Diagnostic and prognostic values of C ‑X‑C motif chemokine ligand 3 in patients with colon cancer.

Diagnostic and prognostic values of C‑X‑C motif chemokine ligand 3 in patients with colon cancer. Oncol Rep. 2019 Sep 19;: Authors: Ruan GT, Gong YZ, Liao XW, Wang S, Huang W, Wang XK, Zhu GZ, Liao C, Gao F Abstract The diagnostic and prognostic mechanisms of C‑X‑C motif chemokine ligand 3 (CXCL3) in colon cancer (CC) have not yet been reported. Therefore, the objective of the present study was to use cohorts of patients from Guangxi Medical University and the Gene Expression Omnibus (GEO) database to investigate and validate CXCL3 for the diagnosis and prognosis of CC, and to explore its prospective molecular mechanism. Reverse transcription‑quantitative PCR (RT‑qPCR) analysis of 38 paired tumor and non‑tumor tissues, and immunohistochemistry (IHC) of 212 tumor and 46 non‑tumor tissues was conducted to explore the expression of CXCL3 and its diagnostic and prognostic significance in the Guangxi Medical University CC cohort. A GEO dataset, GSE40967, was used to validate the prognostic significance of CXCL3. Gene set enrichment analysis (GSEA) was also conducted to explore the potential molecular mechanisms underlying the effects of CXCL3 in CC. The RT‑qPCR results indicated that CXCL3 expression was significantly higher in cancer tissues compared with adjacent normal tissues, suggesting that it may have high diagnostic value for CC. Multivariate Cox analysis based on the IHC results suggested that there was no appre...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research