Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue.

Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue. Methods Mol Biol. 2020;2058:155-177 Authors: Das K, Urbiola C, Spiesschaert B, Mueller P, Wollmann G Abstract In addition of being directly tumoricidal, oncolytic viruses have emerged as potent partners for established and investigational immunotherapies due to their immune-stimulatory effects. The shifting focus on virus-mediated immune modulation calls for a comprehensive analysis of the tumor microenvironment (TME) and the factors orchestrating the antiviral and antitumor immune response. The oncolytic VSV-GP studied in our lab is a safe and potent antitumor agent with a fast replication cycle and killing of a broad range of different cancer types. It induces a robust local inflammatory conversion of the TME and drives a strong adaptive immune response toward the tumor. Here we present our multidisciplinary approach to study VSV-GP treatment effects in tumors by assessing both immune cells (tumor-infiltrating lymphocytes and tumor-associated macrophages) and immune-regulatory factors (cytokines) as well as characterizing immune signatures using an immune-targeted NanoString gene expression system. PMID: 31486037 [PubMed - in process]
Source: Molecular Medicine - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research