Influence of clomiphene citrate on induced ovarian hyperstimulation and subsequent fertility in Damascus goats

Publication date: Available online 5 April 2019Source: Small Ruminant ResearchAuthor(s): S.A. Rateb, I.S. Abd El-Hamid, M.A. Khalifa, N.H. Ibrahim, F. Younis, M. El-RayesAbstractEfficiency of substituting conventional gonadotropin-based superovulation with a clomiphene-based regimen in goats was investigated. Forty pluriparous Damascus does were synchronized for estrus by double doses of PGF2α, 125 µg each, 10 days apart and were randomly divided into 3 groups. The first group served as control (n = 12), whereas superovulation was induced in the second group (n = 14) by 600 mg oral administration of clomiphene. The third group (n = 14) received 600 i.u eCG intramuscularly. Both superovulation treatments were delivered in serial equal doses over 3 consecutive days. Ovarian follicular activity was daily monitored by trans-rectal ultrasonography until the follicle(s) reached the pre-ovulatory phase (Ø ≥ 5 mm), where ovulation was promoted by 20 µg GnRH i.m. Estrus occurrence was observed, and does that exhibited estrus were naturally mated soon after GnRH treatment and 24 h later. The changes in reproductive hormones, blood metabolites and enzymatic activities were also monitored alongside ovarian ultrasonography. Ultrasound examination was conducted 72 h after GnRH treatment to confirm ovulation, as well at day 35 post mating for early pregnancy diagnosis. Reproductive performance indices; i.e. estrus response, ovulation rate, conception rate, kidd...
Source: Small Ruminant Research - Category: Zoology Source Type: research