Differentially Expressed miRNAs Influence Metabolic Processes in Pituitary Oncocytoma.

Differentially Expressed miRNAs Influence Metabolic Processes in Pituitary Oncocytoma. Neurochem Res. 2019 Apr 03;: Authors: Krokker L, Nyírő G, Reiniger L, Darvasi O, Szücs N, Czirják S, Tóth M, Igaz P, Patócs A, Butz H Abstract Spindle cell oncocytomas (SCO) of the pituitary are rare tumors accounting for 0.1-0.4% of all sellar tumors. Due to their rarity, little information is available regarding their pathogenesis. Our aim was to investigate miRNA expression profile of pituitary oncocytomas. Total RNA was extracted from 9 formalin-fixed paraffin embedded pituitary samples (4 primary, 3 recurrent oncocytomas and 2 normal tissues). Next-generation sequencing was performed for miRNA profiling. Transcriptome data of additional 6 samples' were obtained from NBCI GEO database for gene expression reanalysis and tissue-specific target prediction. Bioinformatical analysis, in vitro miRNA mimics transfection, luciferase reporter system and AlamarBlue assay were applied to characterize miRNA's function. 54 differentially expressed miRNAs and 485 genes in pituitary SCO vs. normal tissue and 8 miRNAs in recurrent vs. primary SCO were determined. Global miRNA downregulation and decreased level of DROSHA were detected in SCO samples vs. normal tissue. Transcriptome analysis revealed cell cycle alterations while miRNAs influenced mainly metabolic processes (tricarboxylic acid cycle-TCA, carbohydrate, lipid metabolism). Through miRNA-target...
Source: Neurochemical Research - Category: Neuroscience Authors: Tags: Neurochem Res Source Type: research