Green chromatographic methods for simultaneous determination of quetiapine and the co-administrated paroxetine in rat plasma with application to pharmacokinetic study

Publication date: Available online 14 October 2019Source: Microchemical JournalAuthor(s): Nada S. Abdelwahab, Amal B. Ahmed, Mahmoud A. Omar, Sayed M. Derayea, Maha M. AbdelrahmanABSTRACTNewly established green TLC-densitometric and RP-HPLC methods were developed and validated for simultaneous determination of quetiapine (QUT) (an antipsychotic drug) and the co-administrated drug, paroxetine (PAR) (an antidepressant drug). Both drugs are widely prescribed for treatment of bipolar depression. The proposed TLC-densitometric method was based on separation of the studied components and the internal standard (vincamine (VINC)) using a developed system of ethyl acetate: ethanol: ammonia solution 33% (8: 2:0.05, by volume) on TLC silica gel 60 F254 plates and scanning at 240 nm. Linear relationships were obtained over a concentration range of 150-3000 ng/band for both using 1000 ng/mL of vincamine (VINC) as an internal standard. On the other hand, the proposed RP-HPLC method depended on separation of the studied components and the internal standard (vincamine) using a mixture of methanol: 0.05 M KH2PO4 (pH 4.3) (70:30, v/v) on XTerra® HPLC RP C18 column (4.6 mm × 250 mm, 5µm) at a flow rate of 1 mL/min, and UV detection at 240 nm. Linearity was achieved on the concentration range of 150-5000 ng/mL for both using 1000 ng/mL of VINC as an internal standard. The greenness profile of the developed methods was assessed and compared with reported HPLC method using the analytical Ec...
Source: Microchemical Journal - Category: Chemistry Source Type: research