Caffeic acid alkyl amide derivatives ameliorate oxidative stress and modulate ERK1/2 and Akt signaling pathways in a rat model of diabetic retinopathy.

Caffeic acid alkyl amide derivatives ameliorate oxidative stress and modulate ERK1/2 and Akt signaling pathways in a rat model of diabetic retinopathy. Chem Biodivers. 2019 Sep 30;: Authors: Fathalipour M, Eghtedari M, Borges F, Silva T, Moosavi F, Firuzi O, Mirkhani H Abstract The purpose of this study was to examine the neuroprotective effects of caffeic acid hexyl (CAF6) and dodecyl (CAF12) amide derivatives on the early stage of retinopathy in streptozotocin-induced diabetic rats. Animals were divided in 5 groups (n = 8/group); One group consisted of control non-diabetic rats, while the other 4 were diabetic animals either non-treated or treated with CAF6, CAF12 or resveratrol intravitreally for 4 weeks. Retinal superoxide dismutase (SOD) activity and 8- iso prostaglandin F 2α (iPF 2α ) levels were evaluated by an ELISA assay. Phosphorylation of ERK1/2 and AKT were determined by immunoblotting in retinal homogenates. Retinal morphology was also examined using light microscopy. Treatment with CAF6 and CAF12 increased retinal SOD activity, while it decreased iPF 2α levels in diabetic rats. Phosphorylation of ERK1/2 was increased, while AKT phosphorylation was decreased in diabetic rats compared to normal control and these alterations were significantly reversed in diabetic rats treated with CAF6 and CAF12. Furthermore, thickness of the whole retinal layer, outer nuclear layer, and ganglion cell count were decreased in diabetic r...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research