Effect of interferon-γ-primed mesenchymal stem cells on the growth of acute lymphoblastic leukemia cells in an in vivo model

ConclusionWe established four distinct stable cell lines expressing firefly luciferase (fLuci) gene by infection of lentivirus in Jurkat, CCRF-CEM, Sup-T1 and CCRF-HSB2 ALL cells. 105 ALL/fLuci cells together with 106 MSCs were inoculated into NOD/SCID mice by intraperitoneal (IP) injection. And we measured luciferase activity using an IVIS optical imaging technique to determine the effects of MSCs on leukemic mass formation. Much higher luciferase activity was observed when ALL/fLuci cells were co-injected with MSCs as compared to that when ALL/fLuci cells were inoculated alone. In addition, we co-inoculated 105 ALL/fLuci cells with different numbers of MSCs (1:1, 1:5 or 1:10 ratio) into mice. Although the bioluminescent intensity gradually increased in all groups, mice that were co-injected with 106 MSCs showed a higher bioluminescent intensity than those co-injected with lower number of MSCs, indicating the possibility that MSCs play an important role in stimulating a leukemic proliferation. To determine the effects of IFN-γ- priming on the growth of leukemic cells by MSCs, ALL/fLuci cells together with IFN-γ-primed MSCs were inoculated into NOD/SCID mice via intraperitoneal injection. Lower luciferase activity was observed when ALL/fLuci cells were co-injected with IFN-γ-primed MSCs as compared to that when ALL/fLuci cells were co-inoculated with naive MSCs. These results suggest that IFN-γ-priming may activate MSCs’ potential anti-cancer effects. Further studies ar...
Source: Cytotherapy - Category: Cytology Source Type: research