Establishment of efficient differentiation conditions from tonsil-derived mesenchymal stem cells to motor neuron cells

ConclusionAfter the MN differentiating in our own methods, we confirmed that the expression of MN-related markers, including ISL1, HB9, ChAT in T-MSC-MNCs has increased compared to T-MSCs. Also, to test their functional efficiency, the change of acetylcholine secretion in the culture medium under the differentiation into T-MSC-MNCs was measured. Furthermore, by confirming the acetylcholine receptor clusters when co-culturing T-MSC-MNCs and human skeletal muscle cells, which was demonstrated the T-MSC-MNCs could form neuromuscular junctions. The functional improvements afforded by these T-MSC-MNCs are potentially useful in the treatment of human MNDs caused by genetic, viral, and environmental problems.
Source: Cytotherapy - Category: Cytology Source Type: research