A Novel Seed-Dressing Formulation Based on an Improved Mutant Strain of Trichoderma virens, and Its Field Evaluation

Using gamma-ray induced mutagenesis, we have developed a mutant (named G2) of Trichoderma virens that produced 2-3 fold excesses of secondary metabolites, including viridin, viridiol, and some yet-to-be identified compounds. Consequently, this mutant had improved antibiosis against the oomycete test pathogen Pythium aphanidermatum. A transcriptome analysis of the mutant vis-à-vis the wild type strain showed upregulation of several secondary-metabolism related genes. In addition, many genes predicted to be involved in mycoparasitism and plant-interactions were also upregulated. We used tamarind seeds as a mass multiplication medium in solid state fermentation, and using talcum powder as a carrier developed a novel seed dressing formulation. A comparative evaluation of the wild type and the mutant in green house under high disease pressure (using the test pathogen Sclerotium rolfsii) revealed superiority of the mutant over wild type in protecting chickpea (Cicer arietinum) seeds and seedlings from infection. We then undertook extensive field evaluation (replicated micro-plot trials, on-farm demonstration trials, and large scale trials in farmers’ fields) of our mutant-based formulation (named as TrichoBARC) for management of collar rot (S. rolfsii) in chickpea and lentil (Lens culinaris) over multiple locations in India. In certain experiments, other available formulations were included for comparison. This formulation consistently, over multiple locations and years, improve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research