Acute toxicity of triflumizole to freshwater green algae Chlorella vulgaris

Publication date: Available online 15 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jiejun Xi, Jian Shao, Yuan Wang, Xiaoqin Wang, Hui Yang, Xinwei Zhang, Dongmei XiongAbstractTriflumizole is one of imidazole fungicides that works by inhibiting ergosterol biosynthesis, and is widely used for the control of powdery mildew and scabs on various fruits and crops. Triflumizole residue has been frequently detected in soil and aquatic ecosystems. While many studies have focused on its toxic effect on terrestrial and aquatic animals, little attention has been paid to aquatic algae, the primary producers of aquatic environments. Therefore, we evaluated the acute (96 h) toxicity effects of triflumizole on the freshwater algae Chlorella vulgaris, by examining growth, cell morphology, photosynthesis, and oxidative stress. The results showed that the 96 h median inhibition concentration (96 h-EC50) were 0.82 mg/L (95% confidential interval 0.70–0.98 mg/L).The growth of algal cells was conspicuously inhibited by triflumizole exposure, and the cell surfaces appeared to be shrunkThe chlorophyll content (including Chl-a, Chl-b and T-Chl) dramatically decreased at triflumizole concentrations of 0.2 and 1.0 mg/L. In addition, the transcript abundance of photosynthesis-related genes (psaB, psbC and rbcL) showed obvious decreases in above treatments after 96 h of exposure to triflumizole. Moreover, the algal growth inhibition was accompanied by an increase in int...
Source: Pesticide Biochemistry and Physiology - Category: Biochemistry Source Type: research