Control of bacterial and fungal biofilms by natural products of Ziziphus joazeiro Mart. (Rhamnaceae).

Control of bacterial and fungal biofilms by natural products of Ziziphus joazeiro Mart. (Rhamnaceae). Comp Immunol Microbiol Infect Dis. 2019 Aug;65:226-233 Authors: Cosmo Andrade J, da Silva ARP, Audilene Freitas M, de Azevedo Ramos B, Sampaio Freitas T, de Assis G Dos Santos F, Leite-Andrade MC, Nunes M, Relison Tintino S, da Silva MV, Dos Santos Correia MT, de Lima-Neto RG, Neves RP, Melo Coutinho HD Abstract The aim of this study is to verify the action of the aqueous leaf extract Ziziphus joazeiro in the eradication of bacterial and fungal biofilms, and to compare these effects with the stem bark extracts, as well as with conventional standard drugs. The presence of secondary metabolites was observed through phytochemical prospection assays. The effect of the aqueous extract on microbial biofilm formation was observed by OD600 nm absorbance and the crystal violet assay. For bacterial and fungal biofilms, chlorhexidine gluconate and fluconazole, respectively, were used as positive controls. Phytochemical characterization showed the presence of secondary metabolite classes common to both extracts such as flavonoids, steroids and saponins. In particular, in the aqueous leaf extract phenols, condensed tannins and alkaloids were observed. Eradication results using the aqueous leaf extract showed an inhibition of the microbial biofilm mass, moreover the biofilms were more sensitive to the bark extract, which presented a greater inhi...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research