Multifunctional gelatin–tricalcium phosphate porous nanocomposite scaffolds for tissue engineering and local drug delivery: In vitro and in vivo studies

Publication date: Available online 27 May 2019Source: Journal of the Taiwan Institute of Chemical EngineersAuthor(s): Mehdi Rahmanian, Amir seyfoori, Mohammad Mehdi Dehghan, Leila Eini, Seyed Morteza Naghib, Hossein Gholami, Saeed Farzad, Kaveh Rahimi Mamaghani, Keivan Majidzadeh-AAbstractBone is known as the most prevalent, metastatic site of breast cancer; however, it's mechanism details are unknown, so developing a suitable scaffold for bone defect regeneration and sustain drug release after tumor resection can considerably inhibit the tumor recurrence after a while. Here, synthesized gelatin/beta-tricalcium phosphate (β-TCP) nanocomposite scaffold loaded with zoledronic acid (ZA) drug with different β-TCP concentrations, crosslinker and varying drug doses were investigated to highlight their effect on biological properties of the scaffold contained bone cells. The scanning electron microscopy (SEM) illustrated porous structure (50–200 µm) of gelatin reinforced with β-TCP spherical nanoparticles (around 90 nm diameter) that could be a proper matrix for bone cells proliferation. In vitro biological analysis by cytotoxicity assay (MTT test) as well as osteoblast cell line (G292) attachment test showed that the scaffolds were biocompatible and non-toxic. Also, higher β-TCP nanoparticle concentration enhanced the rate of cell proliferation on the scaffold. Further investigations indicated that the type of cell seeding into porous scaffolds should be considered as a si...
Source: Journal of the Taiwan Institute of Chemical Engineers - Category: Chemistry Source Type: research