Enzyme-conjugated gold nanoparticles for combined enzyme and photothermal therapy of colon cancer cells

Publication date: Available online 12 April 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Mostafa Akbarzadeh Khiavi, Azam Safary, Ayuob Aghanejad, Jaleh Barar, Seyed Hossein Rasta, Asal Golchin, Yadollah Omidi, Mohammad Hossein SomiAbstractThe current investigation introduces an advanced and effective tumor therapeutic RnaGNPs nanobiomedicine composed of gold nanoparticles (GNPs) conjugated with bovine pancreatic ribonuclease (RNase A). The engineered nanobiomedicine was used to overcome the intracellular ribonuclease inhibitors (RIs) as one of the most important obstacles for the clinical applications of RNases in cancer therapy. The RnaGNPs nanobiomedicine was characterized and evaluated for its biological impacts and photothermal effects in the SW-480 colon cancer cells. To determine the efficacy of RnaGNPs combined with the photothermal therapy (PTT), low doses of the near-infrared (NIR) laser irradiation were utilized. The RnaGNPs induced significant cytotoxicity, cell cycle arrest in the sub-G1 phase and a reduction in the G0/G1 and G2/M phases. The RnaGNPs was able to upregulate PTEN, Bax, and caspase 3 and downregulate AKT and Bcl-xL, indicating their key influence in the induction of apoptosis in cancer cells. Mechanistically, the RnaGNPs suppressed the cells' invasion through the downregulation of the extracellular signal-regulated kinases (ERK1/2) pathway. Once combined with PTT, RnaGNPs induced an augmented impact on cancer...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research