Tissue distribution study of Angelica dahurica cv. Yubaizhi in rat by ultra–performance liquid chromatography with tandem mass spectrometry

Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): You-Bo Zhang, Gai-Gai Deng, Tian-Xia Wang, Lu Liu, Xiu-Wei YangAbstractA sensitive and specific ultra–performance liquid chromatographic–tandem mass (UPLC–MS/MS) spectrometric method was established to investigate tissue distribution of fourteen coumarins of Angelica dahurica cv. Yubaizhi roots (ADYR) in rat tissues, including isoimperatorin (1), imperatorin (2), isooxypeucedanin (3), byakangelicin (4), oxypeucedanin hydrate (5), bergapten (6), 2''R–neobyakangelicol (7), phellopterin (8), xanthotoxin (9), isopimpinellin (10), oxypeucedanin ethanolate (11), isobyakangelicol (12), columbianetin (13), (–)–marmesin (14). Detection was performed on a triple quadrupole mass spectrometer in multiple–reaction–mode (MRM). The method established in this assay was successfully applied to tissue distribution study of the selected 14 coumarins after oral administration of the extract of ADYR in rat tissues, including heart, liver, spleen, lung, kidney, stomach, small intestine, muscle, testis, and brain. Tissue distribution characteristics of the fourteen coumarins were clearly elucidated, and the results of this study indicated that the fourteen coumarins were distributed to rat tissues rapidly and could be detected in all of the selected tissues after oral administration. Concentrations of the coumarins were obviously higher in kidney, liver and stomach tissu...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research