Effect of interferon-γ-primed mesenchymal stem cells on the growth of acute lymphoblastic leukemia cells in an in vivo model

ConclusionWe established four distinct stable cell lines expressing firefly luciferase (fLuci) gene by infection of lentivirus in Jurkat, CCRF-CEM, Sup-T1 and CCRF-HSB2 ALL cells. 105 ALL/fLuci cells together with 106 MSCs were inoculated into NOD/SCID mice by intraperitoneal (IP) injection. And we measured luciferase activity using an IVIS optical imaging technique to determine the effects of MSCs on leukemic mass formation. Much higher luciferase activity was observed when ALL/fLuci cells were co-injected with MSCs as compared to that when ALL/fLuci cells were inoculated alone. In addition, we co-inoculated 105 ALL/fLuci cells with different numbers of MSCs (1:1, 1:5 or 1:10 ratio) into mice. Although the bioluminescent intensity gradually increased in all groups, mice that were co-injected with 106 MSCs showed a higher bioluminescent intensity than those co-injected with lower number of MSCs, indicating the possibility that MSCs play an important role in stimulating a leukemic proliferation. To determine the effects of IFN-γ- priming on the growth of leukemic cells by MSCs, ALL/fLuci cells together with IFN-γ-primed MSCs were inoculated into NOD/SCID mice via intraperitoneal injection. Lower luciferase activity was observed when ALL/fLuci cells were co-injected with IFN-γ-primed MSCs as compared to that when ALL/fLuci cells were co-inoculated with naive MSCs. These results suggest that IFN-γ-priming may activate MSCs’ potential anti-cancer eff...
Source: Cytotherapy - Category: Cytology Source Type: research

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Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Mathew L. Cooper, John F. DiPersioAbstractAt present, the only curative therapy for patients with T-cell malignancies is allogeneic stem cell transplant, which has associated risks and toxicities. Novel agents have been tried in relapsed T-cell acute lymphoblastic leukemia (T-ALL), but only one, with 20%-30% complete remission rates, has been approved by the US Food and Drug Administration. T-ALL is a heterogeneous disease, but it has universal overexpression of CD7 as well as several other T-cell markers, s...
Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
Authors: Yu Z, Liu L, Shu Q, Li D, Wang R Abstract Leukemia stem cells (LSCs) are responsible for therapeutic failure and relapse of acute lymphoblastic leukemia. As a result of the interplay between LSCs and bone marrow mesenchymal stem cells (BM-MSCs), cancer cells may escape from chemotherapy and immune surveillance, thereby promoting leukemia progress and relapse. The present study identified that the crosstalk between LSCs and BM-MSCs may contribute to changes of immune phenotypes and expression of hematopoietic factors in BM-MSCs. Furthermore, Illumina Genome Analyzer/Hiseq 2000 identified 7 differentially ex...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Publication date: Available online 15 September 2019Source: Advances in Biological RegulationAuthor(s): Samuel Gusscott, Francesco Tamiro, Vincenzo Giambra, Andrew P. WengAbstractT-cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer, characterized by an uncontrolled expansion and accumulation of T-cell progenitors. During leukemic progression, immature T cells grow abnormally and occupy the bone marrow compartment, thereby interfering with the production of normal blood cells. Pediatric T-ALL is curable with intensive chemotherapy, but there are significant, long-term side effects and ∼20% of patients suf...
Source: Advances in Biological Regulation - Category: Biology Source Type: research
In conclusion, these recent studies strongly support CD123 as an important therapeutic target for the treatment of BPDCN, while a possible in the treatment of AML and other hematological malignancies will have to be evaluated by in the ongoing clinical studies.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
In conclusion, endoglin may assist in the diagnosis and pathogenesis study of various processes associated with mast cells. An endoglin-neutralizing treatment for solid cancers and leukemia could also affect mastocytes and the immunologic system.
Source: Applied Immunohistochemistry and Molecular Morphology - Category: Chemistry Tags: Research Articles Source Type: research
Current therapies for T cell acute lymphoblastic leukaemia (T-ALL) have achieved relatively successful results. However, relapse cases are often fatal. Relapse is caused by the presence of leukemic stem cells that are resistant to treatment. Using the recently described model of T-ALL pre-leukemic stem cells, the NUP98-HOXD13 (NHD13) mouse, we investigated novel mechanisms of self-renewal in these cells.We found that the thymocytes in these mice overexpressed EphA3, a gene associated with abnormal self-renewal in other cancers.
Source: Experimental Hematology - Category: Hematology Authors: Tags: 3111 Source Type: research
Contributors : Cates Mallaney ; Elizabeth L Ostrander ; Hamza Celik ; Ashley C Kramer ; Andrew Martens ; Alok Kothari ; Won K Koh ; Emily Haussler ; Grant A ChallenSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusRecent studies have indicated that the histone 3 lysine 27 (H3K27me2/3) demethylase KDM6B (JMJD3) is frequently upregulated in a myriad of blood disorders including myelodysplastic syndrome (MDS), T-cell acute lymphoblastic leukemia (T-ALL), and multiple myeloma (MM) suggesting it may have important functions i...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
In conclusion, we demonstrate that QC depletes BCR-ABL protein and suppresses Ph-positive leukemia cells in vitro and in vivo. PMID: 31296070 [PubMed - as supplied by publisher]
Source: Cancer Investigation - Category: Cancer & Oncology Tags: Cancer Invest Source Type: research
The Notch pathway plays a key role in several processes including stem-cell self-renewal, proliferation, and cell differentiation. Several studies identified recurrent mutations in hematological malignancies making Notch one of the most desirable target in leukemia and lymphoma. The Notch signaling mediates resistance to therapy and controls cancer stem cells supporting the development of on-target therapeutic strategies to improve patients ’ outcome. In this brief review, we outline the therapeutic potential of targeting Notch pathway in T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia and mantle cell lymphoma.
Source: Mediterranean Journal of Hematology and Infectious Diseases - Category: Hematology Source Type: research
Neuroblastoma is a common extracranial solid tumor of neural crest (NC) origin that accounts for up to 15% of all pediatric cancer deaths. The disease arises from a transient population of NC cells that undergo an epithelial-mesenchymal transition (EMT) and generate diverse cell-types and tissues. Patients with neuroblastoma are characterized by their extreme heterogeneity ranging from spontaneous regression to malignant progression. More than half of newly diagnosed patients present highly metastatic tumors and are stratified into a high-risk group with dismal outcome. As many as 20% of high-risk patients have residual di...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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