Development of a conserved chimeric vaccine based on helper T-cell and CTL epitopes for induction of strong immune response against Schistosoma mansoni using immunoinformatics approaches.

This study was conducted to construct a multi-epitope based vaccine against Schistosoma mansoni via utilizing Sm14, Sm21.7, Sm23, Sm29, Smp80, Sm-CB and SM-TSP-2 antigens. Helper T lymphocyte (HTL), cytotoxic T lymphocyte (CTL) and IFN-γ epitopes were predicted. Furthermore, Pan HLA DR-binding epitope was added to the vaccine. Moreover, 50S ribosomal protein L7/L12 of Mycobacterium tuberculosis as a novel TLR4 agonist was applied. The TAT peptide was added to the vaccine to augment intracellular delivery. The selected epitopes were linked together through appropriate linkers and chimeric vaccine was constructed with 617 amino acids with molecular weight of 65.43 kDa. Physico-chemical properties revealed a soluble protein with antigenic and non-allergic properties. Further analyses validated the stability of the construct that was able to interact with TLR4. Immunoinformatics analysis demonstrated the strong potential of constructed vaccine to stimulate T and B-cell mediated immune responses. In summary, obtained data indicated that the proposed vaccine can properly induce both T and B cells immune responses and could possibly be utilized for prophylactic or therapeutic aims in response to infection caused by S. mansoni. PMID: 31479669 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

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Conditions:   Mycobacterium Tuberculosis, Protection Against;   Tuberculosis Interventions:   Biological: ChadOx1 85A - aerosol;   Biological: ChadOx1 85A - IM Sponsors:   François Spertini;   University of Oxford Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
CONCLUSION: Although the concept of mandatory vaccination seems to be favoured by many health professionals in Europe, it remains a controversial subject both among HCWs' profession categories and also among different countries. Interventions to increase vaccination among HCWs would benefit by tailoring their approach according to disease and target group. PMID: 31580555 [PubMed - in process]
Source: Central European Journal of Public Health - Category: International Medicine & Public Health Tags: Cent Eur J Public Health Source Type: research
A tuberculosis vaccine commonly used in other parts of the world might reduce a person's risk of developing lung cancer if given early in childhood, a six-decade-long study reports.
Source: WebMD Health - Category: Consumer Health News Source Type: news
TUESDAY, Oct. 1, 2019 -- A tuberculosis vaccine commonly used in other parts of the world might reduce a person's risk of developing lung cancer if given early in childhood, a six-decade-long study reports. The Bacille Calmette-Guerin (BCG) vaccine...
Source: Drugs.com - Daily MedNews - Category: General Medicine Source Type: news
Diagnostic accuracy of interferon (IFN)-γ inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis. Mol Biol Rep. 2019 Sep 28;: Authors: Mamishi S, Mahmoudi S, Banar M, Hosseinpour Sadeghi R, Marjani M, Pourakbari B Abstract To assess the potency of Interferon (IFN)-γ inducible protein 10 (IP-10) stimulated by recombinant PE35 and PPE68 as a biomarker in differentiating between active and latent tuberculosis. Patients with active pulmonary TB (PTB) (n = 30), latent TB infection (LTBI) (n = 29), and BCG-vaccinated healt...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
Research will help advance TB vaccine development.
Source: National Institutes of Health (NIH) News Releases - Category: American Health Source Type: news
In conclusion, he pointed to the “profound” challenges we face, saying: “I am confident that if we continue and strengthen international co-operation and seize the opportunities already available, while creating even more opportunities, we can overcome them – together”.   ‘A political choice’ Universal health coverage means all people regardless of their ability to pay, having access to the health care they need, when and where they need it, without facing financial hardship. Congratulating world leaders, Tedros Adhanom Ghebreyesus, Director-General at the World Health Or...
Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Development & Aid Global Headlines Health Human Rights Source Type: news
Conclusion: Several health maintenance aspects are inadequately addressed by GIs and PCPs, in part because of conflicting perceptions of responsibility. Clear guidelines and better GI/PCP communication are required to ensure effective health maintenance for patients with IBD. PMID: 31528131 [PubMed]
Source: Ochsner Journal - Category: General Medicine Tags: Ochsner J Source Type: research
In this study, we evaluated global M. tuberculosis-induced gene expression in airway immune cells obtained by bronchoalveolar lavage (BAL) of individuals with latent TB infection (LTBI) and M. tuberculosis-naive controls. In prior studies, we demonstrated that BAL cells from LTBI individuals display substantial enrichment for M. tuberculosis-responsive CD4+ T cells compared with matched peripheral blood samples. We therefore specifically assessed the impact of the depletion of CD4+ and CD8+ T cells on M. tuberculosis-induced BAL cell gene expression in LTBI. Our studies identified 12 canonical pathways and a 47-gene signat...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Tags: J Immunol Source Type: research
This series of three papers focuses on the management of drug-resistant tuberculosis, challenges in childhood tuberculosis, and the state of vaccine development.
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
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