Graphene oxide ‐l‐arginine nanogel: A pH‐sensitive fluorouracil nanocarrier

In this study, a pH ‐sensitive graphene oxide‐l‐arginine nanogel was synthesized to carry and release 5‐fluorouracil. Optimized conditions using statistical analysis, based on the maximum relative viscosity of nanogel, were evaluated: 5.489 for the concentration ofl‐arginine and 2.404 for pH. The prepared nanogels were characterized using scanning electron microscope and transmission electron microscope images and Fourier‐transform infrared spectroscopic analysis. Cytotoxicity was assessed using the sulforhodamine B (SRB) assay on MCF‐7 breast cancer cel ls. The fluorouracil release was measured by the dialysis bag method, UV spectrophotometry, and fluorouracil calibration diagram. Results proved the successful controlled release of fluorouracil at pH 5.4 and the beneficial role of graphene‐oxide‐l‐arginine‐ fluorouracil nanogel in eliminating cancer cells.

Source: Biotechnology and Applied Biochemistry - June 16, 2019 Category: Biochemistry Authors: Hanieh Malekimusavi, AmirHossein Ghaemi, Ghasem Masoudi, Faraz Chogan, Hamid Rashedi, Fatemeh Yazdian, Meisam Omidi, Shohreh Javadi, Bibi Fatemeh Haghiralsadat, Masoumeh Teimouri, Naghmeh Faal Hamedani Tags: Original Article Source Type: research