Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells.
Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells. Methods Mol Biol. 2019;1879:385-392 Authors: Hoffman RM Abstract Human hair follicle-associated pluripotent (hHAP) stem cells were isolated from the upper parts of human hair follicles. hHAP stem cells were suspended in DMEM containing 10% fetal bovine serum (FBS) where they differentiated to cardiac muscle cells as well as neurons, glial cells, keratinocytes, and smooth muscle cells. The methods of this chapter are appropriate for use of human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve, and spinal cord regeneration in the clinic. PMID: 29992514 [PubMed - indexed for MEDLINE]
The objective of this clinical trial was to assess the efficacy of allogeneic mesenchymal stem cells (MSCs) in inducing Tr1 cells and suppressing AAA inflammation.
The objective of this study was to assess efficacy and safety of a prepared autologous stem cell product delivered as a split dose by intra-arterial infusion and intramuscular injection in patients with critical limb ischemia.
In this study, we differentiated hiPSC-NSPCs into neurons and evaluated the susceptibility of hiPSC-neurons and parental hiPSC-NSPCs to anticancer drugs in vitro by ATP assay and immunocytostaining. The hiPSC-neurons were more resistant to anticancer drugs than the parental hiPSC-NSPCs. In the toxicity tests, high-dose cisplatin reduced the levels of ELAVL3/4, a neuronal marker, in the hiPSC-neurons. These results suggest that our methodology is potentially applicable for efficient determination of the toxicity of any drug to hiPSC-neurons.
We present a case of a 55-year-old female diagnostic radiologist who had previously received an HSCT for Philadelphia chromosome-positive acute lymphoblastic lymphoma.
This study represents that the exosomes derived from ASCs (ASC-exo) play an important role in human dermal fibroblasts by stimulating its cell cycle.
Background: Exosome, a relatively recently founded 30- to 150-nm-sized extracellular vesicle, is secreted from most cells and acts as a communicator between them. We would like to explore whether exosomes from mesenchymal stem cells (MSCs) could be used as a novel therapeutic option for hair loss.
Introduction: Options for therapy of severe burns are limited and generally include local wound care, debridement, grafting, and long-term care. Mesenchymal stem cells (MSCs) have been used in applications directed at the repair and regeneration of damaged tissue. There is little known regarding the clinical application of allogenic MSCs in the treatment of second degree burn injury. This pilot study establishes the safety of MSC therapy for burn wounds in two dose escalations, in addition to providing both observational and objective data that this treatment protocol improves the healing of these injuries.
We report multiple allogeneic HSCT recipients with lichenoid cGVHD with extensive photodamage and photocarcinogenesis to the skin, with histopathologic findings demonstrating predominant features of lichenoid cGVHD.
Thermal injury leads to loss of the regenerative capacity of the skin and scarring becomes a significant risk, due to the inability to emulate normal skin architecture and adnexal structures. Mesenchymal stem cells (MSC) have been used in a variety of clinical applications to repair and regenerate damaged tissue. MSC ’s can stimulate cellular processes such as fibroblast proliferation, migration, and endothelial angiogenesis, as well as modulate the host’s immune response to favor more optimal wound healing.
A 48-year-old woman with a history of AIDS and stage IV Hodgkin lymphoma status post chemotherapy and stem cell transplant presented with 8 months of painful perineal erosions and exophytic plaques. Her medical history was significant for acyclovir-resistant herpes simplex virus (HSV) and multiple opportunistic infections. Examination revealed diffuse discontinuous eroded plaques in the perineum, and scattered skin-colored and hyperpigmented exophytic plaques with raised borders and central craters overlying the sacrum, gluteal cleft, and perineum.