Quality assurance for genotyping and resistance testing of Clostridium (Clostridioides) difficile isolates - experiences from the first inter-laboratory ring trial in four German speaking countries.

Quality assurance for genotyping and resistance testing of Clostridium (Clostridioides) difficile isolates - experiences from the first inter-laboratory ring trial in four German speaking countries. Anaerobe. 2019 Sep 05;:102093 Authors: Berger FK, Mellmann A, von Müller L, Bischoff M, Gärtner BC, German speaking C. difficile laboratory study group Abstract Clostridium (Clostridioides) difficile is a major cause of nosocomial diarrhoea. A first inter-laboratory ring trial was performed in four European countries to evaluate the genotyping and antibiotic susceptibility testing (AST) accuracy. Six C. difficile isolates representing the epidemiologic important ribotypes (RT), RT001, RT002, RT010, RT014, RT027, and RT078 were blinded and send to 21 participating laboratories. Participants tested the samples with their genotyping and AST methods in use for concordance with reference. A total of 21 genotyping- and 14 antimicrobial susceptibility data sets were obtained. Ribotyping (11 participants) correctly identified most RTs (median 91% concordance rate) except for RT002, which was misidentified in 4/11 reports. However, this isolate was correctly asserted to RT002 after an update of a publicly available ribotyping database. Multilocus sequence typing, surface layer sequence typing, DNA microarray based genotyping, and whole genome sequencing, which were used by 1-3 participants, identified all six isolates correctly. AST was done by ...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research