MGIN2). Both types are invariantly represented by a single bilaterally symmetric left/right pair of cells in every larva. Supernumerary ddNs and MGIN2s were generated in synchronized embryos and isolated by fluorescence-activated cell sorting for transcriptome profiling. Differential gene expression analysis revealed ddN- and MGIN2-specific enrichment of a wide range of genes, including many encoding potential "effectors" of subtype-specific morphological and functional traits. More specifically, we identified the upregulation of centrosome-associated, microtubule-stabilizing/bundling proteins and extracellular guidance cues part of a single int...
Source: Developmental Biology - Category: Biology Authors: Tags: Dev Biol Source Type: research