Ivermectin induces apoptosis of porcine trophectoderm and uterine luminal epithelial cells through loss of mitochondrial membrane potential, mitochondrial calcium ion overload, and reactive oxygen species generation
In this study, we investigated the detrimental effects of ivermectin on porcine trophectoderm (pTr) and uterine luminal epithelial (pLE) cells. Ivermectin not only inhibited the proliferation of both cells via the regulation of cell cycle-associated genes, but also induced apoptosis in pTr and pLE cells. We also verified its effect on mitochondrial dysfunction as shown by loss of mitochondrial membrane potential, mitochondrial Ca2+ overload, and reactive oxygen species (ROS) generation in pTr and pLE cells. As a mechanistic approach, we evaluated ivermectin-mediated cell signaling interactions including PI3K, AKT and MAPK ...
Source: Pesticide Biochemistry and Physiology - June 18, 2019 Category: Biochemistry Source Type: research

Cytochrome P450s Cyp4p1 and Cyp4p2 associated with the DDT tolerance in the Drosophila melanogaster strain 91-R
In this study, we used a comparative approach to demonstrate that transcription of Cyp4p1 and Cyp4p2 were constitutively up-regulated in the Drosophila melanogaster strain 91-R as compared to another DDT susceptible strain Canton-S which does not have a loss of function of these genes. Furthermore, significantly increased expression of Cyp4p1 and Cyp4p2 was induced in 91-R in response to sublethal DDT exposure, however, such induction did not occur in the DDT treated Canton-S. Additionally, fixed nucleotide variation within putative transcription factor binding sites of Cyp4p1 and Cyp4p2 promoters were observed between 91-...
Source: Pesticide Biochemistry and Physiology - June 15, 2019 Category: Biochemistry Source Type: research

Cross-resistance profiles of malaria mosquito P450s associated with pyrethroid resistance against WHO insecticides
Publication date: Available online 13 June 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Cristina Yunta, Kay Hemmings, Bradley Stevenson, Lizette L. Koekemoer, Tonderi Matambo, Pat Pignatelli, Michael Voice, Szilárd Nász, Mark J.I. PaineAbstractExtensive use of pyrethroids for malaria control in Africa has led to widespread pyrethroid resistance in the two major African vectors of malaria An. gambiae and An. funestus. This is often associated with constitutively elevated levels of cytochrome P450s involved with pyrethroid metabolism and detoxification. P450s have the capacity to metabolise diver...
Source: Pesticide Biochemistry and Physiology - June 13, 2019 Category: Biochemistry Source Type: research

Propolis relieves the cardiotoxicity of chlorpyrifos in diabetic rats via alleviations of paraoxonase-1 and xanthine oxidase genes expression
Publication date: Available online 12 June 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Khairy A. Ibrahim, Soad A. Khwanes, Mohamed A. El-Desouky, Heba K.A. ElhakimAbstractPesticides cardiotoxicity in case of diabetic-induced cardiac complications is unidentified. The probable amelioration role of propolis is gauged against the cardiotoxic effects of chlorpyrifos in the diabetic rats through paraoxonase-1 (PON1) and xanthine oxidase (XO) genes dysregulation. Fifty-six male rats were distributed (n = 7) into eight groups. The first one saved as control whereas the 2nd, 3rd, and 4th were kept for propolis ...
Source: Pesticide Biochemistry and Physiology - June 12, 2019 Category: Biochemistry Source Type: research

Latrophilin participates in insecticide susceptibility through positively regulating CSP10 and partially compensated by OBPC01 in Tribolium castaneum
Publication date: Available online 6 June 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Wenfeng Xiong, Shanshan Gao, Yaoyao Lu, Luting Wei, Jinjuan Mao, Jia Xie, Quanquan Cao, Juanjuan Liu, Jingxiu Bi, Xiaowen Song, Bin LiAbstractLatrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their resistance against dichlorvos or carbofuran insecticides. However, the regulatory mech...
Source: Pesticide Biochemistry and Physiology - June 6, 2019 Category: Biochemistry Source Type: research

Copper-induced H2O2 accumulation confers larval tolerance to xanthotoxin by modulating CYP6B50 expression in Spodoptera litura
In this study, we shown that prior exposure to copper (Cu) enhanced larval tolerance to xanthotoxin in Spodoptera litura, a major polyphagous pest of agriculture. P450 activities were induced in larvae exposed to Cu or xanthotoxin, and a midgut specific expressed P450 gene, CYP6B50 was cross-induced after exposure to these two toxic xenobiotics. Knocking down CYP6B50 by RNA interference (RNAi) rendered the larvae more sensitive to xanthotoxin. As defense against oxidative stress following metal exposure has been demonstrated to affect insecticide resistance, the reactive oxygen species (ROS) generation and antioxidant enzy...
Source: Pesticide Biochemistry and Physiology - June 6, 2019 Category: Biochemistry Source Type: research

Transcriptome profiling analysis of the intoxication response in midgut tissue of Agrotis ipsilon larvae to Bacillus thuringiensis Vip3Aa protoxin
Publication date: Available online 4 June 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jinbo Zhang, Haitao Li, Jiali Tan, Panpan Wei, Shuang Yu, Rongmei Liu, Jiguo GaoAbstractVip insecticidal proteins are produced by Bacillus thuringiensis (Bt) during its vegetative growth phase. In the present study, Vip3Aa11 and Vip3Aa39 proteins were investigated. These two proteins present 39 amino acid differential sites and they shared 95.06% amino acid sequence similarity. They are effective against some Lepidoptera insect larvae. In a previous study, using artificial diet bioassays, we estimated the LC50 of Vip3Aa11 ...
Source: Pesticide Biochemistry and Physiology - June 5, 2019 Category: Biochemistry Source Type: research

UDP-glucosyltransferases potentially contribute to imidacloprid resistance in Aphis gossypii glover based on transcriptomic and proteomic analyses
In conclusion, UGTs potentially contributed to imidacloprid resistance in A. gossypii originating from cotton-growing regions of China. These results provide insights into the way we study insecticide resistance in cotton aphids.Graphical abstract (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - June 5, 2019 Category: Biochemistry Source Type: research

RNAi targeting ecdysone receptor blocks the larva to adult development of Tetranychus cinnabarinus
Publication date: Available online 31 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Guang-Mao Shen, Wen Chen, Chuan-Zhen Li, Shi-Yuan Ou, Lin HeAbstractRNA interference (RNAi) is a potentially useful pest control method because of its high specificity. Silencing the expression of important RNAi target genes of pests will block important biological processes and reduce pest damage. Ecdysone is a unique arthropod hormone and the ecdysone receptor (EcR) is a key factor in molting pathway. We investigated the possibility that dsRNA targeting of the EcR of Tetranychus cinnabarinus (TcEcR) could effectively blo...
Source: Pesticide Biochemistry and Physiology - June 2, 2019 Category: Biochemistry Source Type: research

Organophosphorus pesticide triazophos: A new endocrine disruptor chemical of hypothalamus-pituitary-adrenal axis
In this study, adult male Wistar rats were exposed to TAP at the dose of 0.164 and 1.64 mg/kg bodyweight (~1/500th and 1/50th of LD50) for 24 weeks and serum contents of hormones were measured. TAP exposure significantly reduced serum contents of adrenocorticotropic hormone, corticosterone and epinephrine in rats (p 
Source: Pesticide Biochemistry and Physiology - June 2, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: June 2019Source: Pesticide Biochemistry and Physiology, Volume 157Author(s): (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - May 30, 2019 Category: Biochemistry Source Type: research

Altered regulation of porphyrin biosynthesis and protective responses to acifluorfen-induced photodynamic stress in transgenic rice expressing Bradyrhizobium japonicum Fe-chelatase
We examined the molecular regulation of porphyrin biosynthesis and protective responses in transgenic rice (Oryza sativa) expressing Bradyrhizobium japonicum Fe-chelatase (BjFeCh) after treatment with acifluorfen (AF). During the photodynamic stress imposed by AF, transcript levels of BjFeCh in transgenic plants increased greatly; moreover, transcript levels of OsFeCh2 remained almost constant, whereas in wild type (WT) plants they were considerably down-regulated. In the heme branch, transgenic plants exhibited greater levels of OsFC and HO transcripts than WT plants in the untreated stems as well as in the AF-treated lea...
Source: Pesticide Biochemistry and Physiology - May 28, 2019 Category: Biochemistry Source Type: research

Age- and diet-specific effects of chronic exposure to chlorpyrifos on hormones, inflammation and gut microbiota in rats
This study helped clarify the relationship between disrupted endocrine function and gut microbiota dysbiosis induced by food contaminants such as pesticides.Graphical abstract (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - May 28, 2019 Category: Biochemistry Source Type: research

Cross-resistance pattern to ACCase-inhibiting herbicides in a novel Trp1999Leu mutation American sloughgrass (Beckmannia syzigachne) population
Publication date: Available online 28 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Bingqi Liu, Fei Ding, Mingliang Wang, Fei Wang, Xiaoyong Luo, Lingxu LiAbstractThe plastid acetyl coenzyme carboxylase (ACCase) Trp1999Leu mutation was identified in a Beckmannia syzigachne population resistant to fenoxaprop-p-ethyl. The pattern of cross-resistance for the Trp1999Leu mutation is still ambiguous. In this paper, mutant homozygote (1999Leu/Leu, RR) and wild type (1999Trp/Trp, SS) B. syzigachne plants with the same genetic background were purified from the JS-26 population using the dCAPS method. The activity ...
Source: Pesticide Biochemistry and Physiology - May 28, 2019 Category: Biochemistry Source Type: research

Anti-phytopathogenic activity and the possible mechanisms of action of isoquinoline alkaloid sanguinarine
In this study, the antifungal activity of twelve isoquinoline alkaloids, including berberine (1), jatrorrhizine (2), coptisine (3), corydaline (4), tetrahydroberberine (5), chelidonine (6), dihydrosanguinarine (7), chelerythrine (8), sanguinarine (9), palmatine (10), tetrahydropalmatine (11) and columbamine (12) were evaluated against eight plant pathogenic fungi in vitro. All the tested compounds showed varying degrees of inhibition against the eight tested plant fungi. Among them, sanguinarine exhibited high antifungal activity (EC50 ranging from 6.96–59.36 μg/mL). It displayed the best inhibitory activity aga...
Source: Pesticide Biochemistry and Physiology - May 27, 2019 Category: Biochemistry Source Type: research

Antifungal activity of zedoary turmeric oil against Phytophthora capsici through damaging cell membrane
In this study, we found ZTO could inhibit P. capsici growth and development in vitro and in detached cucumber and Nicotiana benthamiana leaves. Besides, ZTO treatment resulted in severe damage to the cell membrane of P. capsici, leading to the leakage of intracellular contents. ZTO also induced a significant increase in relative conductivity, malondialdehyde concentration and glycerol content. Furthermore, we identified 50 volatile organic compounds from ZTO, and uncovered Curcumol, β-elemene, curdione and curcumenol with strong inhibitory activities against mycelial growth of P. capsici. Overall, our results not only...
Source: Pesticide Biochemistry and Physiology - May 25, 2019 Category: Biochemistry Source Type: research

Structure-antifungal relationships and preventive effects of 1-(2,4-dihydroxyphenyl)-2-methylpropan-1-one derivatives as potential inhibitors of class-II fructose-1,6-bisphosphate aldolase
Publication date: Available online 24 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Tuong Thi Mai Luong, Wei-Wei Wang, Fan Zhang, Wen-Jia Dan, Hoang Xuan Nien, An-Ling Zhang, Ding Li, Jin-Ming GaoAbstractEmerging fungal phytodiseases are a food security threat and novel fungicides are in an urgent need. Herein, a series of isobutyrophenone derivatives were designed and synthesized. The derivatives exhibited excellent fungicidal activities against seven fungi. The structure-activity relationship (SAR) study indicated that the introduction of a bromo group at the position 3 or 5 of the phenyl ring, as well ...
Source: Pesticide Biochemistry and Physiology - May 25, 2019 Category: Biochemistry Source Type: research

Potential of fungal metabolites as a biocontrol agent against cotton aphid, Aphis gossypii Glover and the possible mechanisms of action
Publication date: Available online 22 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Azza A. Elbanhawy, Elsherbiny A. Elsherbiny, Ahmed E. Abd El-Mageed, Gamal M. Abdel-FattahAbstractThe present study investigated the insecticidal activity of the different organic extracts from the entomopathogenic fungi, Cladosporium cladosporioides, Metarhizium anisopliae, Purpureocillium lilacinum, and Trichoderma longibrachiatum towards cotton aphid, Aphis gossypii. The methanol extracts from the mycelia and spores of C. cladosporioides and P. lilacinum exhibited the highest insecticidal activity against A. gossypii co...
Source: Pesticide Biochemistry and Physiology - May 24, 2019 Category: Biochemistry Source Type: research

In silico chemical library screening and experimental validation of novel compounds with potential varroacide activities
In this study, we have been looking for a new original varroacide treatment inhibiting selectively Varroa destructor AChE (vdAChE) with respect to Apis mellifera AChE (amAChE). To do this an original drug design methodology was used applying virtual screening of the CERMN chemolibrary, starting from a vdAChE homology sequence model. By combining the in silico screening with in vitro experiments, two promising compounds were found. In vitro tests of AChE inhibition for both species have confirmed good selectivity toward the mite vdAChE. Moreover, an in vivo protocol was performed and highlighted a varroacide activity withou...
Source: Pesticide Biochemistry and Physiology - May 24, 2019 Category: Biochemistry Source Type: research

Differential metabolism of imidacloprid and dinotefuran by Bemisia tabaci CYP6CM1 variants
Publication date: Available online 20 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Akira Hamada, Gregory D. Wahl, Alexandre Nesterov, Toshifumi Nakao, Miyuki Kawashima, Shinichi BanbaAbstractImidacloprid has been used to control one of most serious pests, Bemisia tabaci. However, B. tabaci has developed imidacloprid resistance mainly by over-expressing CYP6CM1. It was reported that imidacloprid-resistant B. tabaci showed no or low level of cross-resistance against dinotefuran. Here, we expressed CYP6CM1 variants using Sf9/baculovirus and/or Drosophila S2 cells and showed that CYP6CM1 variants metabolized...
Source: Pesticide Biochemistry and Physiology - May 21, 2019 Category: Biochemistry Source Type: research

Discovery of N-(4-fluoro-2-(phenylamino)phenyl)-pyrazole-4-carboxamides as potential succinate dehydrogenase inhibitors
Publication date: Available online 21 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Aigui Zhang, Ying Yue, Yihua Yang, Jian Yang, Ke Tao, Hong Jin, Taiping HouAbstractSuccinate dehydrogenase (SDH), an essential component of cellular respiratory chain and tricarboxylic acid (or Krebs) cycle, has been identified as one of the most significant targets for pharmaceutical and agrochemical. Herein, with the aim of discovery of new antifungal lead structures, a class of novel N-(4-fluoro-2-(phenylamino)phenyl)-pyrazole-4-carboxamides were designed, synthesized and evaluated for their biological activities. They ...
Source: Pesticide Biochemistry and Physiology - May 21, 2019 Category: Biochemistry Source Type: research

Resistance evolution and mechanisms to ALS-inhibiting herbicides in Capsella bursa-pastoris populations from China
This study demonstrated that target site gene mutations were the main reason for Capsella bursa-pastoris resistance to ALS-inhibiting herbicides. Although target-site mutation is the reason for resistance to ALS-inhibiting herbicides in Capsella bursa-pastoris, the resistance patterns and mutations identified have changed over time.Graphical abstract (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - May 18, 2019 Category: Biochemistry Source Type: research

Predicting herbicide movement across semi-permeable membranes using three phase partitioning
Publication date: Available online 17 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Hudson K. Takano, Eric L. Patterson, Scott J. Nissen, Franck E. Dayan, Todd A. GainesAbstractHerbicide efficacy depends on herbicides crossing cell and organelle membranes. We evaluated an artificial membrane system to understand how herbicides cross biological membranes. This understanding aids in predicting herbicide behavior in planta and, consequently, efficacy, mode of action, and whether active transporter-based herbicide resistance mechanisms may be possible. Five herbicides with different log Kow and pKa values wer...
Source: Pesticide Biochemistry and Physiology - May 18, 2019 Category: Biochemistry Source Type: research

Acute toxicity of triflumizole to freshwater green algae Chlorella vulgaris
Publication date: Available online 15 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jiejun Xi, Jian Shao, Yuan Wang, Xiaoqin Wang, Hui Yang, Xinwei Zhang, Dongmei XiongAbstractTriflumizole is one of imidazole fungicides that works by inhibiting ergosterol biosynthesis, and is widely used for the control of powdery mildew and scabs on various fruits and crops. Triflumizole residue has been frequently detected in soil and aquatic ecosystems. While many studies have focused on its toxic effect on terrestrial and aquatic animals, little attention has been paid to aquatic algae, the primary producers of aquati...
Source: Pesticide Biochemistry and Physiology - May 16, 2019 Category: Biochemistry Source Type: research

Potential of hydrocarbon and oxygenated monoterpenes against Culex pipiens larvae: Toxicity, biochemical, pharmacophore modeling and molecular docking studies
Publication date: Available online 16 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Nehad E.M. Taktak, Mohamed E.I. BadawyAbstractCulex pipiens is a main vector for Bancroftian filariasis, Rift Valley Fever and diseases caused by other viruses, leaving several peoples with disabilities. In recent years, plant derived compounds have received much attention as potential alternatives to synthetic chemicals due to their low toxicity to mammals and environmental persistence. Twenty-one monoterpenes from different chemical groups (hydrocarbons and oxygenated products) were evaluated against Culex pipiens larvae...
Source: Pesticide Biochemistry and Physiology - May 16, 2019 Category: Biochemistry Source Type: research

Antimicrobial and antioxidant activities of hydrocarbon and oxygenated monoterpenes against some foodborne pathogens through in vitro and in silico studies
Publication date: Available online 16 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Mohamed E.I. Badawy, Gehan I.Kh. Marei, Entsar I. Rabea, Nehad E.M. TaktakAbstractThe present work describes the antimicrobial action of 25 monoterpenes (six hydrocarbons, five ketones, two aldehydes, six alcohols and six acetate analogues) against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus and antifungal activity against Aspergillus flavus. The antibacterial activity was evaluated by broth microdilution technique as a minimum inhibitory concentration (MIC) and the antifungal activity was perfor...
Source: Pesticide Biochemistry and Physiology - May 16, 2019 Category: Biochemistry Source Type: research

Bio-efficacy of chitinolytic Bacillus thuringiensis isolates native to northwestern Indian Himalayas and their synergistic toxicity with selected insecticides
Publication date: Available online 14 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): A.R.N.S. Subbanna, C. Chandrashekara, J. Stanley, K.K. Mishra, P.K. Mishra, A. PattanayakAbstractPesticidal properties of Bacillus thuringiensis and its associated toxic proteins is an ever-growing science with potential implications in biological pest management. In the present study 80 Bacillus thuringiensis isolates native to Uttarakhand Himalayas were evaluated for chitinolytic activity and potent ones (11 isolates) were further subjected to multiphasic characterization for their antifungal, insecticidal and synergisti...
Source: Pesticide Biochemistry and Physiology - May 16, 2019 Category: Biochemistry Source Type: research

Molecular basis of resistance to ACCase-inhibiting herbicide cyhalofop-butyl in Chinese sprangletop (Leptochloa chinensis (L.) Nees) from China
Publication date: Available online 11 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Wei Deng, Jingxuan Cai, Jingyun Zhang, Yueyang Chen, Yongrui Chen, Yingjie Di, Shuzhong YuanAbstractChinese sprangletop (Leptochloa chinensis (L.) Nees) is one of the most troublesome grass weeds in rice in China. Seven suspected cyhalofop-butyl-resistant L. chinensis populations were collected from different rice fields with a history of cyhalofop-butyl use. The level of resistance and resistance mechanisms in seven populations were studied. Dose-response tests indicated that five populations (JS3, JS4, JS6, JS7 and JS8) ...
Source: Pesticide Biochemistry and Physiology - May 12, 2019 Category: Biochemistry Source Type: research

Use of Ganoderma Lucidum polysaccharide to control cotton fusarium wilt, and the mechanism involved
In this study, we found that GLP spray and irrigation root treatments can promote growth in cotton. After soaking in GLP, theseedling height and cotton fusarium wilt resistance both increased to some extent, effects that were dose dependent. After treatment of cotton with GLP, the activities of peroxidase (POD), superoxide dismutase (SOD) and polyphenol oxidase (PPO) in leaves increased significantly, whereas the content of malondialdehyde (MDA) decreased. In addition, QRT-PCR results showed significantly increased relative expression of genes related to the jasmonic acid pathway in cotton. Therefore, we speculate that GLP...
Source: Pesticide Biochemistry and Physiology - May 12, 2019 Category: Biochemistry Source Type: research

Synthesis and bioactivity of indoleacetic acid-carbendazim and its effects on Cylindrocladium parasiticum
Publication date: Available online 11 May 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jingmei Yang, Tao Ye, Guanghua Liu, Xiaoting Xu, Yixiong Zheng, Wenkai WangAbstractIndoleacetic acid (IAA)-carbendazim was synthesized to assess whether this conjugate could retain the fungicidal activity of carbendazim and gain root-inducing properties upon the addition of an indoleacetic acid group. An indoor virulence test demonstrated that the conjugate retained the fungicidal activity of carbendazim towards Cylindrocladium parasiticum. The conjugate was detected in roots after soaking Ricinus communis L. leaves into a...
Source: Pesticide Biochemistry and Physiology - May 12, 2019 Category: Biochemistry Source Type: research

Modelling of the methyl halide biodegradation in bacteria and its effect on environmental systems
In this study, we have investigated the in-silico pathways of methyl halide and its toxic effect on living systems like pest, humans and environment. Till date, limited studies provide the understanding of degradation of methyl halide and its effect on the environment. This leads to availability of scanty information for overall bio-magnifications of methyl halides at molecular and cellular level. The model developed in the present study explains how a volatile toxic compound not only affects living systems on earth but also on environmental layers. Hub nodes were also evaluated by investigating the developed model topolog...
Source: Pesticide Biochemistry and Physiology - May 7, 2019 Category: Biochemistry Source Type: research

Transcriptome profiling to identify cytochrome P450 genes involved in penoxsulam resistance in Echinochloa glabrescens
In this study, we found that the GR50 of the resistant population (SHQP-R) decreased substantially from 25.6 to 5.0 and 6.2 g a.i. ha−1 after treatment with the P450 inhibitors piperonyl butoxide (PBO) and malathion, respectively. However, P450 inhibitors almost had no effects on the susceptibility of the sensitive population (JYJD-S) to penoxsulam. To investigate the mechanisms of metabolic resistance, transcriptome sequencing analysis was performed to find candidate genes that may confer resistance to penoxsulam in E. glabrescens. A total of 233 P450 differentially expressed genes (DEGs) were identified by transc...
Source: Pesticide Biochemistry and Physiology - May 4, 2019 Category: Biochemistry Source Type: research

A bioactivity and biochemical analysis of iminoctadine tris (albesilate) as a fungicide against Corynespora cassiicola
In this study, baseline sensitivity data for mycelial growth showed that the frequency distribution curve of iminoctadine tris (albesilate) EC50 values is unimodal. The EC50 values ranged from 0.1151 to 1.2101 μg/mL, with a mean of 0.5775 ± 0.2677 μg/mL. Iminoctadine tris (albesilate) affected the morphological development of C. cassiicola, causing increased branching of the mycelium. The significant increase in membrane permeability and malondialdehyde content after iminoctadine tris (albesilate) treatment indicated that this fungicide caused severe damage to the membrane structure. Furthermore, 0.4...
Source: Pesticide Biochemistry and Physiology - May 2, 2019 Category: Biochemistry Source Type: research

New approaches to old problems: Removal of phospholipase A2 results in highly active microsomal membranes from the honey bee, Apis mellifera
Publication date: Available online 30 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Marion Zaworra, Ralf NauenAbstractOver the last 50 years numerous studies were published by insect toxicologists using native microsomal membrane preparations in order to investigate in vitro cytochrome P450-(P450) mediated oxidative metabolism of xenobiotics, including insecticides. Whereas the preparation of active microsomal membranes from many pest insect species is straightforward, their isolation from honey bees, Apis mellifera (Hymenoptera: Apidae) remained difficult, if not impossible, due to the presence of a ...
Source: Pesticide Biochemistry and Physiology - May 1, 2019 Category: Biochemistry Source Type: research

The unusual action of essential oil component, menthol, in potentiating the effect of the carbamate insecticide, bendiocarb
Publication date: Available online 30 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Milena Jankowska, Bruno Lapied, Waldemar Jankowski, Maria StankiewiczAbstractStandard chemical insecticides present mainly neurotoxic effects and are becoming less and less effective due to insects developing resistance to them. One of the innovative strategies to control insects pests is to find a way to increase the sensitivity of the target sites in the insect nervous system to the applied insecticides. In the presented research, we proposed menthol, a component of essential oils, as a factor increasing the effectiven...
Source: Pesticide Biochemistry and Physiology - May 1, 2019 Category: Biochemistry Source Type: research

T929I and K1774N mutation pair and M918L single mutation identified in the voltage-gated sodium channel gene of pyrethroid-resistant Thrips tabaci (Thysanoptera: Thripidae) in Japan
Publication date: Available online 25 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Akiya Jouraku, Seigo Kuwazaki, Hiroyuki Iida, Izumi Ohta, Hisao Kusano, Motonori Takagi, Tomoya Yokoyama, Naoya Kubota, Manabu Shibao, Kanako Shirotsuka, Akeo Iwasaki, Yuji Takezawa, Mitsuyoshi TakedaAbstractPyrethroid-resistance in onion thrips, Thrips tabaci, has been reported in many countries including Japan. Identifying factors of the resistance is important to correctly monitoring the resistance in field populations. To identify pyrethroid-resistance related genes in T. tabaci in Japan, we performed RNA-Seq analysi...
Source: Pesticide Biochemistry and Physiology - April 26, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: May 2019Source: Pesticide Biochemistry and Physiology, Volume 156Author(s): (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - April 25, 2019 Category: Biochemistry Source Type: research

Functional characterization of pheromone receptors in the moth Athetis dissimilis (Lepidoptera: Noctuidae)
Publication date: Available online 22 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Xiao-Long Liu, Si-Jie Sun, Sajjad Ali Khuhro, Mohammed Esmail Abdalla Elzaki, Qi Yan, Shuang-Lin DongAbstractSex pheromones are crucial for communication between females and males in moths, and pheromone receptors (PRs) play a key role in peripheral coding of sex pheromones. During the last decade, many PR candidates have been identified based on transcriptome sequencing and bioinformatic analysis, but their detailed functions remain mostly unknown. Here, focusing on four PR candidates of Athetis dissimilis (AdisOR1, Adi...
Source: Pesticide Biochemistry and Physiology - April 23, 2019 Category: Biochemistry Source Type: research

Cross-resistance and biochemical characterization of buprofezin resistance in the white-backed planthopper, Sogatella furcifera (Horvath)
Publication date: Available online 20 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Ehsan Ali, Kaikai Mao, Xun Liao, Ruoheng Jin, Jianhong LiAbstractBuprofezin is a chitin synthesis inhibitor that is very effective against Homopteran pests, such as the white-backed planthopper (WBPH), S. furcifera (Horvath). In the present study, resistance selection, cross-resistance and mechanisms of buprofezin resistance were investigated in this planthopper species. However, the mechanism associated with resistance to growth regulator insecticides (IGRs) remains largely unknown. A resistant strain (Bup-R) with a res...
Source: Pesticide Biochemistry and Physiology - April 21, 2019 Category: Biochemistry Source Type: research

Insecticide resistance monitoring of house fly populations from the United States
Publication date: Available online 20 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jamie C. Freeman, Douglas H. Ross, Jeffrey G. ScottAbstractInsecticide resistance in house fly populations is a major problem faced by livestock producers worldwide. A survey of insecticide resistance levels and pyrethroid resistance allele frequencies in the United States was conducted in 2008–09, but little is known about how resistance levels have changed over the last 10 years. In addition, new target-site pyrethroid resistance alleles that confer high levels of resistance have been recently identified in the...
Source: Pesticide Biochemistry and Physiology - April 21, 2019 Category: Biochemistry Source Type: research

Pyrethroid resistance in the pest mite, Halotydeus destructor: Dominance patterns and a new method for resistance screening
Publication date: Available online 19 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Xuan Cheng, Paul A. Umina, Siu Fai Lee, Ary A. HoffmannAbstractAn L1024F substitution in the para gene, which encodes a subunit of the voltage-gated sodium channel, has been implicated in pyrethroid resistance in a mite pest, Halotydeus destructor, which attacks rape and other grain crops. A high-resolution melt (HRM) genotyping assay was developed for testing the relative pyrethroid susceptibility of different para genotypes and for high-throughput field screening of resistant alleles. The L1024F mutation was found to b...
Source: Pesticide Biochemistry and Physiology - April 20, 2019 Category: Biochemistry Source Type: research

A long non-coding RNA regulates cadherin transcription and susceptibility to Bt toxin Cry1Ac in pink bollworm, Pectinophora gossypiella
Publication date: Available online 19 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Shengyun Li, Fiaz Hussain, Gopalan C. Unnithan, Shuanglin Dong, Zain UlAbdin, Shaohua Gu, Lolita G. Mathew, Jeffrey A. Fabrick, Xinzhi Ni, Yves Carrière, Bruce E. Tabashnik, Xianchun LiAbstractExtensive planting of transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) has spurred increasingly rapid evolution of resistance in pests. In the pink bollworm, Pectinophora gossypiella, a devastating global pest, resistance to Bt toxin Cry1Ac produced by transgenic cotton is linke...
Source: Pesticide Biochemistry and Physiology - April 20, 2019 Category: Biochemistry Source Type: research

Fitness costs of sulfoxaflor resistance in the cotton aphid, Aphis gossypii Glover
Publication date: Available online 19 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Kangsheng Ma, Qiuling Tang, Jin Xia, Nannan Lv, Xiwu GaoAbstractAphis gossypii Glover is an economically important pest of numerous crops throughout the world. Some field populations of A.gossypii in China have developed moderate level of resistance to sulfoxaflor, a newly released sulfoximine insecticide for management of sap-feeding pests. To evaluate the effect of sulfoxaflor resistance on the fitness cost of A. gossypii, the life history traits of sulfoxaflor-resistant strain (SulR) and an isogenic susceptible strain...
Source: Pesticide Biochemistry and Physiology - April 20, 2019 Category: Biochemistry Source Type: research

Enhancement of glyphosate efficacy on Amaranthus palmeri by exogenous quinate application
Publication date: Available online 13 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Ainhoa Zulet-González, Manuel Fernández-Escalada, Ana Zabalza, Mercedes RoyuelaGraphical abstract (Source: Pesticide Biochemistry and Physiology)
Source: Pesticide Biochemistry and Physiology - April 14, 2019 Category: Biochemistry Source Type: research

RDL A301S alone does not confer high levels of resistance to cyclodiene organochlorine or phenyl pyrazole insecticides in Plutella xylostella
Publication date: Available online 13 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): M. Guest, J.A. Goodchild, J.A. Bristow, A.J. FlemmingAbstractMutations in the GABA-gated chloride channel are associated with resistance to cyclodiene organochlorine and phenyl pyrazole insecticides. The best characterised of these is A301S, which was initially identified in a Dieldrin resistant strain of Drosophila melanogaster. The orthologous mutation has been found in a variety of different crop pests including the diamond back moth Plutella xylostella. However, the contribution of this mutation to resistance in this...
Source: Pesticide Biochemistry and Physiology - April 14, 2019 Category: Biochemistry Source Type: research

Sodium pheophorbide a has photoactivated fungicidal activity against Pestalotiopsis neglecta
Publication date: Available online 10 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Yang Jing, Lin Lian-Nan, Zhang Xiao-Bo, Wu Yue, Bi Bing, Zhang Guo-Cai, Zou Chuan-ShanAbstractSodium pheophorbide a (SPA) is a natural photosensitizer. To explore its antifungal activity and mechanism, we studied its inhibitory effects on spore germination and mycelial growth of Pestalotiopsis neglecta. We used sorbitol, 2-thiobarbituric acid (TBA) and electron microscopy to determine its effects on cell wall integrity, cell membrane lipid peroxidation and mycelial morphology. Finally, the effects of SPA on enzyme activi...
Source: Pesticide Biochemistry and Physiology - April 11, 2019 Category: Biochemistry Source Type: research

Diversity in species composition and fungicide resistance profiles in Colletotrichum isolates from apples
Publication date: Available online 6 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): A. Chechi, J. Stahlecker, M.E. Dowling, G. SchnabelAbstractOutbreaks of bitter rot were observed in three commercial apple orchards in Illinois despite best management efforts during the 2018 production season. Three isolates from symptomatic fruit from these orchards and two isolates from an orchard in South Carolina were identified to the species level using morphological tools and calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and beta-tubulin gene sequences. The isolates from Illinois were identified as Colleto...
Source: Pesticide Biochemistry and Physiology - April 7, 2019 Category: Biochemistry Source Type: research

Molecular cloning, characterization, and expression profiling analysis of Cry toxin receptor genes from sugarcane shoot borer Chilo infuscatellus (Snellen)
Publication date: Available online 3 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Jin-da Wang, Jia-song Zhang, Yan-Fang Guo, Li-fei Chen, Fa-Lv Wang, Mei-ting Huang, San-ji Gao, Ran WangAbstractThe sugarcane shoot borer Chilo infuscatellus (Snellen) is known for causing severe damage to sugarcane yield in China. Methods have been developed to control this pest, including Cry toxin pesticide and transgenic Bt plants. In order to investigate the molecular mechanism of the Cry toxin binding process and provide a basis for understanding the insect's resistance mechanism, we used a high throughput sequencin...
Source: Pesticide Biochemistry and Physiology - April 5, 2019 Category: Biochemistry Source Type: research

Cytochrome P450 monooxygenases-mediated sex-differential spinosad resistance in house flies Musca domestica (Diptera: Muscidae)
In this study, the potential role of cytochrome P450 monooxygenases involved in the sex-differential spinosad resistance in house flies was investigated, using a susceptible strain (SSS) and a spinosad resistant near-isogenic line (N-SRS). Combination of the synergism of spinosad by PBO and increased cytochrome P450 monooxygenase activity in the N-SRS strain implied that cytochrome P450 monooxygenases contributed to spinosad resistance in house flies. Transcriptional levels of eight P450 genes related to insecticide resistance in two genders of the SSS and N-SRS strain were separately evaluated by quantitative real-time PC...
Source: Pesticide Biochemistry and Physiology - April 3, 2019 Category: Biochemistry Source Type: research

Functional characterization of CYP6A51, a cytochrome P450 associated with pyrethroid resistance in the Mediterranean fruit fly Ceratitis capitata
Publication date: Available online 1 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Dimitra Tsakireli, Maria Riga, Stella Kounadi, Vassilis Douris, John VontasAbstractOverexpression of the cytochrome P450 monooxygenase CYP6A51 has been previously associated with pyrethroid resistance in the Mediterranean fruit fly (medfly) Ceratitis capitata, an important pest species worldwide; however, this association has not been functionally validated. We expressed CYP6A51 gene in Escherichia coli and produced a functional enzyme with preference for the chemiluminescent substrate Luciferin-ME EGE. In vitro metabolis...
Source: Pesticide Biochemistry and Physiology - April 2, 2019 Category: Biochemistry Source Type: research