Tracking adiponectin biodistribution via fluorescence molecular tomography indicates increased vascular permeability after streptozotocin-induced diabetes.

Tracking adiponectin biodistribution via fluorescence molecular tomography indicates increased vascular permeability after streptozotocin-induced diabetes. Am J Physiol Endocrinol Metab. 2019 Jul 16;: Authors: Yoon N, Dadson K, Dang T, Chu T, Noskovicova N, Hinz B, Raignault A, Thorin E, Kim S, Jeon JS, Jonkman J, McKee T, Grant J, Peterson JD, Kelly SP, Sweeney G Abstract Adiponectin, highly abundant in plasma, plays an important role in mediating important beneficial effects in diabetes, inflammation and cardiovascular disease. To act on target tissues, adiponectin must move from circulation to the interstitial space, suggesting that vascular permeability plays an important role in regulating adiponectin action. To test this hypothesis, fluorescently-labelled adiponectin was used to monitor its biodistribution in mice with streptozotocin-induced diabetes (STZD). Adiponectin was indeed found to have increased sequestration in the highly fenestrated liver and other tissues within 90 min in STZD mice. In addition, increased myocardial adiponectin was detected and confirmed using Computed Tomography (CT) co-registration. A higher adiponectin content in the STZD heart tissues was further confirmed by ex vivo fluorescence molecular tomography (FMT) imaging, immunohistochemistry and western blot analysis. In vitro mechanistic studies using an endothelial monolayer on inserts and 3D microvascular networks on microfluidic chips further conf...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research