Kinase inhibitors Tyrphostin 9 and rottlerin block early steps of rabies virus cycle.

Kinase inhibitors Tyrphostin 9 and rottlerin block early steps of rabies virus cycle. Antiviral Res. 2019 May 06;: Authors: Lama Z, Gaudin Y, Blondel D, Lagaudriere-Gesbert C Abstract Rabies virus (RABV) is a neurotropic virus that causes fatal encephalitis in humans and animals and still kills up to 59,000 people worldwide every year. To date, only preventive or post-exposure vaccination protects against the disease but therapeutics are missing. After screening a library of 80 kinases inhibitors, we identified two compounds as potent inhibitors of RABV infection: Tyrphostin 9 and Rottlerin. Mechanism of action studies show that both inhibitors interfere with an early step of viral cycle and can prevent viral replication. In presence of Tyrphostin 9, the viral entry through endocytosis is disturbed leading to improper delivery of viral particles in cytoplasm, whereas Rottlerin is inhibiting the transcription, most likely by decreasing intracellular ATP concentrations, and therefore the replication of the viral genome. PMID: 31071352 [PubMed - as supplied by publisher]
Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research

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This article provides information on these and other travel vaccinations against hepatitis A, typhoid fever, rabies, Japanese encephalitis and cholera.Yellow fever endemic areas are located in Africa and in South America; there is no yellow fever in Asia. The meningococcal vaccine (A, C, W, Y) is required for pilgrims to Saudi Arabia. Additionally, it is recommended for travellers visiting the African "meningitis belt" during the dry season. A polio booster is required for countries with endemic wild-type polio virus (WPV) or circulating vaccine derived poliovirus (cVDPV).Hepatitis A is a common vaccine-prev...
Source: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz - Category: International Medicine & Public Health Authors: Tags: Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz Source Type: research
Publication date: Available online 7 May 2019Source: Antiviral ResearchAuthor(s): Zoé Lama, Yves Gaudin, Danielle Blondel, Cécile Lagaudriere-GesbertAbstractRabies virus (RABV) is a neurotropic virus that causes fatal encephalitis in humans and animals and still kills up to 59,000 people worldwide every year. To date, only preventive or post-exposure vaccination protects against the disease but therapeutics are missing. After screening a library of 80 kinases inhibitors, we identified two compounds as potent inhibitors of RABV infection: Tyrphostin 9 and Rottlerin. Mechanism of action studies show that both i...
Source: Antiviral Therapy - Category: Virology Source Type: research
In this study, we standardized a real-time quantitative RT-PCR duplex assay to be used during intermediate stages of the vaccine production. This assay was done for the quantification of vaccine strain rabies virus, targeting rabies nucleoprotein, and β-actin mRNA of BHK-21 cells as an internal endogenous control. The results showed specific amplification, with the analytical sensitivity ranged from 101 to 106 TCID50/mL with high repeatability rate for the quantification of rabies virus in inactivated vaccine samples. Global organizations are engaged to develop new approaches to determine viable residual virus, and th...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
shley Banyard Lyssaviruses constitute a diverse range of viruses with the ability to cause fatal encephalitis known as rabies. Existing human rabies vaccines and post exposure prophylaxes (PEP) are based on inactivated preparations of, and neutralising antibody preparations directed against, classical rabies viruses, respectively. Whilst these prophylaxes are highly efficient at neutralising and preventing a productive infection with rabies virus, their ability to neutralise other lyssaviruses is thought to be limited. The remaining 15 virus species within the lyssavirus genus have been divided into at least three phyl...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Across China, the virus that could spark the next pandemic is already circulating. It’s a bird flu called H7N9, and true to its name, it mostly infects poultry. Lately, however, it’s started jumping from chickens to humans more readily–bad news, because the virus is a killer. During a recent spike, 88% of people infected got pneumonia, three-quarters ended up in intensive care with severe respiratory problems, and 41% died. What H7N9 can’t do–yet–is spread easily from person to person, but experts know that could change. The longer the virus spends in humans, the better the chance that i...
Source: TIME.com: Top Science and Health Stories - Category: Consumer Health News Authors: Tags: Uncategorized CDC Disease ebola Gates Foundation MERS outbreak pandemic Zika Source Type: news
CONCLUSION: Pre-exposure rabies prophylaxis is safe and immunogenic and should be considered: (i) where access to postexposure prophylaxis is limited or delayed; (ii) where the risk of exposure is high and may go unrecognized; and (iii) where controlling rabies in the animal reservoir is difficult. Pre-exposure prophylaxis should not distract from canine vaccination efforts, provision of postexposure prophylaxis or education to increase rabies awareness in local communities. PMID: 28250534 [PubMed - in process]
Source: Bulletin of the World Health Organization - Category: Global & Universal Authors: Tags: Bull World Health Organ Source Type: research
INTRODUCTION: Rabies is acute encephalitis caused by rabies virus and is transmissible between humans and warm blooded animals. The virus is belong to the family of Rabdoviridae and, of Lissavirus genus. AIM: To find out the success of rabies surve...
Source: SafetyLit - Category: Global & Universal Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
Abstract: Rabies is an acute infection of the nervous system in human and animals caused by rabies virus. Transmission usually occurs via a bite of an infected animal; transmission via aerosols and by tissue or organ transplantation are rare. Worldwide, endemic dog rabies is responsible for the vast majority of human cases, particularly in Asia and Africa. There are about 60,000 fatal human cases annually, and almost half are in children. Dog rabies can become well controlled by mass vaccination of dogs, which has proved to be very successful in Latin America.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Type: Invited Presentation Source Type: research
by Brecht Devleesschauwer, Arjun Aryal, Barun Kumar Sharma, Anita Ale, Anne Declercq, Stephanie Depraz, Tara Nath Gaire, Gyanendra Gongal, Surendra Karki, Basu Dev Pandey, Sher Bahadur Pun, Luc Duchateau, Pierre Dorny, Niko Speybroeck Background Rabies is a vaccine-preventable viral zoonosis belonging to the group of neglected tropical diseases. Exposure to a rabid animal may result in a fatal acute encephalitis if effective post-exposure prophylaxis is not provided. Rabies occurs worldwide, but its burden is disproportionately high in developing countries, including Nepal. We aimed to summarize current knowledge on the ep...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
ConclusionGiven the insufficient time for completion and relatively low vaccination rates, greater awareness of earlier pre‐travel consultations, at least 4–6 weeks before travel, and accurate risk assessment for travelers are important. Effective counseling about vector avoidance, rabies, and animal bite prevention and management remains critical.
Source: Journal of Travel Medicine - Category: Infectious Diseases Authors: Tags: ORIGINAL ARTICLE Source Type: research
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