Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine.

Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine. Am J Physiol Cell Physiol. 2019 Sep 18;: Authors: Chao T, Gomez BI, Heard TC, Smith BW, Dubick MA, Burmeister DM Abstract Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte mitochondria arecurrently unknown. The purpose of this study is to determine cardiac mitochondrial function in a swine burn model with subsequent resuscitation using either crystalloids or colloids. Anesthetized Yorkshire swine (n=23) sustained 40% total body surface area (TBSA) burns and received IV crystalloids (n=11) or colloids (n=12) after recovery from anesthesia. Non-burned swine served as control (n=9). After euthanasia at 48h, heart tissues were harvested, permeabilized, and analyzed by high-resolution respirometry. Citrate synthase (CS) activity was measured, and Western blots were performed to quantify proteins associated with mitochondrial fusion (OPA1), fission (FIS1), and mitophagy (PINK1). There were no differences in State-2 respiration or maximal oxidative phosphorylation. Coupled Complex-1 respiration decreased, while uncoupled State-4O, and complex-II increased significantly due to bur...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research