Effect of zinc, copper and resveratrol supplementation on the urinary level of 15-hydroxyeicosatetraenoic acid at the early stage of breast cancer in rats.

Effect of zinc, copper and resveratrol supplementation on the urinary level of 15-hydroxyeicosatetraenoic acid at the early stage of breast cancer in rats. J Biol Regul Homeost Agents. 2018 01 18;33(1): Authors: Bobrowska-Korczak B, Skrajnowska D, Seidel K, Bielecki W, Gadzinski B Abstract The aim of the present study was to assess the effect of zinc and copper, separately or in combination with resveratrol, on the concentration of 15-hydroxyeicosatetraenoic acid (15-HETE) in urine of rats with mammary cancer (adenocarcinoma) induced with 7,12- dimethyl-1,2-benz[a]anthracene (+ DMBA). The research focused on the kinetics of alterations in urinary 15-HETE at the early stage of carcinogenesis, as well as on the influence of dietary factors on the process. The content of 15-hydroxyeicosatetraenoic acid in the rats' urine was determined by enzyme-linked immunosorbent assay (ELISA). The 15-HETE level was standardized by conversion to the creatinine level. Regardless of the diet (standard, Zn, Zn+resveratrol, Cu, Cu+resveratrol), DMBA-induced breast carcinogenesis was not inhibited. On the contrary, in the Zn+resveratrol supplemented group, tumorigenesis developed at a considerably faster rate. It was found that the supplementation of the rats' diet with zinc only or zinc plus resveratrol resulted in a reduction of the 15-HETE level in urine of rats with mammry cancer (+ DMBA) as compared with control rats (- DMBA). A statistically lower c...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research